Q3J1A8 (DXS1_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase 1
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase 1
Short name=DXP synthase 1
Short name=DXPS 1

Gene names

Name:	dxs1
Ordered Locus Names:	RHOS4_18580
ORF Names:	RSP_0254

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	1-deoxy-D-xylulose-5-phosphate synthase 1 HAMAP MF_00315		PRO_0000256475

Sequences

	Sequence		Length	Mass (Da)
	Q3J1A8 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: D2AF0116BA3C080D	FASTA	648	68,586
	10 20 30 40 50 60 MTNPTPRPET PLLDRVCCPA DMKALSDAEL ERLADEVRSE VISVVAETGG HLGSSLGVVE 70 80 90 100 110 120 LTVALHAVFN TPTDKLVWDV GHQCYPHKIL TGRREQMRTL RQKGGLSGFT KRSESAYDPF 130 140 150 160 170 180 GAAHSSTSIS AALGFAMGRE LGQPVGDTIA VIGDGSITAG MAYEALNHAG HLNKRLFVIL 190 200 210 220 230 240 NDNDMSIAPP VGALARYLVN LSSKAPFATL RAAADGLEAS LPGPLRDGAR RARQLVTGMP 250 260 270 280 290 300 GGGTLFEELG FTYVGPIDGH DMEALLQTLR AARARTTGPV LIHVVTKKGK GYAPAENAPD 310 320 330 340 350 360 KYHGVNKFDP VTGEQKKSVA NAPNYTKVFG STLTEEAARD PRIVAITAAM PSGTGVDIMQ 370 380 390 400 410 420 KRFPNRVFDV GIAEQHAVTF AAGLAGAGMK PFCAIYSSFL QRGYDQIAHD VALQNLPVRF 430 440 450 460 470 480 VIDRAGLVGA DGATHAGAFD VGFITSLPNM TVMAAADEAE LIHMIATAVA FDEGPIAFRF 490 500 510 520 530 540 PRGEGVGVEM PERGTVLEPG RGRVVREGTD VAILSFGAHL HEALQAAKLL EAEGVSVTVA 550 560 570 580 590 600 DARFSRPLDT GLIDQLVRHH AALVTVEQGA MGGFGAHVMH YLANSGGFDG GLALRVMTLP 610 620 630 640 DRFIEQASPE DMYADAGLRA EDIAATARGA LARGRVMPLR QTAKPRAV

References

[1]

"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000143 Genomic DNA. Translation: ABA79426.1.
RefSeq	YP_353327.1. NC_007493.1.
3D structure databases
ProteinModelPortal	Q3J1A8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3J1A8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3719396.
GenomeReviews	Gene locus RHOS4_18580 in contig CP000143_GR.
KEGG	rsp:RSP_0254.
NMPDR	fig|272943.3.peg.1887.
PATRIC	23153644. VBIRhoSph57909_2197.
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	RFANEHD.
PhylomeDB	Q3J1A8.
ProtClustDB	PRK12571.
Enzyme and pathway databases
BioCyc	RSPH272943:RSP_0254-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR000399. TPP-bd_CS. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS1_RHOS4

Accession

Primary (citable) accession number: Q3J1A8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program