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Protein

Reaction center protein L chain

Gene

pufL

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi154 – 1541Magnesium (bacteriochlorophyll b axial ligand)By similarity
Metal bindingi174 – 1741Magnesium (bacteriochlorophyll b axial ligand)By similarity
Metal bindingi191 – 1911IronBy similarity
Binding sitei217 – 2171Quinone BBy similarity
Metal bindingi231 – 2311IronBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Bacteriochlorophyll, Chlorophyll, Chromophore, Iron, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-1913-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Reaction center protein L chain
Alternative name(s):
Photosynthetic reaction center L subunit
Gene namesi
Name:pufL
Ordered Locus Names:RHOS4_18610
ORF Names:RSP_0257
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
Proteomesi
  • UP000002703 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 3231CytoplasmicBy similarityAdd
BLAST
Transmembranei33 – 5624HelicalBy similarityAdd
BLAST
Topological domaini57 – 8327PeriplasmicBy similarityAdd
BLAST
Transmembranei84 – 11229HelicalBy similarityAdd
BLAST
Topological domaini113 – 1164CytoplasmicBy similarity
Transmembranei117 – 13923HelicalBy similarityAdd
BLAST
Topological domaini140 – 17132PeriplasmicBy similarityAdd
BLAST
Transmembranei172 – 19928HelicalBy similarityAdd
BLAST
Topological domaini200 – 22526CytoplasmicBy similarityAdd
BLAST
Transmembranei226 – 25126HelicalBy similarityAdd
BLAST
Topological domaini252 – 28231PeriplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Reaction center

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 282281Reaction center protein L chainPRO_0000090408Add
BLAST

Interactioni

Subunit structurei

Reaction center is composed of four bacteriochlorophylls, two bacteriopheophytins, two ubiquinones, one iron, and three highly hydrophobic polypeptide chains (designated L, M, and H).By similarity

Protein-protein interaction databases

STRINGi272943.RSP_0257.

Structurei

Secondary structure

1
282
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni5 – 73Combined sources
Helixi8 – 103Combined sources
Beta strandi17 – 193Combined sources
Turni20 – 234Combined sources
Helixi33 – 5725Combined sources
Turni62 – 643Combined sources
Helixi72 – 743Combined sources
Helixi81 – 833Combined sources
Helixi85 – 11228Combined sources
Helixi117 – 13317Combined sources
Helixi135 – 1406Combined sources
Helixi143 – 1453Combined sources
Helixi153 – 16311Combined sources
Helixi168 – 1703Combined sources
Helixi172 – 19928Combined sources
Helixi210 – 22112Combined sources
Helixi227 – 25024Combined sources
Turni253 – 2553Combined sources
Helixi260 – 2645Combined sources
Helixi265 – 2684Combined sources
Helixi271 – 2744Combined sources
Beta strandi278 – 2803Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WX5X-ray2.63L2-282[»]
4IN5X-ray2.20L1-282[»]
4IN6X-ray2.70L2-282[»]
4N7LX-ray2.85L2-282[»]
ProteinModelPortaliQ3J1A5.
SMRiQ3J1A5. Positions 2-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105VGZ. Bacteria.
ENOG410XVH6. LUCA.
HOGENOMiHOG000230362.
KOiK08928.
OMAiPWLINIA.
OrthoDBiPOG091H150F.
PhylomeDBiQ3J1A5.

Family and domain databases

CDDicd09290. Photo-RC_L. 1 hit.
Gene3Di1.20.85.10. 2 hits.
InterProiIPR005871. Photo_RC_L.
IPR000484. Photo_RC_L/M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01157. pufL. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3J1A5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLSFERKY RVPGGTLVGG NLFDFWVGPF YVGFFGVATF FFAALGIILI
60 70 80 90 100
AWSAVLQGTW NPQLISVYPP ALEYGLGGAP LAKGGLWQII TICATGAFVS
110 120 130 140 150
WALREVEICR KLGIGYHIPF AFAFAILAYL TLVLFRPVMM GAWGYAFPYG
160 170 180 190 200
IWTHLDWVSN TGYTYGNFHY NPAHMIAISF FFTNALALAL HGALVLSAAN
210 220 230 240 250
PEKGKEMRTP DHEDTFFRDL VGYSIGTLGI HRLGLLLSLS AVFFSALCMI
260 270 280
ITGTIWFDQW VDWWQWWVKL PWWANIPGGI NG
Length:282
Mass (Da):31,457
Last modified:January 23, 2007 - v3
Checksum:i044F4D3AF085B136
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551V → E in AAF24304 (PubMed:10648776).Curated
Sequence conflicti59 – 591T → S in AAF24304 (PubMed:10648776).Curated
Sequence conflicti66 – 661S → F in AAF24304 (PubMed:10648776).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195122 Genomic DNA. Translation: AAF24304.1.
CP000143 Genomic DNA. Translation: ABA79429.1.
PIRiT50760.
RefSeqiWP_002720421.1. NZ_AKVW01000001.1.
YP_353330.1. NC_007493.2.

Genome annotation databases

EnsemblBacteriaiABA79429; ABA79429; RSP_0257.
GeneIDi3719399.
KEGGirsp:RSP_0257.
PATRICi23153650. VBIRhoSph57909_2200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195122 Genomic DNA. Translation: AAF24304.1.
CP000143 Genomic DNA. Translation: ABA79429.1.
PIRiT50760.
RefSeqiWP_002720421.1. NZ_AKVW01000001.1.
YP_353330.1. NC_007493.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WX5X-ray2.63L2-282[»]
4IN5X-ray2.20L1-282[»]
4IN6X-ray2.70L2-282[»]
4N7LX-ray2.85L2-282[»]
ProteinModelPortaliQ3J1A5.
SMRiQ3J1A5. Positions 2-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272943.RSP_0257.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA79429; ABA79429; RSP_0257.
GeneIDi3719399.
KEGGirsp:RSP_0257.
PATRICi23153650. VBIRhoSph57909_2200.

Phylogenomic databases

eggNOGiENOG4105VGZ. Bacteria.
ENOG410XVH6. LUCA.
HOGENOMiHOG000230362.
KOiK08928.
OMAiPWLINIA.
OrthoDBiPOG091H150F.
PhylomeDBiQ3J1A5.

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-1913-MONOMER.

Family and domain databases

CDDicd09290. Photo-RC_L. 1 hit.
Gene3Di1.20.85.10. 2 hits.
InterProiIPR005871. Photo_RC_L.
IPR000484. Photo_RC_L/M.
[Graphical view]
PfamiPF00124. Photo_RC. 1 hit.
[Graphical view]
PRINTSiPR00256. REACTNCENTRE.
SUPFAMiSSF81483. SSF81483. 1 hit.
TIGRFAMsiTIGR01157. pufL. 1 hit.
PROSITEiPS00244. REACTION_CENTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRCEL_RHOS4
AccessioniPrimary (citable) accession number: Q3J1A5
Secondary accession number(s): P02954, Q9RFB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 76 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.