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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei93Proton acceptorUniRule annotation1
Binding sitei147SubstrateUniRule annotation1
Binding sitei190SubstrateUniRule annotation1
Binding sitei217Substrate; via amide nitrogenUniRule annotation1
Active sitei267NucleophileUniRule annotation1
Metal bindingi305ZincUniRule annotation1
Metal bindingi307ZincUniRule annotation1
Metal bindingi310ZincUniRule annotation1
Metal bindingi336Zinc; via pros nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:RHOS4_14420
ORF Names:RSP_2804
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
Proteomesi
  • UP000002703 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000168351 – 376Queuine tRNA-ribosyltransferaseAdd BLAST376

Interactioni

Subunit structurei

Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.UniRule annotation

Protein-protein interaction databases

STRINGi272943.RSP_2804.

Structurei

3D structure databases

ProteinModelPortaliQ3J2H4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 97Substrate bindingUniRule annotation5
Regioni248 – 254RNA bindingUniRule annotation7

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
KOiK00773.
OMAiGIDLFDC.
OrthoDBiPOG091H00XO.
PhylomeDBiQ3J2H4.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt. 1 hit.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3J2H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQRFSFELT ATDGRARTGV ISTPRGEIRT PAFMPVGTAG TVKAMLPENV
60 70 80 90 100
RATGADILLG NTYHLMLRPT AERVVRLGGL HRFMNWDRPI LTDSGGFQVM
110 120 130 140 150
SLADLRKLSE EGVTFRSHID GSKHHLSPER SMEIQRLLGS DIVMAFDECP
160 170 180 190 200
ALPATEEAVA QSMRLSMRWA RRSREAFGDR PGHALFGIMQ GGVTRDLREE
210 220 230 240 250
SAAALREIGF EGYAIGGLAV GEGQEAMFGV LDYAPGLLPE DRPRYLMGVG
260 270 280 290 300
KPDDIVGAVE RGVDMMDCVL PSRSGRTGQA WTRRGQVNIK NARHMDDPRP
310 320 330 340 350
LDEACSCPAC RSYSRAYLHH VFRAQEIIAS MLLTWHNLHY YQELMQGLRT
360 370
AIAAGRLGEF VAAFHAARAE GDIEPL
Length:376
Mass (Da):41,508
Last modified:November 8, 2005 - v1
Checksum:i0BD9BDA44D5BDA7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA79010.1.
RefSeqiWP_011337793.1. NZ_AKVW01000001.1.
YP_352911.1. NC_007493.2.

Genome annotation databases

EnsemblBacteriaiABA79010; ABA79010; RSP_2804.
GeneIDi3720589.
KEGGirsp:RSP_2804.
PATRICi23152791. VBIRhoSph57909_1782.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA79010.1.
RefSeqiWP_011337793.1. NZ_AKVW01000001.1.
YP_352911.1. NC_007493.2.

3D structure databases

ProteinModelPortaliQ3J2H4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272943.RSP_2804.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA79010; ABA79010; RSP_2804.
GeneIDi3720589.
KEGGirsp:RSP_2804.
PATRICi23152791. VBIRhoSph57909_1782.

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
KOiK00773.
OMAiGIDLFDC.
OrthoDBiPOG091H00XO.
PhylomeDBiQ3J2H4.

Enzyme and pathway databases

UniPathwayiUPA00392.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt. 1 hit.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTGT_RHOS4
AccessioniPrimary (citable) accession number: Q3J2H4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.