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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68S-adenosyl-L-methionineUniRule annotation1
Binding sitei93S-adenosyl-L-methionineUniRule annotation1
Binding sitei120S-adenosyl-L-methionineUniRule annotation1
Active sitei143By similarity1
Binding sitei143S-adenosyl-L-methionineUniRule annotation1
Binding sitei147SubstrateUniRule annotation1
Binding sitei179SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Noc_1026
OrganismiNitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107)
Taxonomic identifieri323261 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus
Proteomesi
  • UP000006838 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002291791 – 236tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST236

Interactioni

Protein-protein interaction databases

STRINGi323261.Noc_1026.

Structurei

3D structure databases

ProteinModelPortaliQ3JCB2.
SMRiQ3JCB2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 215Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
KOiK03439.
OMAiPDPWHKS.
OrthoDBiPOG091H015P.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3JCB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNSITLEER GESFQPRPIT SFVRREGRMT PAQKKALEHL WPRYGIDLGT
60 70 80 90 100
GPLNLAAIFN RQAERILEIG FGNGESLLQQ ARAAPERDFL GIEVYRPGIG
110 120 130 140 150
HLLLRLKAEG LENIRVIHGD AWEVLQRALP NPSLDGVQIF FPDPWPKKRH
160 170 180 190 200
HKRRLIQPSF VDLLERKIKP GGWFHLATDW QDYAEQIKAV LSQHAGFNQL
210 220 230
TNEGQSTQRP RTKFEARGQQ QGHGVWDLRF KRSVDS
Length:236
Mass (Da):27,054
Last modified:November 8, 2005 - v1
Checksum:i46D95E814617B9EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000127 Genomic DNA. Translation: ABA57534.1.
RefSeqiWP_002809644.1. NC_007484.1.

Genome annotation databases

EnsemblBacteriaiABA57534; ABA57534; Noc_1026.
KEGGinoc:Noc_1026.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTRMB_NITOC
AccessioniPrimary (citable) accession number: Q3JCB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 8, 2005
Last modified: June 7, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families