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Q3J5H6

- GLND_RHOS4

UniProt

Q3J5H6 - GLND_RHOS4

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, RHOS4_03900, RSP_1811
Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [glutamate-ammonia-ligase] adenylyltransferase activity Source: InterPro
  2. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  3. amino acid binding Source: InterPro
  4. metal ion binding Source: InterPro
  5. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-403-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:RHOS4_03900
ORF Names:RSP_1811
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000002703: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 930930Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_0000231690Add
BLAST

Proteomic databases

PRIDEiQ3J5H6.

Interactioni

Protein-protein interaction databases

STRINGi272943.RSP_1811.

Structurei

3D structure databases

ProteinModelPortaliQ3J5H6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini505 – 624120HDAdd
BLAST
Domaini742 – 81877ACT 1Add
BLAST
Domaini852 – 92776ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 387387UridylyltransferaseUniRule annotationAdd
BLAST
Regioni388 – 741354Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiLYCLWDM.
OrthoDBiEOG6CCH44.
PhylomeDBiQ3J5H6.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR005190. GlnE_rpt_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF03710. GlnE. 1 hit.
PF01966. HD. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3J5H6-1 [UniParc]FASTAAdd to Basket

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MAPASEAGPA QDPLFSGLIL PRSRILDAEA LTRSILAEID AAGPLDPKSA    50
RAIAVRQMTE AKAAGNRALS ETFEARPREA RGLIRAQAAL TDGLVTAALR 100
VACERLHPLA NPTEAERIAV LAVGGYGRAE MAPHSDVDLL FLTPWKITPW 150
AEMVIESMLY MLWDLRLKVG HSSRTVKDCL RLGREDITIR TALLEHRFLA 200
GHAPLAAELD ETLWNDLFRG TGAEFIDAKL EERGQRHKRQ GGQRYVLEPN 250
VKEGKGGLRD LQTLYWIGKY LNRVPSPSGL VAAGLLTRDE FETFERAESF 300
LWAVRCHLHY ATGRATDQLT FDLQVEVAAR MGYADTRGRR GVEVFMQDYF 350
RHATRVGELT RVFLAQLEAR HEKREPKIMG LFRRKKRLKP EYSLVNGRID 400
VLDPKAFLAD KLNLLRIFEE ALRTGFLIHP GAMRLIAANL HLIDEEMQHD 450
REANRIFLDM LLRHGNPERA LRRMNELGVL GAFIPEFERI VAMMQFNVYH 500
HYTVDEHTIQ CISTLAQIER HELDEELPIA NRILTDGISR RVIYVALLLH 550
DIGKGRPEDH SILGAQIARR VAPRFGLTAE ECETVEWLVR YHLLMSDMAQ 600
KRDIGDPRTV RDFAKAVRSK KRLDLLTVLT VCDIRGVGPG TWNNWKAQLL 650
RKLYRDTVTA LDAGLESLNR ENRADEAKRA LRELLEEWDP KDLRAELARH 700
YPPYWQALSN ATHAVFARML RGLGETEIRI DLDPDPDRDA TRACFALADH 750
PGIFSRLAGA LALVGANVVD ARTYTTKDGY ATAVFWIQDS EGSPYEISRL 800
PRLTSMIDKT LKGEVVAREA LKDRDKLKKR EAQFRFPTHI AFDNEGSDIY 850
TIIEVDTRDR PGLLYDLTRT LAANNIYIAS AVIATYGAQV VDSFYVKDMF 900
GLKLHQKNRQ ETLEKKLRQA IVEGAERAKQ 930
Length:930
Mass (Da):105,347
Last modified:November 8, 2005 - v1
Checksum:iF2C8D050AD54C166
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000143 Genomic DNA. Translation: ABA77958.1.
RefSeqiYP_351859.1. NC_007493.2.

Genome annotation databases

EnsemblBacteriaiABA77958; ABA77958; RSP_1811.
GeneIDi3719057.
KEGGirsp:RSP_1811.
PATRICi23150588. VBIRhoSph57909_0694.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000143 Genomic DNA. Translation: ABA77958.1 .
RefSeqi YP_351859.1. NC_007493.2.

3D structure databases

ProteinModelPortali Q3J5H6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272943.RSP_1811.

Proteomic databases

PRIDEi Q3J5H6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABA77958 ; ABA77958 ; RSP_1811 .
GeneIDi 3719057.
KEGGi rsp:RSP_1811.
PATRICi 23150588. VBIRhoSph57909_0694.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi LYCLWDM.
OrthoDBi EOG6CCH44.
PhylomeDBi Q3J5H6.

Enzyme and pathway databases

BioCyci RSPH272943:GJAS-403-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR005190. GlnE_rpt_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF03710. GlnE. 1 hit.
PF01966. HD. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Entry informationi

Entry nameiGLND_RHOS4
AccessioniPrimary (citable) accession number: Q3J5H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 8, 2005
Last modified: July 9, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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