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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei409NucleophileUniRule annotation1
Active sitei462Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:RHOS4_10400
ORF Names:RSP_2448
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
Proteomesi
  • UP000002703 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002606861 – 7281,4-alpha-glucan branching enzyme GlgBAdd BLAST728

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272943.RSP_2448

Structurei

3D structure databases

ProteinModelPortaliQ3J3M6
SMRiQ3J3M6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9C Bacteria
COG0296 LUCA
HOGENOMiHOG000283037
KOiK00700
OMAiEVVHGKS
OrthoDBiPOG091H08D1
PhylomeDBiQ3J3M6

Family and domain databases

Gene3Di2.60.40.101 hit
2.60.40.11801 hit
HAMAPiMF_00685 GlgB, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR037439 Branching_enzy
IPR006407 GlgB
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PANTHERiPTHR43651 PTHR43651, 2 hits
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF02922 CBM_48, 1 hit
PIRSFiPIRSF000463 GlgB, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 2 hits
TIGRFAMsiTIGR01515 branching_enzym, 1 hit

Sequencei

Sequence statusi: Complete.

Q3J3M6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVTSKPGEL VPESDVAAIM RGTHGDPFRV LGMHGGGGAP LSVRVFAPQA
60 70 80 90 100
AEVAVLGQGG EVLAPLERIG AEGFFAGTVP GEEKFPYRLR FVSGPHEWEA
110 120 130 140 150
DDPYRFPEVL GELDEYLLGE GRHYQLYTRL GAHPAEIEGV QGVSFAVWAP
160 170 180 190 200
NARRVSVVGA FNAWDGRRHP MRKRIGVGVW ELFVPGLHTG DLYKYELLGP
210 220 230 240 250
SGERLPLKSD PLSFAQEAPP ATASVVHGLP EAEWHDAGWM QERESRQRRD
260 270 280 290 300
APISIYEVHA GSWRQGLDYD ALAEELSAYV REMGFTHVEF LPISEHPFTG
310 320 330 340 350
SWGYQPIGLF APTARFGPPE GFARLVDRLH RDGIGVILDW VPAHFPSDAH
360 370 380 390 400
GLANFDGTHL YDHADPRQGF HRDWNTQIYN FGRQEVANFL QASALFWLDR
410 420 430 440 450
YHVDALRVDA VASMLYLDYS RNAGEWVPNR HGGRENLEAI DFLRGVNERV
460 470 480 490 500
RLDHPGCITI AEESTAFPQV SRPVEDGGLG FGFKWNMGWM HDTLGYFRRD
510 520 530 540 550
PIHRKHHQND LTFGMVYAYS EDFVLPLSHD EVVHGKGSLI GQMAGDRWQK
560 570 580 590 600
FANLRAYFGF MWAHPGKKLL FMGGEFAQER EWNHDASLDW HLLDDPSHAG
610 620 630 640 650
MKRLVADLNR EYRKRPALHR MDCDPEGFEW IDAGDSENSV LSFLRKAPGE
660 670 680 690 700
KPVLAVCNLT PVVRSDYRIG VPEGGEWREI LNSDAAIYGG SDVGNPGGLQ
710 720
AEEFSWHGRP ASLRLTLPPL ATIFVTPA
Length:728
Mass (Da):81,458
Last modified:November 8, 2005 - v1
Checksum:iC4B3DDDC265B7161
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA Translation: ABA78608.1
RefSeqiWP_011337498.1, NZ_AKVW01000001.1
YP_352509.1, NC_007493.2

Genome annotation databases

EnsemblBacteriaiABA78608; ABA78608; RSP_2448
GeneIDi3720044
KEGGirsp:RSP_2448
PATRICifig|272943.9.peg.1366

Similar proteinsi

Entry informationi

Entry nameiGLGB_RHOS4
AccessioniPrimary (citable) accession number: Q3J3M6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 8, 2005
Last modified: February 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome