Q3J5X0 (ISPH_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	RHOS4_02460
ORF Names:	RSP_1666

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021171

Sequences

	Sequence		Length	Mass (Da)
	Q3J5X0 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 8AB91ECA78D6FFAE	FASTA	319	34,661
	10 20 30 40 50 60 MTMPPLTLYL AAPRGFCAGV DRAIKIVEMA LEKWGAPVYV RHEIVHNKFV VDRLRDMGAV 70 80 90 100 110 120 FVEELDEAPT DRPVIFSAHG VPKAIPAEAE RRNMVYVDAT CPLVSKVHLE AERHHENGLQ 130 140 150 160 170 180 MIMIGHAGHP ETVGTMGQLP EGEVLLVETV EDVAGLEVRD PERLAYITQT TLSIDDTAAI 190 200 210 220 230 240 VAALRERFPA IAIPRKEDIC YATTNRQGAV KAIAGRIDAL LVIGAPNSSN SKRLVEVGRA 250 260 270 280 290 300 AGCRVAQLVQ RATDIDWEAL QGATAVGVAA GASAPEVLVD EVIAAFRARF DTTVEAVETV 310 KERVEFKVPR ILREPAETP

References

[1]

"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000143 Genomic DNA. Translation: ABA77814.1.
RefSeq	YP_351715.1. NC_007493.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3J5X0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3717932.
GenomeReviews	Gene locus RHOS4_02460 in contig CP000143_GR.
KEGG	rsp:RSP_1666.
NMPDR	fig|272943.3.peg.466.
PATRIC	23150282. VBIRhoSph57909_0543.
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG335228.
OMA	WGAPVYV.
PhylomeDB	Q3J5X0.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	RSPH272943:RSP_1666-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_RHOS4

Accession

Primary (citable) accession number: Q3J5X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program