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Q3J5S4

- EFTU_RHOS4

UniProt

Q3J5S4 - EFTU_RHOS4

Protein

Elongation factor Tu

Gene

tuf1

more
Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 268GTPUniRule annotation
    Nucleotide bindingi76 – 805GTPUniRule annotation
    Nucleotide bindingi131 – 1344GTPUniRule annotation

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: UniProtKB-HAMAP
    3. translation elongation factor activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Elongation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRSPH272943:GJAS-289-MONOMER.
    RSPH272943:GJAS-304-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor TuUniRule annotation
    Short name:
    EF-TuUniRule annotation
    Gene namesi
    Name:tuf1UniRule annotation
    Synonyms:tufA
    Ordered Locus Names:RHOS4_02780
    ORF Names:RSP_1707
    AND
    Name:tuf2UniRule annotation
    Synonyms:tufA
    Ordered Locus Names:RHOS4_02920
    ORF Names:RSP_1714
    OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
    Taxonomic identifieri272943 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
    ProteomesiUP000002703: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 391391Elongation factor TuPRO_0000337489Add
    BLAST

    Proteomic databases

    PRIDEiQ3J5S4.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272943.RSP_1714.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3J5S4.
    SMRiQ3J5S4. Positions 2-391.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 201192tr-type GAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni19 – 268G1By similarity
    Regioni55 – 595G2By similarity
    Regioni76 – 794G3By similarity
    Regioni131 – 1344G4By similarity
    Regioni169 – 1713G5By similarity

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0050.
    HOGENOMiHOG000229290.
    KOiK02358.
    OMAiERKPHIN.
    OrthoDBiEOG6R5C6X.
    PhylomeDBiQ3J5S4.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00118_B. EF_Tu_B.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004541. Transl_elong_EFTu/EF1A_bac/org.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view]
    PfamiPF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3J5S4-1 [UniParc]FASTAAdd to Basket

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    MAKAKFERNK PHVNIGTIGH VDHGKTTLTA AITKYFGEFR AYDQIDGAPE    50
    ERARGITIST AHVEYESDTR HYAHVDCPGH ADYVKNMITG AAQMDGAILV 100
    VNAADGPMPQ TREHILLGRQ VGIPYMVVYM NKVDQVDDPE LIELVEMEIR 150
    ELLSSYDYPG DDIPIIKGSA LAAMNGTDKE IGEDSIRALI AAVDEYIPTP 200
    ARAVDQPFLM PVEDVFSISG RGTVATGRIE RGVVKVGEEL EIVGIRPSKK 250
    TVCTGVEMFR KLLDQGEAGD NVGLLLRGVD RDGIERGQVL CKPGSVKPHT 300
    KFEAEAYILT KEEGGRHTPF FANYRPQFYF RTTDVTGTVQ LPEGTEMVMP 350
    GDNLKFNVEL IAPIAMEEKL RFAIREGGRT VGAGVVSKII A 391
    Length:391
    Mass (Da):42,874
    Last modified:November 8, 2005 - v1
    Checksum:i57CECF288B04BB60
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000143 Genomic DNA. Translation: ABA77846.1.
    CP000143 Genomic DNA. Translation: ABA77860.1.
    RefSeqiYP_351747.1. NC_007493.2.
    YP_351761.1. NC_007493.2.

    Genome annotation databases

    EnsemblBacteriaiABA77846; ABA77846; RSP_1707.
    ABA77860; ABA77860; RSP_1714.
    GeneIDi3718904.
    3718919.
    KEGGirsp:RSP_1707.
    rsp:RSP_1714.
    PATRICi23150348. VBIRhoSph57909_0576.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000143 Genomic DNA. Translation: ABA77846.1 .
    CP000143 Genomic DNA. Translation: ABA77860.1 .
    RefSeqi YP_351747.1. NC_007493.2.
    YP_351761.1. NC_007493.2.

    3D structure databases

    ProteinModelPortali Q3J5S4.
    SMRi Q3J5S4. Positions 2-391.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272943.RSP_1714.

    Proteomic databases

    PRIDEi Q3J5S4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA77846 ; ABA77846 ; RSP_1707 .
    ABA77860 ; ABA77860 ; RSP_1714 .
    GeneIDi 3718904.
    3718919.
    KEGGi rsp:RSP_1707.
    rsp:RSP_1714.
    PATRICi 23150348. VBIRhoSph57909_0576.

    Phylogenomic databases

    eggNOGi COG0050.
    HOGENOMi HOG000229290.
    KOi K02358.
    OMAi ERKPHIN.
    OrthoDBi EOG6R5C6X.
    PhylomeDBi Q3J5S4.

    Enzyme and pathway databases

    BioCyci RSPH272943:GJAS-289-MONOMER.
    RSPH272943:GJAS-304-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_00118_B. EF_Tu_B.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004541. Transl_elong_EFTu/EF1A_bac/org.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view ]
    Pfami PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00485. EF-Tu. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

    Entry informationi

    Entry nameiEFTU_RHOS4
    AccessioniPrimary (citable) accession number: Q3J5S4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3