Q3J5H6 (GLND_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
[Protein-PII] uridylyltransferase
Short name=PII uridylyl-transferase
EC=2.7.7.59

Alternative name(s):
UTase
Uridylyl-removing enzyme

Gene names

Name:	glnD
Ordered Locus Names:	RHOS4_03900
ORF Names:	RSP_1811

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [Reference proteome] [HAMAP]

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter › Rhodobacter sphaeroides

Protein attributes

Sequence length	930 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies, by uridylylation or deuridylylation the PII (GlnB) regulatory protein By similarity. HAMAP-Rule MF_00277
Catalytic activity	UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP-Rule MF_00277
Sequence similarities	Belongs to the GlnD family.

Ontologies

Keywords
Biological process	Nitrogen fixation
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	[glutamate-ammonia-ligase] adenylyltransferase activity Inferred from electronic annotation. Source: InterPro [protein-PII] uridylyltransferase activity Inferred from electronic annotation. Source: EC amino acid binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: InterPro phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 930	930	[Protein-PII] uridylyltransferase HAMAP-Rule MF_00277		PRO_0000231690

Sequences

	Sequence		Length	Mass (Da)
	Q3J5H6 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: F2C8D050AD54C166	FASTA	930	105,347
	10 20 30 40 50 60 MAPASEAGPA QDPLFSGLIL PRSRILDAEA LTRSILAEID AAGPLDPKSA RAIAVRQMTE 70 80 90 100 110 120 AKAAGNRALS ETFEARPREA RGLIRAQAAL TDGLVTAALR VACERLHPLA NPTEAERIAV 130 140 150 160 170 180 LAVGGYGRAE MAPHSDVDLL FLTPWKITPW AEMVIESMLY MLWDLRLKVG HSSRTVKDCL 190 200 210 220 230 240 RLGREDITIR TALLEHRFLA GHAPLAAELD ETLWNDLFRG TGAEFIDAKL EERGQRHKRQ 250 260 270 280 290 300 GGQRYVLEPN VKEGKGGLRD LQTLYWIGKY LNRVPSPSGL VAAGLLTRDE FETFERAESF 310 320 330 340 350 360 LWAVRCHLHY ATGRATDQLT FDLQVEVAAR MGYADTRGRR GVEVFMQDYF RHATRVGELT 370 380 390 400 410 420 RVFLAQLEAR HEKREPKIMG LFRRKKRLKP EYSLVNGRID VLDPKAFLAD KLNLLRIFEE 430 440 450 460 470 480 ALRTGFLIHP GAMRLIAANL HLIDEEMQHD REANRIFLDM LLRHGNPERA LRRMNELGVL 490 500 510 520 530 540 GAFIPEFERI VAMMQFNVYH HYTVDEHTIQ CISTLAQIER HELDEELPIA NRILTDGISR 550 560 570 580 590 600 RVIYVALLLH DIGKGRPEDH SILGAQIARR VAPRFGLTAE ECETVEWLVR YHLLMSDMAQ 610 620 630 640 650 660 KRDIGDPRTV RDFAKAVRSK KRLDLLTVLT VCDIRGVGPG TWNNWKAQLL RKLYRDTVTA 670 680 690 700 710 720 LDAGLESLNR ENRADEAKRA LRELLEEWDP KDLRAELARH YPPYWQALSN ATHAVFARML 730 740 750 760 770 780 RGLGETEIRI DLDPDPDRDA TRACFALADH PGIFSRLAGA LALVGANVVD ARTYTTKDGY 790 800 810 820 830 840 ATAVFWIQDS EGSPYEISRL PRLTSMIDKT LKGEVVAREA LKDRDKLKKR EAQFRFPTHI 850 860 870 880 890 900 AFDNEGSDIY TIIEVDTRDR PGLLYDLTRT LAANNIYIAS AVIATYGAQV VDSFYVKDMF 910 920 930 GLKLHQKNRQ ETLEKKLRQA IVEGAERAKQ

References

[1]

"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000143 Genomic DNA. Translation: ABA77958.1.
RefSeq	YP_351859.1. NC_007493.1.
3D structure databases
ProteinModelPortal	Q3J5H6.
ModBase	Search...
Protein-protein interaction databases
STRING	272943.RSP_1811.
Proteomic databases
PRIDE	Q3J5H6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3719057.
KEGG	rsp:RSP_1811.
PATRIC	23150588. VBIRhoSph57909_0694.
Phylogenomic databases
eggNOG	COG2844.
HOGENOM	HOG000261779.
KO	K00990.
OMA	SPKVWNA.
ProtClustDB	PRK05092.
Enzyme and pathway databases
BioCyc	RSPH272943:GJAS-403-MONOMER.
Family and domain databases
Gene3D	1.10.3210.10. 1 hit.
HAMAP	MF_00277. PII_uridylyl-transf.
InterPro	IPR002912. ACT_dom. IPR010043. GlnD_Uridyltrans. IPR005190. GlnE_rpt_dom. IPR003607. HD/PDEase_dom. IPR006674. HD_domain. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
PANTHER	PTHR13734:SF1. PTHR13734:SF1. 1 hit.
Pfam	PF01842. ACT. 2 hits. PF08335. GlnD_UR_UTase. 1 hit. PF03710. GlnE. 1 hit. PF01966. HD. 1 hit. [Graphical view]
PIRSF	PIRSF006288. PII_uridyltransf. 1 hit.
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01693. UTase_glnD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLND_RHOS4

Accession

Primary (citable) accession number: Q3J5H6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 8, 2005
Last modified:	May 29, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program