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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (mtnP)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11PhosphateUniRule annotation1
Sitei166Important for substrate specificityUniRule annotation1
Binding sitei184Substrate; via amide nitrogenUniRule annotation1
Binding sitei185PhosphateUniRule annotation1
Sitei221Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Gene namesi
Name:mtnPUniRule annotation
Ordered Locus Names:RHOS4_04180
ORF Names:RSP_1837
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
Proteomesi
  • UP000002703 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004150981 – 290S-methyl-5'-thioadenosine phosphorylaseAdd BLAST290

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi272943.RSP_1837.

Structurei

3D structure databases

ProteinModelPortaliQ3J5E8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 54Phosphate bindingUniRule annotation2
Regioni86 – 87Phosphate bindingUniRule annotation2
Regioni208 – 210Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RHB. Bacteria.
COG0005. LUCA.
HOGENOMiHOG000228986.
KOiK00772.
OMAiCVAHVSM.
OrthoDBiPOG091H0228.
PhylomeDBiQ3J5E8.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP. 1 hit.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3J5E8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTMIGVIGG SGVYEIDGLE DAVWTKVETP WGDPSDEILT GRLDGVPMAF
60 70 80 90 100
LPRHGRGHVH SPTTVPYRAN IDALKRLGVT DLVSVSACGS FREEMAPGDF
110 120 130 140 150
VIVDQFIDRS FARAKSFFGS GCVAHVSLAH PTCGRLSALC AEAARATGVT
160 170 180 190 200
VHEGGTYLCM EGPQFSTLAE SLLYKSWGCH VIGMTNMPEA KLAREAEICY
210 220 230 240 250
ASVAMVTDYD SWHPHHGEVD ITAIIATLGT NADHARGLVA GLPARLGTER
260 270 280 290
DLCPHGCDRA LDHALMTAPA KRDPELLAKL DAVAGRVLKG
Length:290
Mass (Da):30,950
Last modified:November 8, 2005 - v1
Checksum:i35B46FAB615584CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA77986.1.
RefSeqiWP_011337020.1. NZ_AKVW01000001.1.
YP_351887.1. NC_007493.2.

Genome annotation databases

EnsemblBacteriaiABA77986; ABA77986; RSP_1837.
GeneIDi3719104.
KEGGirsp:RSP_1837.
PATRICi23150648. VBIRhoSph57909_0724.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA77986.1.
RefSeqiWP_011337020.1. NZ_AKVW01000001.1.
YP_351887.1. NC_007493.2.

3D structure databases

ProteinModelPortaliQ3J5E8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272943.RSP_1837.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA77986; ABA77986; RSP_1837.
GeneIDi3719104.
KEGGirsp:RSP_1837.
PATRICi23150648. VBIRhoSph57909_0724.

Phylogenomic databases

eggNOGiENOG4107RHB. Bacteria.
COG0005. LUCA.
HOGENOMiHOG000228986.
KOiK00772.
OMAiCVAHVSM.
OrthoDBiPOG091H0228.
PhylomeDBiQ3J5E8.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP. 1 hit.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTAP_RHOS4
AccessioniPrimary (citable) accession number: Q3J5E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.