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Q3JCX7

- GLND_NITOC

UniProt

Q3JCX7 - GLND_NITOC

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciNOCE323261:GCI3-813-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:Noc_0806
    OrganismiNitrosococcus oceani (strain ATCC 19707 / NCIMB 11848)
    Taxonomic identifieri323261 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus
    ProteomesiUP000006838: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 892892Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000231683Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi323261.Noc_0806.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3JCX7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini486 – 598113HDUniRule annotationAdd
    BLAST
    Domaini711 – 78676ACT 1UniRule annotationAdd
    BLAST
    Domaini822 – 89271ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 348348UridylyltransferaseAdd
    BLAST
    Regioni349 – 710362Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261778.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3JCX7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPNFTGNTRP DPTLFEPAAF AKWIDTSEAP LLLLRSFLKE GIENLKQRFL    50
    TGTSAAELLP LHAWLVDQIL VQACRLHTKK CPERVALVAV GGYGRGTLHP 100
    YSDIDILLLL TEETDSILQN SIGHFISFLW DTGLEMGHSV RTVAECQQAA 150
    RDDLSFITSL MEARLLFGPE LLFKNLQTAI APEQIWNSRQ FFLAKQAEQI 200
    TRHHKYHDTA YNLEPNLKEG PGGLRDIQMI QWVSKRYFNT WSFDSLLQHD 250
    FLTVSERKEL LESQSFLWQL RYGLHTLTGR REDRLLFDHQ IALAKQLGYH 300
    DQGPHLAVEQ LMKDYYRTAE NTGRLNEMLL QLLEERILLV DAKVHVRPIN 350
    ERFQARNGFL EVINPSVFKH TPSALLEVFL LLQQHPEIKG VRASTIRLIR 400
    EHRHLIDDNF RADSFNRALF MEIMRQSRGI TRELRRMNKY GILGAYLPVF 450
    GKIVGQMQYD MFHAYTVDEH TLFLIHNLRR FALPKYAQEL PLCSEIFQRI 500
    SKPELLYLAG LFHDIAKGRG GDHSKLGAKD ALRFCLYHGL SRDDSRLVAW 550
    LVAHHLLMSM TAQRRDINDP KVIQRFAREV GDERRLNHLY LLTAADIRAT 600
    NPNLWNSWKD ALLNKLYTAT QQALRQGLEY PVDKKEHIRD IQNEARQALL 650
    KDGWTEQKLD ILWSQIDADY FLRHTPNEIR WHIKALAQKE PHDGAPRILV 700
    RIHNQEPGTM EVFIYARAHA LIFTVTTRTM AQLDLDVLDA RIITTGHGFV 750
    LESFVVREAA TIRAEADLEF RLQEIQEVLT QRLTQPDRAP PYRPGFIPRK 800
    LKLFKFPTTI TFTKDRRNQC TVMELTTNNW PGLLSRVCRA LASCQVRLVN 850
    AKITTLGTQV VDVFFICNQQ DKPLTPEQQQ QLKEAIYTYL ER 892
    Length:892
    Mass (Da):103,332
    Last modified:November 8, 2005 - v1
    Checksum:i01DF936D6F5B286B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000127 Genomic DNA. Translation: ABA57319.1.
    RefSeqiYP_342849.1. NC_007484.1.

    Genome annotation databases

    EnsemblBacteriaiABA57319; ABA57319; Noc_0806.
    GeneIDi3707072.
    KEGGinoc:Noc_0806.
    PATRICi22705462. VBINitOce57959_0972.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000127 Genomic DNA. Translation: ABA57319.1 .
    RefSeqi YP_342849.1. NC_007484.1.

    3D structure databases

    ProteinModelPortali Q3JCX7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323261.Noc_0806.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA57319 ; ABA57319 ; Noc_0806 .
    GeneIDi 3707072.
    KEGGi noc:Noc_0806.
    PATRICi 22705462. VBINitOce57959_0972.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261778.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci NOCE323261:GCI3-813-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707."
      Klotz M.G., Arp D.J., Chain P.S.G., El-Sheikh A.F., Hauser L.J., Hommes N.G., Larimer F.W., Malfatti S.A., Norton J.M., Poret-Peterson A.T., Vergez L.M., Ward B.B.
      Appl. Environ. Microbiol. 72:6299-6315(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 19707 / NCIMB 11848.

    Entry informationi

    Entry nameiGLND_NITOC
    AccessioniPrimary (citable) accession number: Q3JCX7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3