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Q3J3M6 (GLGB_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:RHOS4_10400
ORF Names:RSP_2448
OrganismRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [Reference proteome] [HAMAP]
Taxonomic identifier272943 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Protein attributes

Sequence length728 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7287281,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260686

Sites

Active site4091Nucleophile By similarity
Active site4621Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3J3M6 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: C4B3DDDC265B7161

FASTA72881,458
        10         20         30         40         50         60 
MNVTSKPGEL VPESDVAAIM RGTHGDPFRV LGMHGGGGAP LSVRVFAPQA AEVAVLGQGG 

        70         80         90        100        110        120 
EVLAPLERIG AEGFFAGTVP GEEKFPYRLR FVSGPHEWEA DDPYRFPEVL GELDEYLLGE 

       130        140        150        160        170        180 
GRHYQLYTRL GAHPAEIEGV QGVSFAVWAP NARRVSVVGA FNAWDGRRHP MRKRIGVGVW 

       190        200        210        220        230        240 
ELFVPGLHTG DLYKYELLGP SGERLPLKSD PLSFAQEAPP ATASVVHGLP EAEWHDAGWM 

       250        260        270        280        290        300 
QERESRQRRD APISIYEVHA GSWRQGLDYD ALAEELSAYV REMGFTHVEF LPISEHPFTG 

       310        320        330        340        350        360 
SWGYQPIGLF APTARFGPPE GFARLVDRLH RDGIGVILDW VPAHFPSDAH GLANFDGTHL 

       370        380        390        400        410        420 
YDHADPRQGF HRDWNTQIYN FGRQEVANFL QASALFWLDR YHVDALRVDA VASMLYLDYS 

       430        440        450        460        470        480 
RNAGEWVPNR HGGRENLEAI DFLRGVNERV RLDHPGCITI AEESTAFPQV SRPVEDGGLG 

       490        500        510        520        530        540 
FGFKWNMGWM HDTLGYFRRD PIHRKHHQND LTFGMVYAYS EDFVLPLSHD EVVHGKGSLI 

       550        560        570        580        590        600 
GQMAGDRWQK FANLRAYFGF MWAHPGKKLL FMGGEFAQER EWNHDASLDW HLLDDPSHAG 

       610        620        630        640        650        660 
MKRLVADLNR EYRKRPALHR MDCDPEGFEW IDAGDSENSV LSFLRKAPGE KPVLAVCNLT 

       670        680        690        700        710        720 
PVVRSDYRIG VPEGGEWREI LNSDAAIYGG SDVGNPGGLQ AEEFSWHGRP ASLRLTLPPL 


ATIFVTPA 

« Hide

References

[1]"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000143 Genomic DNA. Translation: ABA78608.1.
RefSeqYP_352509.1. NC_007493.2.

3D structure databases

ProteinModelPortalQ3J3M6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272943.RSP_2448.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

DNASU3720044.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA78608; ABA78608; RSP_2448.
GeneID3720044.
KEGGrsp:RSP_2448.
PATRIC23151949. VBIRhoSph57909_1366.

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAHERCPHA.
OrthoDBEOG6JX7GT.
PhylomeDBQ3J3M6.

Enzyme and pathway databases

BioCycRSPH272943:GJAS-1067-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_RHOS4
AccessionPrimary (citable) accession number: Q3J3M6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 8, 2005
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries