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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathway:iglycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei409 – 4091NucleophileUniRule annotation
Active sitei462 – 4621Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-1067-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:RHOS4_10400
ORF Names:RSP_2448
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000002703 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7287281,4-alpha-glucan branching enzyme GlgBPRO_0000260686Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272943.RSP_2448.

Structurei

3D structure databases

ProteinModelPortaliQ3J3M6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiEOG6JX7GT.
PhylomeDBiQ3J3M6.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3J3M6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVTSKPGEL VPESDVAAIM RGTHGDPFRV LGMHGGGGAP LSVRVFAPQA
60 70 80 90 100
AEVAVLGQGG EVLAPLERIG AEGFFAGTVP GEEKFPYRLR FVSGPHEWEA
110 120 130 140 150
DDPYRFPEVL GELDEYLLGE GRHYQLYTRL GAHPAEIEGV QGVSFAVWAP
160 170 180 190 200
NARRVSVVGA FNAWDGRRHP MRKRIGVGVW ELFVPGLHTG DLYKYELLGP
210 220 230 240 250
SGERLPLKSD PLSFAQEAPP ATASVVHGLP EAEWHDAGWM QERESRQRRD
260 270 280 290 300
APISIYEVHA GSWRQGLDYD ALAEELSAYV REMGFTHVEF LPISEHPFTG
310 320 330 340 350
SWGYQPIGLF APTARFGPPE GFARLVDRLH RDGIGVILDW VPAHFPSDAH
360 370 380 390 400
GLANFDGTHL YDHADPRQGF HRDWNTQIYN FGRQEVANFL QASALFWLDR
410 420 430 440 450
YHVDALRVDA VASMLYLDYS RNAGEWVPNR HGGRENLEAI DFLRGVNERV
460 470 480 490 500
RLDHPGCITI AEESTAFPQV SRPVEDGGLG FGFKWNMGWM HDTLGYFRRD
510 520 530 540 550
PIHRKHHQND LTFGMVYAYS EDFVLPLSHD EVVHGKGSLI GQMAGDRWQK
560 570 580 590 600
FANLRAYFGF MWAHPGKKLL FMGGEFAQER EWNHDASLDW HLLDDPSHAG
610 620 630 640 650
MKRLVADLNR EYRKRPALHR MDCDPEGFEW IDAGDSENSV LSFLRKAPGE
660 670 680 690 700
KPVLAVCNLT PVVRSDYRIG VPEGGEWREI LNSDAAIYGG SDVGNPGGLQ
710 720
AEEFSWHGRP ASLRLTLPPL ATIFVTPA
Length:728
Mass (Da):81,458
Last modified:November 8, 2005 - v1
Checksum:iC4B3DDDC265B7161
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA78608.1.
RefSeqiWP_011337498.1. NZ_AKVW01000001.1.
YP_352509.1. NC_007493.2.

Genome annotation databases

EnsemblBacteriaiABA78608; ABA78608; RSP_2448.
GeneIDi3720044.
KEGGirsp:RSP_2448.
PATRICi23151949. VBIRhoSph57909_1366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA78608.1.
RefSeqiWP_011337498.1. NZ_AKVW01000001.1.
YP_352509.1. NC_007493.2.

3D structure databases

ProteinModelPortaliQ3J3M6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272943.RSP_2448.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

DNASUi3720044.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA78608; ABA78608; RSP_2448.
GeneIDi3720044.
KEGGirsp:RSP_2448.
PATRICi23151949. VBIRhoSph57909_1366.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiEOG6JX7GT.
PhylomeDBiQ3J3M6.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciRSPH272943:GJAS-1067-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Entry informationi

Entry nameiGLGB_RHOS4
AccessioniPrimary (citable) accession number: Q3J3M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 8, 2005
Last modified: April 29, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.