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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathway:ilipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA), Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (Noc_1752)
  2. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciNOCE323261:GCI3-1782-MONOMER.
UniPathwayiUPA00359; UER00481.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:Noc_1753
OrganismiNitrosococcus oceani (strain ATCC 19707 / NCIMB 11848)
Taxonomic identifieri323261 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus
ProteomesiUP000006838 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Lipid-A-disaccharide synthasePRO_0000255203Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi323261.Noc_1753.

Structurei

3D structure databases

ProteinModelPortaliQ3JAC1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018004.
KOiK00748.
OMAiIARMLVN.
OrthoDBiEOG6FBWZR.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3JAC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENSAPLVAI VAGEASGDQH AAHLIREVKK IAPGVRFGGI AGPQMRAAGV
60 70 80 90 100
EPLFDSSRLA VVGLVEVLSH LNEIYGAMQK MRHFLEEKHP DLLILVDYPE
110 120 130 140 150
FNLRLAKRAK TLGIKVLYYI SPQVWAWRQY RVHQIGQVVD MMAVVLPFEV
160 170 180 190 200
PFYEQAGVPV NFVGHPLQHE VKSKFNRNEA VVEFGFNPCC KTLGLLPGSR
210 220 230 240 250
HSEIKRLLPV LLEAAERIYS EEPEIQYLLP LAATLKEIDL APYLKGYRLP
260 270 280 290 300
LRVIPDRSYD VMAACDAMVA ASGTVTLEAA LMGVPLVVIY KMNSLSYWMG
310 320 330 340 350
RLLIKVDHIA LCNIIAGEGV APELIQQDAS PERIALEALN LLRDKERRQT
360 370 380
MQQKFYAIKH KLGAGAQRTI AELTVAMLEG ENLGRAS
Length:387
Mass (Da):43,005
Last modified:November 8, 2005 - v1
Checksum:i67CD25C95187726C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000127 Genomic DNA. Translation: ABA58225.1.
RefSeqiWP_002808842.1. NC_007484.1.

Genome annotation databases

EnsemblBacteriaiABA58225; ABA58225; Noc_1753.
KEGGinoc:Noc_1753.
PATRICi22707718. VBINitOce57959_2078.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000127 Genomic DNA. Translation: ABA58225.1.
RefSeqiWP_002808842.1. NC_007484.1.

3D structure databases

ProteinModelPortaliQ3JAC1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323261.Noc_1753.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA58225; ABA58225; Noc_1753.
KEGGinoc:Noc_1753.
PATRICi22707718. VBINitOce57959_2078.

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018004.
KOiK00748.
OMAiIARMLVN.
OrthoDBiEOG6FBWZR.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00481.
BioCyciNOCE323261:GCI3-1782-MONOMER.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707."
    Klotz M.G., Arp D.J., Chain P.S.G., El-Sheikh A.F., Hauser L.J., Hommes N.G., Larimer F.W., Malfatti S.A., Norton J.M., Poret-Peterson A.T., Vergez L.M., Ward B.B.
    Appl. Environ. Microbiol. 72:6299-6315(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19707 / NCIMB 11848.

Entry informationi

Entry nameiLPXB_NITOC
AccessioniPrimary (citable) accession number: Q3JAC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 8, 2005
Last modified: July 22, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.