ID   Q3JCZ0_NITOC            Unreviewed;       331 AA.
AC   Q3JCZ0;
DT   08-NOV-2005, integrated into UniProtKB/TrEMBL.
DT   08-NOV-2005, sequence version 1.
DT   27-MAR-2024, entry version 101.
DE   RecName: Full=Peptide chain release factor 2 {ECO:0000256|HAMAP-Rule:MF_00094};
DE            Short=RF-2 {ECO:0000256|HAMAP-Rule:MF_00094};
GN   Name=prfB {ECO:0000256|HAMAP-Rule:MF_00094};
GN   OrderedLocusNames=Noc_0793 {ECO:0000313|EMBL:ABA57306.1};
OS   Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / JCM 30415 / NCIMB
OS   11848 / C-107).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales; Chromatiaceae;
OC   Nitrosococcus.
OX   NCBI_TaxID=323261 {ECO:0000313|EMBL:ABA57306.1, ECO:0000313|Proteomes:UP000006838};
RN   [1] {ECO:0000313|Proteomes:UP000006838}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107
RC   {ECO:0000313|Proteomes:UP000006838};
RX   PubMed=16957257; DOI=10.1128/AEM.00463-06;
RA   Klotz M.G., Arp D.J., Chain P.S.G., El-Sheikh A.F., Hauser L.J.,
RA   Hommes N.G., Larimer F.W., Malfatti S.A., Norton J.M., Poret-Peterson A.T.,
RA   Vergez L.M., Ward B.B.;
RT   "Complete genome sequence of the marine, chemolithoautotrophic, ammonia-
RT   oxidizing bacterium Nitrosococcus oceani ATCC 19707.";
RL   Appl. Environ. Microbiol. 72:6299-6315(2006).
CC   -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC       translation in response to the peptide chain termination codons UGA and
CC       UAA. {ECO:0000256|HAMAP-Rule:MF_00094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00094}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF2. {ECO:0000256|HAMAP-Rule:MF_00094}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000256|ARBA:ARBA00010835, ECO:0000256|HAMAP-
CC       Rule:MF_00094}.
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DR   EMBL; CP000127; ABA57306.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q3JCZ0; -.
DR   STRING; 323261.Noc_0793; -.
DR   KEGG; noc:Noc_0793; -.
DR   eggNOG; COG1186; Bacteria.
DR   HOGENOM; CLU_036856_6_0_6; -.
DR   InParanoid; Q3JCZ0; -.
DR   Proteomes; UP000006838; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.160.20; -; 1.
DR   Gene3D; 3.30.70.1660; -; 1.
DR   Gene3D; 1.20.58.410; Release factor; 1.
DR   HAMAP; MF_00094; Rel_fac_2; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004374; PrfB.
DR   NCBIfam; TIGR00020; prfB; 1.
DR   PANTHER; PTHR43116; PEPTIDE CHAIN RELEASE FACTOR 2; 1.
DR   PANTHER; PTHR43116:SF3; RF_PROK_I DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; Release factor; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00094};
KW   Methylation {ECO:0000256|ARBA:ARBA00022481, ECO:0000256|HAMAP-
KW   Rule:MF_00094}; Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00094};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006838}.
FT   DOMAIN          211..227
FT                   /note="Prokaryotic-type class I peptide chain release
FT                   factors"
FT                   /evidence="ECO:0000259|PROSITE:PS00745"
FT   COILED          254..281
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   MOD_RES         218
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00094"
SQ   SEQUENCE   331 AA;  37196 MW;  205FC5F0C158A58D CRC64;
     MEVDRELAVP SVWDEPERAQ QLGQERAQLQ SVVAPLAELG GEIIQVRELF ELAMEEGDED
     TAQEIIAELD VVEQRLAKLE FQRMFAGEMD PNNAYLDIQA GSGGTEAQDW ANMLLRMYLR
     WGEKHSFKTE LTEVSEGEVA GIKSAAIRFE GEYAFGWLRT ETGVHRLVRK SPFDSGNRRH
     TSFASVFVYP EVDERIDIEI NPADLRIDTY RASGAGGQHV NRTDSAVRIT HLPSSLVVQC
     QSERSQHQNK AHAMAQLRAK LYELEMRNRQ AEKQAAEEAK ADIGWGSQIR NYVLDQSRIK
     DLRTGIEVGN TQGVLDGDLD SFIEASLKSG F
//