Q3J8Y8 (SYE2_NITOC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	Noc_2250

Organism

Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) [Complete proteome] [HAMAP]

Taxonomic identifier

323261 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Chromatiaceae › Nitrosococcus

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	Glutamate--tRNA ligase 2 HAMAP MF_00022_B		PRO_0000237378
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00022_B
	Motif	253 – 257	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	256	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3J8Y8 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 941A192C5DC60BF5	FASTA	472	52,824
	10 20 30 40 50 60 MNKTALKTRF APSPSGLLHL GNIRTALFNA LLARRSRGLF LLRIEDTDQE RSSEEYVTAL 70 80 90 100 110 120 MEDLRWLALE WQEGPEVEGE AGPYRQSQRR SVYQADFQRL EAEKLAYPCF CSQEELERVR 130 140 150 160 170 180 KRQLAAGQAP RYPGTCARLS PEEVEGKLAA GFKSALRFRV PSLTTIEFED LVRGPQRFAT 190 200 210 220 230 240 GDIGDFIIRR TDGSPAFFFS NALDDALMGV THVLRGEDHL TNTPRQILLL RALGLPIPRY 250 260 270 280 290 300 GHIAMIVGRD GAPLSKRHGS RSVRELREAG YLPEALCNYL ARLGHHYEDS GFLDLDTLAA 310 320 330 340 350 360 QFDLARLGRA PARFDPQQLH HWQREALARC DLDTLERWLA PVAASQVPAD KYQDFIETVR 370 380 390 400 410 420 PNVVLPEDAL HWAKVLFSEE LVLKDGILPI IHEAGAQFFT QALAAVDGSG TDFKALAAQL 430 440 450 460 470 KQTTGAKGRS LFLPLRAAFT GELDGPELAR LLPLMGVARV RQRLQNCVDQ SY

References

[1]

"Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707." Klotz M.G., Arp D.J., Chain P.S.G., El-Sheikh A.F., Hauser L.J., Hommes N.G., Larimer F.W., Malfatti S.A., Norton J.M., Poret-Peterson A.T., Vergez L.M., Ward B.B. Appl. Environ. Microbiol. 72:6299-6315(2006) [PubMed: 16957257] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19707 / NCIMB 11848.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000127 Genomic DNA. Translation: ABA58708.1.
RefSeq	YP_344238.1. NC_007484.1.
3D structure databases
ProteinModelPortal	Q3J8Y8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3J8Y8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3705412.
GenomeReviews	Gene locus Noc_2250 in contig CP000127_GR.
KEGG	noc:Noc_2250.
NMPDR	fig|323261.3.peg.1283.
PATRIC	22708882. VBINitOce57959_2650.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	WQEGPEV.
PhylomeDB	Q3J8Y8.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	NOCE323261:NOC_2250-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_NITOC

Accession

Primary (citable) accession number: Q3J8Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program