Reviewed, UniProtKB/Swiss-Prot Q3J653 (CHEB1_RHOS4)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	RHOS4_01630
ORF Names:	RSP_1588

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [Complete proteome] [HAMAP]

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000225481
Regions
	Domain	11 – 128	118	Response regulatory
	Domain	170 – 363	194	CheB-type methylesterase
Sites
	Active site	183	1	By similarity
	Active site	209	1	By similarity
	Active site	305	1	By similarity
Amino acid modifications
	Modified residue	62	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3J653-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 304F844CA7EB83C7	FASTA	369	39,605
	10 20 30 40 50 60 MTTHAAAPST RVLIVDDSAA ARAMFKVIVE SDPALQVMAA VPDAFAAARA MRTELPDVIL 70 80 90 100 110 120 LDLELPGMDG LTFLRKIMQQ HPIPVVVCSS HVGAGTEAMV SALELGAREV ISKPAARNDL 130 140 150 160 170 180 ERQEASIRIC DAIRAATETT RRRSQPEPRP LAPGPKLTAD EILPARPPRP VPETMPVVCI 190 200 210 220 230 240 GASTGGTEAL RDVLTALPAS APPIVIVQHM PRGFTAAFAR RLDSLCAIEV LEAEDEMQVM 250 260 270 280 290 300 PGRAIIAQGD RHLLLRRRNQ GYRVSVLDGA YVCRHRPSVD VLFRSAAQEA GGNALGVIMT 310 320 330 340 350 360 GMGDDGARCM AEMRAAGAET IAQNEESCVV YGMPREAVAH GGVGKVEPLD RLAARIMEFG RRHTERTVR

References

[1]

"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000143 Genomic DNA. Translation: ABA77731.1.
RefSeq	YP_351632.1.
3D structure databases
SMR	Q3J653. Positions 9-361.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3J653.
Genome annotation databases
GeneID	3718577.
GenomeReviews	Gene locus RHOS4_01630 in contig CP000143_GR.
KEGG	rsp:RSP_1588.
NMPDR	fig|272943.3.peg.384.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	QAPDVIT.
PhylomeDB	Q3J653.
Enzyme and pathway databases
BioCyc	RSPH272943:RSP_1588-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_RHOS4

Accession

Primary (citable) accession number: Q3J653

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 8, 2005
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)