Reviewed, UniProtKB/Swiss-Prot Q3J7A0 (ARGJ_NITOC)

Names and origin

Protein names

Recommended name:
    Arginine biosynthesis bifunctional protein argJ
Cleaved into the following 2 chains:
    1- Recommended name:
            Arginine biosynthesis bifunctional protein argJ alpha chain
    2- Recommended name:
            Arginine biosynthesis bifunctional protein argJ beta chain
Including the following 2 domains:
    1- Recommended name:
            Glutamate N-acetyltransferase
              EC=2.3.1.35
        Alternative name(s):
            Ornithine acetyltransferase
              Short name=OATase
            Ornithine transacetylase
    2- Recommended name:
            Amino-acid acetyltransferase
              EC=2.3.1.1
        Alternative name(s):
            N-acetylglutamate synthase
              Short name=AGS

Gene names

Name:	argJ
Ordered Locus Names:	Noc_2850

Organism

Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) [Complete proteome] [HAMAP]

Taxonomic identifier

323261 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Chromatiaceae › Nitrosococcus

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate By similarity.
Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP MF_01106 Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01106
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP MF_01106 Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm Probable.
Miscellaneous	Some bacteria possess a monofunctional argJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP MF_01106
Sequence similarities	Belongs to the argJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino-acid N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	Arginine biosynthesis bifunctional protein argJ alpha chain By similarity		PRO_0000227238
	Chain	190 – 405	216	Arginine biosynthesis bifunctional protein argJ beta chain By similarity		PRO_0000227239
Sites
	Site	189 – 190	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3J7A0-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: F0E494143E1C816C	FASTA	405	42,325
	10 20 30 40 50 60 MAAGLEHLQE LLPVAGVELG TVAAGIRYRD RPDLAIIAVT GGSTCAAVFT RNAFCAAPVI 70 80 90 100 110 120 VARRHLAGNS PRFLLINAGN ANAGTGIQGI VDAETCCATL AELGGVASTA ILPFSTGVIG 130 140 150 160 170 180 ELLPVSRIQA ALPQVWQVCS PTGWSSVARA IMTTDTVPKG TSVHFTCQGH PVTVTGIAKG 190 200 210 220 230 240 SGMIRPDMAT MLAFLATDAQ VPKKVLQRCL ARGVAESFNR ITVDGDTSTN DACVLLATGQ 250 260 270 280 290 300 SEVSIATIED PAFAALQQAV TEVCLSLAQA IVRDGEGATK FITVEVAGGR NIQECEQVAY 310 320 330 340 350 360 TIAQSPLVKT ACFASDPNWG RILAAIGRSG VPDLEITGVS LYLGEACVIH GGVLHPEYTE 370 380 390 400 EQGRTVMAQD DITLAVELGR GDARVQIWTC DLSFAYVKIN AEYRS

References

[1]

"Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707." Klotz M.G., Arp D.J., Chain P.S.G., El-Sheikh A.F., Hauser L.J., Hommes N.G., Larimer F.W., Malfatti S.A., Norton J.M., Poret-Peterson A.T., Vergez L.M., Ward B.B. Appl. Environ. Microbiol. 72:6299-6315(2006) [PubMed: 16957257] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000127 Genomic DNA. Translation: ABA59296.1.
RefSeq	YP_344826.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3J7A0.
Protein family/group databases
MEROPS	T05.001.
Genome annotation databases
GeneID	3705391.
GenomeReviews	Gene locus Noc_2850 in contig CP000127_GR.
KEGG	noc:Noc_2850.
NMPDR	fig|323261.3.peg.1632.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3J7A0.
OMA	IVNSGNA.
Enzyme and pathway databases
BioCyc	NOCE323261:NOC_2850-MON.
Family and domain databases
HAMAP	MF_01106. [Tree]
InterPro	IPR002813. Arg_biosynth_ArgJ. [Graphical view]
PANTHER	PTHR23100. ArgJ. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
ProDom	PD004193. ArgJ. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ_NITOC

Accession

Primary (citable) accession number: Q3J7A0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)