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Q3J7S8 (SYL_NITOC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Noc_2665
OrganismNitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) [Complete proteome] [HAMAP]
Taxonomic identifier323261 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus

Protein attributes

Sequence length819 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 819819Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009381

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3J7S8 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: E35A47C5CB8B559F

FASTA81992,714
        10         20         30         40         50         60 
MDEHYQAIQI EAEAQAYWEQ HQSFRASEDP SKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMRG RNVLQPMGWD AFGLPAENAA IQNRVPPAQW TYENIDHMRR QLKRLGFAYD 

       130        140        150        160        170        180 
WSRELATCQP DYYRWEQWLF TKLFAKGLVY KKTALVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALVERREIPQ WFLKITAYGE ELLTALDGLT GWPEQVRAMQ RNWIGRSEGI EVQFHVEGKE 

       250        260        270        280        290        300 
ALAVFTTRPD TLMGVTFVSV AAEHPLAREA ATSNPALAAF LEQCQRTATS EAILETLEKE 

       310        320        330        340        350        360 
GMDTGFKAIH PITGEAVPIW VANFVLMGYG TGAVMAVPAH DQRDYEFAKV YGLPIQQVIA 

       370        380        390        400        410        420 
PKDDQASCNL EQSAFVEKGL LINSGDFTGL DFDQAFAAIA EHLERGGKGQ RRVNYRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPIVHCLHC GAIPVPEEDL PVVLPERVRF DGIRSPLKQL PEFYQTSCPQ 

       490        500        510        520        530        540 
CGGQAKRETD TFDTFFESSW YYARYCCPDN NATMVDERGD YWLPVDQYIG GIEHAVLHLL 

       550        560        570        580        590        600 
YARFFHKVMR DMGLVRSDEP FTHLLTQGMV LKDGAKMSKS KGNTVDPQAL IEHYGADTAR 

       610        620        630        640        650        660 
LFIMFAAPPE QSLEWSESGV EGAHRFLKRL WRIVAAHVEQ DPKAALTLKV KDLNSEQKSF 

       670        680        690        700        710        720 
RAKVHETIAK ASDDIGRRYT FNTAIAAIME LMNALAKFDD SSPQGQAIRQ EALEAVVLLL 

       730        740        750        760        770        780 
SPITPHICHR LWQELGHREA IIGVPWPEAD PDALAKESIE LVVQVNGKRR GQITVAVQAP 

       790        800        810 
REEIEGQAQA EPNVQRFIEG KTVQKVIIVP NRLVNLVVS 

« Hide

References

[1]"Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707."
Klotz M.G., Arp D.J., Chain P.S.G., El-Sheikh A.F., Hauser L.J., Hommes N.G., Larimer F.W., Malfatti S.A., Norton J.M., Poret-Peterson A.T., Vergez L.M., Ward B.B.
Appl. Environ. Microbiol. 72:6299-6315(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19707 / NCIMB 11848.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000127 Genomic DNA. Translation: ABA59118.1.
RefSeqYP_344648.1. NC_007484.1.

3D structure databases

ProteinModelPortalQ3J7S8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323261.Noc_2665.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA59118; ABA59118; Noc_2665.
GeneID3705162.
KEGGnoc:Noc_2665.
PATRIC22709796. VBINitOce57959_3100.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycNOCE323261:GCI3-2712-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_NITOC
AccessionPrimary (citable) accession number: Q3J7S8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries