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Q3JCX7

- GLND_NITOC

UniProt

Q3JCX7 - GLND_NITOC

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciNOCE323261:GCI3-813-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:Noc_0806
OrganismiNitrosococcus oceani (strain ATCC 19707 / NCIMB 11848)
Taxonomic identifieri323261 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus
ProteomesiUP000006838: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 892892Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000231683Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi323261.Noc_0806.

Structurei

3D structure databases

ProteinModelPortaliQ3JCX7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini486 – 598113HDUniRule annotationAdd
BLAST
Domaini711 – 78676ACT 1UniRule annotationAdd
BLAST
Domaini822 – 89271ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 348348UridylyltransferaseAdd
BLAST
Regioni349 – 710362Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3JCX7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPNFTGNTRP DPTLFEPAAF AKWIDTSEAP LLLLRSFLKE GIENLKQRFL
60 70 80 90 100
TGTSAAELLP LHAWLVDQIL VQACRLHTKK CPERVALVAV GGYGRGTLHP
110 120 130 140 150
YSDIDILLLL TEETDSILQN SIGHFISFLW DTGLEMGHSV RTVAECQQAA
160 170 180 190 200
RDDLSFITSL MEARLLFGPE LLFKNLQTAI APEQIWNSRQ FFLAKQAEQI
210 220 230 240 250
TRHHKYHDTA YNLEPNLKEG PGGLRDIQMI QWVSKRYFNT WSFDSLLQHD
260 270 280 290 300
FLTVSERKEL LESQSFLWQL RYGLHTLTGR REDRLLFDHQ IALAKQLGYH
310 320 330 340 350
DQGPHLAVEQ LMKDYYRTAE NTGRLNEMLL QLLEERILLV DAKVHVRPIN
360 370 380 390 400
ERFQARNGFL EVINPSVFKH TPSALLEVFL LLQQHPEIKG VRASTIRLIR
410 420 430 440 450
EHRHLIDDNF RADSFNRALF MEIMRQSRGI TRELRRMNKY GILGAYLPVF
460 470 480 490 500
GKIVGQMQYD MFHAYTVDEH TLFLIHNLRR FALPKYAQEL PLCSEIFQRI
510 520 530 540 550
SKPELLYLAG LFHDIAKGRG GDHSKLGAKD ALRFCLYHGL SRDDSRLVAW
560 570 580 590 600
LVAHHLLMSM TAQRRDINDP KVIQRFAREV GDERRLNHLY LLTAADIRAT
610 620 630 640 650
NPNLWNSWKD ALLNKLYTAT QQALRQGLEY PVDKKEHIRD IQNEARQALL
660 670 680 690 700
KDGWTEQKLD ILWSQIDADY FLRHTPNEIR WHIKALAQKE PHDGAPRILV
710 720 730 740 750
RIHNQEPGTM EVFIYARAHA LIFTVTTRTM AQLDLDVLDA RIITTGHGFV
760 770 780 790 800
LESFVVREAA TIRAEADLEF RLQEIQEVLT QRLTQPDRAP PYRPGFIPRK
810 820 830 840 850
LKLFKFPTTI TFTKDRRNQC TVMELTTNNW PGLLSRVCRA LASCQVRLVN
860 870 880 890
AKITTLGTQV VDVFFICNQQ DKPLTPEQQQ QLKEAIYTYL ER
Length:892
Mass (Da):103,332
Last modified:November 8, 2005 - v1
Checksum:i01DF936D6F5B286B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000127 Genomic DNA. Translation: ABA57319.1.
RefSeqiWP_002811130.1. NC_007484.1.
YP_342849.1. NC_007484.1.

Genome annotation databases

EnsemblBacteriaiABA57319; ABA57319; Noc_0806.
GeneIDi3707072.
KEGGinoc:Noc_0806.
PATRICi22705462. VBINitOce57959_0972.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000127 Genomic DNA. Translation: ABA57319.1 .
RefSeqi WP_002811130.1. NC_007484.1.
YP_342849.1. NC_007484.1.

3D structure databases

ProteinModelPortali Q3JCX7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 323261.Noc_0806.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABA57319 ; ABA57319 ; Noc_0806 .
GeneIDi 3707072.
KEGGi noc:Noc_0806.
PATRICi 22705462. VBINitOce57959_0972.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci NOCE323261:GCI3-813-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707."
    Klotz M.G., Arp D.J., Chain P.S.G., El-Sheikh A.F., Hauser L.J., Hommes N.G., Larimer F.W., Malfatti S.A., Norton J.M., Poret-Peterson A.T., Vergez L.M., Ward B.B.
    Appl. Environ. Microbiol. 72:6299-6315(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19707 / NCIMB 11848.

Entry informationi

Entry nameiGLND_NITOC
AccessioniPrimary (citable) accession number: Q3JCX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 8, 2005
Last modified: November 26, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3