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Q3J8W1

- SYI_NITOC

UniProt

Q3J8W1 - SYI_NITOC

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi923 – 9231ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNOCE323261:GCI3-2316-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Noc_2277
OrganismiNitrosococcus oceani (strain ATCC 19707 / NCIMB 11848)
Taxonomic identifieri323261 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus
ProteomesiUP000006838: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligasePRO_1000022094Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323261.Noc_2277.

Structurei

3D structure databases

ProteinModelPortaliQ3J8W1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3J8W1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKYKETLNL PKTAFPMKAN LAQREPQALK RWQAMDLYGK LRNAARGRPR
60 70 80 90 100
FILHDGPPYA NGAIHIGHAV NKILKDIIVK SKSLSGFDAP YVPGWDCHGL
110 120 130 140 150
PIELNVEKKV GKPGRKIDVA SFRRACRDYA RKQVNAQRED FERLGVLGDW
160 170 180 190 200
CHPYLTMDYQ FEADVIRTLG KIIEKGHLHK GVKPVHWCVD CGSALAEAEV
210 220 230 240 250
EYQEKTSPAI DVRFPVVEEV ELLARCKAEI PGAGPMSVVI WTTTPWTLPA
260 270 280 290 300
NQAVALHPAL KYVLVECSAG QGRERLLLAE GLYREVLARY SAESAVILAT
310 320 330 340 350
CQGDELEGLK LQHPFYERTV PIILGEHVNL EAGTGAVHTA PGHGQDDYVV
360 370 380 390 400
GNRYQLSIDN PVGGNGCFLP ETPLFAGEPV FKANDHIIQV LKERGNLLRE
410 420 430 440 450
QSLQHSYPHC WRHKTPIIFR ATPQWFINME REGLRTAALA EIKKTRWLPG
460 470 480 490 500
WGQQRIEGMV ENRPDWCISR QRAWGTPIPL FVHCQTGELH PDTPRLMEEV
510 520 530 540 550
ARRVEEKGIE AWFELEPKEL LGNQAPAYEK VGDTLDVWFD SGVTHTCVLE
560 570 580 590 600
TREELGVPAD LYLEGSDQHR GWFQSSLLTS VAIRGTAPYR MVLTHGFTVD
610 620 630 640 650
AKGKKMSKSQ GNVVAPQQVM GSLGADILRL WVAATDYRGE MNVSDEILKR
660 670 680 690 700
IADSYRRMRN TARYLLANLN GFDPTIHGVP AKDMLALDRW ALDRASLLQG
710 720 730 740 750
EIVQAYEDYN FHLIYQRVHN FCAVDMGAIY LDIIKDRQYT TQADSRARRS
760 770 780 790 800
AQTAMYHIAE ALVRWLAPVL SFTADEIWQY LPGERGESVF LTTWYEDLPS
810 820 830 840 850
LGEEAPWGRA FWDVILAARE AIAKVLEGVR VEGRIGSSLD AEVDLYVEES
860 870 880 890 900
LYQALRKLGD ELRFVLITSY ARVYPAQKRP VEALETEMPG LWTVVIPSHY
910 920 930 940
PKCVRCWHHR EEVGSHEDHP ELCSRCVENT EGGGEQRAFA
Length:940
Mass (Da):106,115
Last modified:November 8, 2005 - v1
Checksum:i487268DA2324ED16
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000127 Genomic DNA. Translation: ABA58735.1.
RefSeqiYP_344265.1. NC_007484.1.

Genome annotation databases

EnsemblBacteriaiABA58735; ABA58735; Noc_2277.
GeneIDi3705069.
KEGGinoc:Noc_2277.
PATRICi22708946. VBINitOce57959_2682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000127 Genomic DNA. Translation: ABA58735.1 .
RefSeqi YP_344265.1. NC_007484.1.

3D structure databases

ProteinModelPortali Q3J8W1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 323261.Noc_2277.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABA58735 ; ABA58735 ; Noc_2277 .
GeneIDi 3705069.
KEGGi noc:Noc_2277.
PATRICi 22708946. VBINitOce57959_2682.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci NOCE323261:GCI3-2316-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707."
    Klotz M.G., Arp D.J., Chain P.S.G., El-Sheikh A.F., Hauser L.J., Hommes N.G., Larimer F.W., Malfatti S.A., Norton J.M., Poret-Peterson A.T., Vergez L.M., Ward B.B.
    Appl. Environ. Microbiol. 72:6299-6315(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19707 / NCIMB 11848.

Entry informationi

Entry nameiSYI_NITOC
AccessioniPrimary (citable) accession number: Q3J8W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: October 29, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3