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Q3J8W1

- SYI_NITOC

UniProt

Q3J8W1 - SYI_NITOC

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
    Binding sitei608 – 6081ATPUniRule annotation
    Metal bindingi903 – 9031ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi923 – 9231ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciNOCE323261:GCI3-2316-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Noc_2277
    OrganismiNitrosococcus oceani (strain ATCC 19707 / NCIMB 11848)
    Taxonomic identifieri323261 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus
    ProteomesiUP000006838: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 940940Isoleucine--tRNA ligasePRO_1000022094Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi323261.Noc_2277.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3J8W1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi605 – 6095"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3J8W1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKYKETLNL PKTAFPMKAN LAQREPQALK RWQAMDLYGK LRNAARGRPR    50
    FILHDGPPYA NGAIHIGHAV NKILKDIIVK SKSLSGFDAP YVPGWDCHGL 100
    PIELNVEKKV GKPGRKIDVA SFRRACRDYA RKQVNAQRED FERLGVLGDW 150
    CHPYLTMDYQ FEADVIRTLG KIIEKGHLHK GVKPVHWCVD CGSALAEAEV 200
    EYQEKTSPAI DVRFPVVEEV ELLARCKAEI PGAGPMSVVI WTTTPWTLPA 250
    NQAVALHPAL KYVLVECSAG QGRERLLLAE GLYREVLARY SAESAVILAT 300
    CQGDELEGLK LQHPFYERTV PIILGEHVNL EAGTGAVHTA PGHGQDDYVV 350
    GNRYQLSIDN PVGGNGCFLP ETPLFAGEPV FKANDHIIQV LKERGNLLRE 400
    QSLQHSYPHC WRHKTPIIFR ATPQWFINME REGLRTAALA EIKKTRWLPG 450
    WGQQRIEGMV ENRPDWCISR QRAWGTPIPL FVHCQTGELH PDTPRLMEEV 500
    ARRVEEKGIE AWFELEPKEL LGNQAPAYEK VGDTLDVWFD SGVTHTCVLE 550
    TREELGVPAD LYLEGSDQHR GWFQSSLLTS VAIRGTAPYR MVLTHGFTVD 600
    AKGKKMSKSQ GNVVAPQQVM GSLGADILRL WVAATDYRGE MNVSDEILKR 650
    IADSYRRMRN TARYLLANLN GFDPTIHGVP AKDMLALDRW ALDRASLLQG 700
    EIVQAYEDYN FHLIYQRVHN FCAVDMGAIY LDIIKDRQYT TQADSRARRS 750
    AQTAMYHIAE ALVRWLAPVL SFTADEIWQY LPGERGESVF LTTWYEDLPS 800
    LGEEAPWGRA FWDVILAARE AIAKVLEGVR VEGRIGSSLD AEVDLYVEES 850
    LYQALRKLGD ELRFVLITSY ARVYPAQKRP VEALETEMPG LWTVVIPSHY 900
    PKCVRCWHHR EEVGSHEDHP ELCSRCVENT EGGGEQRAFA 940
    Length:940
    Mass (Da):106,115
    Last modified:November 8, 2005 - v1
    Checksum:i487268DA2324ED16
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000127 Genomic DNA. Translation: ABA58735.1.
    RefSeqiYP_344265.1. NC_007484.1.

    Genome annotation databases

    EnsemblBacteriaiABA58735; ABA58735; Noc_2277.
    GeneIDi3705069.
    KEGGinoc:Noc_2277.
    PATRICi22708946. VBINitOce57959_2682.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000127 Genomic DNA. Translation: ABA58735.1 .
    RefSeqi YP_344265.1. NC_007484.1.

    3D structure databases

    ProteinModelPortali Q3J8W1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323261.Noc_2277.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA58735 ; ABA58735 ; Noc_2277 .
    GeneIDi 3705069.
    KEGGi noc:Noc_2277.
    PATRICi 22708946. VBINitOce57959_2682.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci NOCE323261:GCI3-2316-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707."
      Klotz M.G., Arp D.J., Chain P.S.G., El-Sheikh A.F., Hauser L.J., Hommes N.G., Larimer F.W., Malfatti S.A., Norton J.M., Poret-Peterson A.T., Vergez L.M., Ward B.B.
      Appl. Environ. Microbiol. 72:6299-6315(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 19707 / NCIMB 11848.

    Entry informationi

    Entry nameiSYI_NITOC
    AccessioniPrimary (citable) accession number: Q3J8W1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3