Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Reaction center protein M chain

Gene

pufM

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi183 – 1831Magnesium (bacteriochlorophyll b axial ligand)By similarity
Metal bindingi203 – 2031Magnesium (bacteriochlorophyll b axial ligand)By similarity
Metal bindingi220 – 2201IronBy similarity
Metal bindingi235 – 2351IronBy similarity
Binding sitei253 – 2531Quinone ABy similarity
Metal bindingi267 – 2671IronBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Bacteriochlorophyll, Chlorophyll, Chromophore, Iron, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-1912-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Reaction center protein M chain
Alternative name(s):
Photosynthetic reaction center M subunit
Gene namesi
Name:pufM
Ordered Locus Names:RHOS4_18600
ORF Names:RSP_0256
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
Proteomesi
  • UP000002703 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei54 – 8027HelicalBy similarityAdd
BLAST
Transmembranei111 – 14030HelicalBy similarityAdd
BLAST
Transmembranei143 – 16826HelicalBy similarityAdd
BLAST
Transmembranei198 – 22629HelicalBy similarityAdd
BLAST
Transmembranei260 – 28627HelicalBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Reaction center

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 308307Reaction center protein M chainPRO_0000090418Add
BLAST

Interactioni

Subunit structurei

Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H).By similarity

Protein-protein interaction databases

STRINGi272943.RSP_0256.

Structurei

Secondary structure

1
308
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 83Combined sources
Beta strandi12 – 143Combined sources
Helixi27 – 293Combined sources
Helixi38 – 414Combined sources
Helixi55 – 7824Combined sources
Turni79 – 813Combined sources
Helixi83 – 886Combined sources
Turni89 – 924Combined sources
Helixi100 – 1023Combined sources
Helixi110 – 1123Combined sources
Helixi114 – 14027Combined sources
Helixi146 – 16217Combined sources
Helixi164 – 1696Combined sources
Helixi172 – 1743Combined sources
Helixi180 – 19314Combined sources
Helixi197 – 1993Combined sources
Helixi201 – 22626Combined sources
Helixi228 – 2303Combined sources
Turni231 – 2333Combined sources
Helixi235 – 2406Combined sources
Helixi244 – 25714Combined sources
Helixi265 – 28723Combined sources
Turni289 – 2913Combined sources
Helixi295 – 3006Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WX5X-ray2.63M2-308[»]
4IN5X-ray2.20M1-307[»]
4IN6X-ray2.70M2-303[»]
4N7LX-ray2.85M2-304[»]
ProteinModelPortaliQ3J1A6.
SMRiQ3J1A6. Positions 2-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107R94. Bacteria.
ENOG410ZVGN. LUCA.
HOGENOMiHOG000020212.
KOiK08929.
OMAiIFPHLDW.
OrthoDBiEOG6KDKNT.
PhylomeDBiQ3J1A6.

Family and domain databases

Gene3Di1.20.85.10. 2 hits.
InterProiIPR000484. Photo_RC_L/M.
IPR005781. Photo_RC_M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01115. pufM. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3J1A6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEYQNIFSQ VQVRGPADLG MTEDVNLANR SGVGPFSTLL GWFGNAQLGP
60 70 80 90 100
IYLGSLGVLS LFSGLMWFFT IGIWFWYQAG WNPAVFLRDL FFFSLEPPAP
110 120 130 140 150
EYGLSFAAPL KEGGLWLIAS FFMFVAVWSW WGRTYLRAQA LGMGKHTAWA
160 170 180 190 200
FLSAIWLWMV LGFIRPILMG SWSEAVPYGI FSHLDWTNNF SLVHGNLFYN
210 220 230 240 250
PFHGLSIAFL YGSALLFAMH GATILAVSRF GGERELEQIA DRGTAAERAA
260 270 280 290 300
LFWRWTMGFN ATMEGIHRWA IWMAVLVTLT GGIGILLSGT VVDNWYVWGQ

NHGMAPLN
Length:308
Mass (Da):34,509
Last modified:January 23, 2007 - v3
Checksum:i30B3737DF8658250
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti245 – 2451A → E in AAF24305 (PubMed:10648776).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195122 Genomic DNA. Translation: AAF24305.1.
CP000143 Genomic DNA. Translation: ABA79428.1.
PIRiT50761.
RefSeqiWP_002720420.1. NZ_AKVW01000001.1.
YP_353329.1. NC_007493.2.

Genome annotation databases

EnsemblBacteriaiABA79428; ABA79428; RSP_0256.
GeneIDi3719398.
KEGGirsp:RSP_0256.
PATRICi23153648. VBIRhoSph57909_2199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195122 Genomic DNA. Translation: AAF24305.1.
CP000143 Genomic DNA. Translation: ABA79428.1.
PIRiT50761.
RefSeqiWP_002720420.1. NZ_AKVW01000001.1.
YP_353329.1. NC_007493.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WX5X-ray2.63M2-308[»]
4IN5X-ray2.20M1-307[»]
4IN6X-ray2.70M2-303[»]
4N7LX-ray2.85M2-304[»]
ProteinModelPortaliQ3J1A6.
SMRiQ3J1A6. Positions 2-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272943.RSP_0256.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA79428; ABA79428; RSP_0256.
GeneIDi3719398.
KEGGirsp:RSP_0256.
PATRICi23153648. VBIRhoSph57909_2199.

Phylogenomic databases

eggNOGiENOG4107R94. Bacteria.
ENOG410ZVGN. LUCA.
HOGENOMiHOG000020212.
KOiK08929.
OMAiIFPHLDW.
OrthoDBiEOG6KDKNT.
PhylomeDBiQ3J1A6.

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-1912-MONOMER.

Family and domain databases

Gene3Di1.20.85.10. 2 hits.
InterProiIPR000484. Photo_RC_L/M.
IPR005781. Photo_RC_M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01115. pufM. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1."
    Choudhary M., Kaplan S.
    Nucleic Acids Res. 28:862-867(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Entry informationi

Entry nameiRCEM_RHOS4
AccessioniPrimary (citable) accession number: Q3J1A6
Secondary accession number(s): P02953, Q9RFB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 11, 2015
This is version 74 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.