Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q3J953 (NAGZ_NITOC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:nagZ
Ordered Locus Names:Noc_2183
OrganismNitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) [Complete proteome] [HAMAP]
Taxonomic identifier323261 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides By similarity. HAMAP-Rule MF_00364

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364

Pathway

Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00364.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Beta-hexosaminidase HAMAP-Rule MF_00364
PRO_0000234918

Regions

Region168 – 1692Substrate binding By similarity

Sites

Active site1811Proton donor/acceptor By similarity
Active site2521Nucleophile By similarity
Binding site641Substrate By similarity
Binding site721Substrate By similarity
Binding site1381Substrate By similarity
Site1791Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3J953 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 77F833A74F915E20

FASTA34137,327
        10         20         30         40         50         60 
MTLGPLMIDL KGVTLDSEER AWLGHSWVGG VILFSRNYES PEQIAALIKA VHALKEPRLL 

        70         80         90        100        110        120 
VAVDHEGGRV QRFRDGFTLL PPASLLGKLY DRDQRQAQRL AMITGWLMAV ELRAVGVDFS 

       130        140        150        160        170        180 
FSPVLDLDRG VSAVIGDRAF HGEPDAVIAL ARAYIRGMAW AGMAAVGKHF PGHGSVVADS 

       190        200        210        220        230        240 
HTALPVDKRS WRQIREGDLL PFEHLARAGL PAVMPAHVRY ASVDSRPVGF SPFWLETVLR 

       250        260        270        280        290        300 
GQLGFQGAII SDDLSMAGAA EVGDMSARMQ AALSAGCDMA LVCNDPDGYA LLLDELKGDP 

       310        320        330        340 
YPQTSPRLLR LYGRYPLTRS QLYHNPAWQR AVQMLAAYGE F 

« Hide

References

[1]"Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707."
Klotz M.G., Arp D.J., Chain P.S.G., El-Sheikh A.F., Hauser L.J., Hommes N.G., Larimer F.W., Malfatti S.A., Norton J.M., Poret-Peterson A.T., Vergez L.M., Ward B.B.
Appl. Environ. Microbiol. 72:6299-6315(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19707 / NCIMB 11848.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000127 Genomic DNA. Translation: ABA58643.1.
RefSeqYP_344173.1. NC_007484.1.

3D structure databases

ProteinModelPortalQ3J953.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323261.Noc_2183.

Protein family/group databases

CAZyGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA58643; ABA58643; Noc_2183.
GeneID3705219.
KEGGnoc:Noc_2183.
PATRIC22708720. VBINitOce57959_2574.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000248526.
KOK01207.
OMAAHDIDLS.
OrthoDBEOG6BCT06.

Enzyme and pathway databases

BioCycNOCE323261:GCI3-2217-MONOMER.
UniPathwayUPA00544.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
HAMAPMF_00364. NagZ.
InterProIPR022956. Beta_hexosaminidase_bac.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNAGZ_NITOC
AccessionPrimary (citable) accession number: Q3J953
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 8, 2005
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries