Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NAD/NADP-dependent betaine aldehyde dehydrogenase

Gene

betB

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid.UniRule annotation

Catalytic activityi

Betaine aldehyde + NAD+ + H2O = betaine + NADH.UniRule annotation

Cofactori

K(+)UniRule annotationNote: Binds 2 potassium ions per subunit.UniRule annotation

Pathway: betaine biosynthesis via choline pathway

This protein is involved in step 1 of the subpathway that synthesizes betaine from betaine aldehyde.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NAD/NADP-dependent betaine aldehyde dehydrogenase (betB)
This subpathway is part of the pathway betaine biosynthesis via choline pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine from betaine aldehyde, the pathway betaine biosynthesis via choline pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi27 – 271Potassium 1; via carbonyl oxygenUniRule annotation
Metal bindingi93 – 931Potassium 1UniRule annotation
Active sitei161 – 1611Charge relay systemUniRule annotation
Metal bindingi179 – 1791Potassium 1; via carbonyl oxygenUniRule annotation
Binding sitei208 – 2081NAD/NADP; via amide nitrogenUniRule annotation
Metal bindingi243 – 2431Potassium 2; via carbonyl oxygenUniRule annotation
Active sitei249 – 2491Proton acceptorUniRule annotation
Binding sitei283 – 2831NAD/NADPUniRule annotation
Binding sitei380 – 3801NAD/NADPUniRule annotation
Metal bindingi450 – 4501Potassium 2; via carbonyl oxygenUniRule annotation
Metal bindingi453 – 4531Potassium 2; via carbonyl oxygenUniRule annotation
Active sitei457 – 4571Charge relay systemUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi149 – 1524NAD/NADPUniRule annotation
Nucleotide bindingi175 – 1784NAD/NADPUniRule annotation
Nucleotide bindingi227 – 2326NAD/NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, NADP, Potassium

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-790-MONOMER.
UniPathwayiUPA00529; UER00386.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD/NADP-dependent betaine aldehyde dehydrogenaseUniRule annotation (EC:1.2.1.8UniRule annotation)
Short name:
BADHUniRule annotation
Gene namesi
Name:betBUniRule annotation
Ordered Locus Names:RHOS4_07670
ORF Names:RSP_2183
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000002703 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483NAD/NADP-dependent betaine aldehyde dehydrogenasePRO_1000047055Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei283 – 2831Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi272943.RSP_2183.

Structurei

3D structure databases

ProteinModelPortaliQ3J4E9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
KOiK00130.
OMAiHVYQNIA.
OrthoDBiEOG6BS8QW.
PhylomeDBiQ3J4E9.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00804. BADH.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01804. BADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3J4E9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAQPAASHF VDGRPLEDET GAPIPVIYPA TGEEIARLHE ATPAVIEAAL
60 70 80 90 100
ASGARAQAAW AAMRPVERAR ILRRASDLIR AHNEELSLLE TLDTGKPLQE
110 120 130 140 150
TLVADWASGA DALEFFAGLA PVVTGETVPL GQDFVYTIRE PLGLCVGIGA
160 170 180 190 200
WNYPSQIACW KAAPALALGN AMVFKPSEVT PLGALKLAEI LIEAGLPPGL
210 220 230 240 250
FNVVQGRGAV GASLVTDSRV AKVSLTGSVP TGRRVYAAAA EGVRHVTMEL
260 270 280 290 300
GGKSPLIVFD DADLESAIGA AMLGNFYSAG QICSNGTRVF VQKGIKEAFL
310 320 330 340 350
ARLAERADAI RMGDPLDPEV QMGPLVSQAQ LEKVLAYIEK ARAEGGRLVC
360 370 380 390 400
GGEASVSPGC YVQPTVFADV TDAMTLAREE VFGPVMAVLD FETEEEAIAR
410 420 430 440 450
ANATDFGLAA GVFTADLTRA HRVVAQLQAG TCWINAYNLT PVEAPFGGVK
460 470 480
LSGVGRENGR AAVQHYTQVK SVYVGMGPVD APY
Length:483
Mass (Da):50,804
Last modified:November 8, 2005 - v1
Checksum:i9BFC75AA2D687748
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA78335.1.
RefSeqiWP_011337293.1. NZ_AKVW01000001.1.
YP_352236.1. NC_007493.2.

Genome annotation databases

EnsemblBacteriaiABA78335; ABA78335; RSP_2183.
GeneIDi3719653.
KEGGirsp:RSP_2183.
PATRICi23151370. VBIRhoSph57909_1079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA78335.1.
RefSeqiWP_011337293.1. NZ_AKVW01000001.1.
YP_352236.1. NC_007493.2.

3D structure databases

ProteinModelPortaliQ3J4E9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272943.RSP_2183.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA78335; ABA78335; RSP_2183.
GeneIDi3719653.
KEGGirsp:RSP_2183.
PATRICi23151370. VBIRhoSph57909_1079.

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
KOiK00130.
OMAiHVYQNIA.
OrthoDBiEOG6BS8QW.
PhylomeDBiQ3J4E9.

Enzyme and pathway databases

UniPathwayiUPA00529; UER00386.
BioCyciRSPH272943:GJAS-790-MONOMER.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00804. BADH.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01804. BADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Entry informationi

Entry nameiBETB_RHOS4
AccessioniPrimary (citable) accession number: Q3J4E9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: May 27, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.