Q3J7T1 (PROA_NITOC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Noc_2662

Organism

Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) [Complete proteome] [HAMAP]

Taxonomic identifier

323261 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Chromatiaceae › Nitrosococcus

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000230007

Sequences

	Sequence		Length	Mass (Da)
	Q3J7T1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: BA5710F927A2CF58	FASTA	418	45,326
	10 20 30 40 50 60 MDIKDYMQNL GCRARKVSRV LARAGAEEKN QALEMAASFI EKESQALVEA NHKDLAAGRS 70 80 90 100 110 120 KGLDEALLDR LTLTEARIAV MAEGLRQIAS LPDPVGAISE LSYRPSGIQV GRMRVPLGVI 130 140 150 160 170 180 GIIYESRPNV TADAAGLCLK SGNAVILRGG SEAFHSNQAI ACCIHQGLEQ AGLPQEAVQV 190 200 210 220 230 240 VETTDRLAVG ALITQEEHVD VIIPRGGRSL IERISQEAKI PVIKHLDGIC HVYIDDRADL 250 260 270 280 290 300 DKAVAIAVNA KCQRYGVCNA METLLVAREI AAEILPMMGE HYCSAGVELR GCPETQGLLP 310 320 330 340 350 360 HIEPATEEDW YTEYLAPLLA IRIVAGLDEA IEHITHYGSH HTETIVTEDF TRARRFLTEV 370 380 390 400 410 DSSSVMVNAS TRFADGFEYG LGAEIGISTD KLHARGPVGL EGLTSQKWIV LGNGHIRE

References

[1]

"Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707." Klotz M.G., Arp D.J., Chain P.S.G., El-Sheikh A.F., Hauser L.J., Hommes N.G., Larimer F.W., Malfatti S.A., Norton J.M., Poret-Peterson A.T., Vergez L.M., Ward B.B. Appl. Environ. Microbiol. 72:6299-6315(2006) [PubMed: 16957257] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19707 / NCIMB 11848.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000127 Genomic DNA. Translation: ABA59115.1.
RefSeq	YP_344645.1. NC_007484.1.
3D structure databases
ProteinModelPortal	Q3J7T1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3J7T1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3705159.
GenomeReviews	Gene locus Noc_2662 in contig CP000127_GR.
KEGG	noc:Noc_2662.
NMPDR	fig|323261.3.peg.2526.
PATRIC	22709790. VBINitOce57959_3097.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
OMA	YGICGAM.
PhylomeDB	Q3J7T1.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	NOCE323261:NOC_2662-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_NITOC

Accession

Primary (citable) accession number: Q3J7T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program