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Q3J1A6 (RCEM_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Reaction center protein M chain
Alternative name(s):
Photosynthetic reaction center M subunit
Gene names
Name:pufM
Ordered Locus Names:RHOS4_18600
ORF Names:RSP_0256
OrganismRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [Reference proteome] [HAMAP]
Taxonomic identifier272943 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter ›

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis By similarity.

Subunit structure

Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H) By similarity.

Subcellular location

Cellular chromatophore membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the reaction center PufL/M/PsbA/D family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 308307Reaction center protein M chain
PRO_0000090418

Regions

Transmembrane54 – 8027Helical; By similarity
Transmembrane111 – 14030Helical; By similarity
Transmembrane143 – 16826Helical; By similarity
Transmembrane198 – 22629Helical; By similarity
Transmembrane260 – 28627Helical; By similarity

Sites

Metal binding1831Magnesium (bacteriochlorophyll b axial ligand) By similarity
Metal binding2031Magnesium (bacteriochlorophyll b axial ligand) By similarity
Metal binding2201Iron By similarity
Metal binding2351Iron By similarity
Metal binding2671Iron By similarity
Binding site2531Quinone A By similarity

Experimental info

Sequence conflict2451A → E in AAF24305. Ref.1

Secondary structure

1 ........................................... 308
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q3J1A6 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 30B3737DF8658250

FASTA30834,509
        10         20         30         40         50         60 
MAEYQNIFSQ VQVRGPADLG MTEDVNLANR SGVGPFSTLL GWFGNAQLGP IYLGSLGVLS 

        70         80         90        100        110        120 
LFSGLMWFFT IGIWFWYQAG WNPAVFLRDL FFFSLEPPAP EYGLSFAAPL KEGGLWLIAS 

       130        140        150        160        170        180 
FFMFVAVWSW WGRTYLRAQA LGMGKHTAWA FLSAIWLWMV LGFIRPILMG SWSEAVPYGI 

       190        200        210        220        230        240 
FSHLDWTNNF SLVHGNLFYN PFHGLSIAFL YGSALLFAMH GATILAVSRF GGERELEQIA 

       250        260        270        280        290        300 
DRGTAAERAA LFWRWTMGFN ATMEGIHRWA IWMAVLVTLT GGIGILLSGT VVDNWYVWGQ 


NHGMAPLN

« Hide

References

« Hide 'large scale' references
[1]"DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1."
Choudhary M., Kaplan S.
Nucleic Acids Res. 28:862-867(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF195122 Genomic DNA. Translation: AAF24305.1.
CP000143 Genomic DNA. Translation: ABA79428.1.
PIRT50761.
RefSeqYP_353329.1. NC_007493.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2WX5X-ray2.63M2-308[»]
ProteinModelPortalQ3J1A6.
SMRQ3J1A6. Positions 2-308.
ModBaseSearch...

Protein-protein interaction databases

STRING272943.RSP_0256.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3719398.
KEGGrsp:RSP_0256.
PATRIC23153648. VBIRhoSph57909_2199.

Phylogenomic databases

eggNOGNOG05026.
HOGENOMHOG000020212.
KOK08929.
OMAGIFPHLD.
ProtClustDBPRK14504.

Enzyme and pathway databases

BioCycRSPH272943:GJAS-1912-MONOMER.

Family and domain databases

Gene3D1.20.85.10. 2 hits.
InterProIPR000484. Photo_RC_L/M.
IPR005781. Photo_RC_M.
[Graphical view]
PfamPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSPR00256. REACTNCENTRE.
SUPFAMSSF81483. Photo_RC_L/M. 1 hit.
TIGRFAMsTIGR01115. pufM. 1 hit.
PROSITEPS00244. REACTION_CENTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRCEM_RHOS4
AccessionPrimary (citable) accession number: Q3J1A6
Secondary accession number(s): P02953, Q9RFB8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 1, 2013
This is version 57 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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