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Q3JE87 (RBL_NITOC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase large chain

Short name=RuBisCO large subunit
EC=4.1.1.39
Gene names
Name:cbbL
Ordered Locus Names:Noc_0333
OrganismNitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) [Complete proteome] [HAMAP]
Taxonomic identifier323261 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity. HAMAP-Rule MF_01338

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP-Rule MF_01338

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP-Rule MF_01338

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01338

Subunit structure

Heterohexadecamer of 8 large chains and 8 small chains By similarity.

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity.

Sequence similarities

Belongs to the RuBisCO large chain family. Type I subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Ribulose bisphosphate carboxylase large chain HAMAP-Rule MF_01338
PRO_0000251450

Sites

Active site1831Proton acceptor By similarity
Active site3011Proton acceptor By similarity
Metal binding2091Magnesium; via carbamate group By similarity
Metal binding2111Magnesium By similarity
Metal binding2121Magnesium By similarity
Binding site1311Substrate; in homodimeric partner By similarity
Binding site1811Substrate By similarity
Binding site1851Substrate By similarity
Binding site3021Substrate By similarity
Binding site3341Substrate By similarity
Binding site3861Substrate By similarity
Site3411Transition state stabilizer By similarity

Amino acid modifications

Modified residue2091N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3JE87 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: FCE4DF553EB57229

FASTA49254,381
        10         20         30         40         50         60 
MGKSETIAEG KDRYQAGVIP YKKMGYWEPD YQPKDTDIIA MFRITPQPGV DPEEAAAAVA 

        70         80         90        100        110        120 
GESSTATWTV VWTDRLTDCE LYRAKAYRAD LVPNTGEGTK NEAQYFAYIA YDLDLFEPGS 

       130        140        150        160        170        180 
IANLTASIIG NVFGFKAVKA LRLEDMRIPV AYLKTFQGPA TGVVVERERL DKFGRPLLGA 

       190        200        210        220        230        240 
TTKPKLGLSG RNYGRVVYEA LKGGLDFVKD DENINSQPFM HWRDRFLYCM EAVNKASAAT 

       250        260        270        280        290        300 
GEVKGHYLNV TAATMEDMYE RAEFAKSLGS VIIMIDLVVG YTAIQSMAKW ARKNDMILHL 

       310        320        330        340        350        360 
HRAGNSTYSR QKNHGMNFRV ICKWMRMAGV DHIHAGTVVG KLEGDPLMIK GFYDTLLDSH 

       370        380        390        400        410        420 
TPTSLEHGLF FDQDWASLNK VMPVASGGIH AGQMHQLIQY LGEDVILQFG GGTIGHPQGI 

       430        440        450        460        470        480 
QAGAVANRVA LEAMILARNE GRDYVKEGPQ ILQDAAKWCS PLKAALDTWK DVTFNYESTD 

       490 
TADFVPTATA SV 

« Hide

References

[1]"Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707."
Klotz M.G., Arp D.J., Chain P.S.G., El-Sheikh A.F., Hauser L.J., Hommes N.G., Larimer F.W., Malfatti S.A., Norton J.M., Poret-Peterson A.T., Vergez L.M., Ward B.B.
Appl. Environ. Microbiol. 72:6299-6315(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19707 / NCIMB 11848.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000127 Genomic DNA. Translation: ABA56859.1.
RefSeqYP_342389.1. NC_007484.1.

3D structure databases

ProteinModelPortalQ3JE87.
SMRQ3JE87. Positions 13-485.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323261.Noc_0333.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA56859; ABA56859; Noc_0333.
GeneID3706504.
KEGGnoc:Noc_0333.
PATRIC22704376. VBINitOce57959_0432.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1850.
HOGENOMHOG000230831.
KOK01601.
OMACTPLKQA.
OrthoDBEOG6ZKXMS.

Enzyme and pathway databases

BioCycNOCE323261:GCI3-337-MONOMER.

Family and domain databases

Gene3D3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPMF_01338. RuBisCO_L_type1.
InterProIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRBL_NITOC
AccessionPrimary (citable) accession number: Q3JE87
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: November 8, 2005
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families