Q3J157 (UREF_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease accessory protein UreF

Gene names

Name:	ureF
Ordered Locus Names:	RHOS4_19090
ORF Names:	RSP_0303

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity. HAMAP MF_01385
Subunit structure	UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01385.
Sequence similarities	Belongs to the UreF family.

Ontologies

Keywords
Biological process	Nickel insertion
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Urease accessory protein UreF HAMAP MF_01385		PRO_0000344168

Sequences

	Sequence		Length	Mass (Da)
	Q3J157 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 93682FC2CEB134D4	FASTA	210	21,743
	10 20 30 40 50 60 MSAALLSLVQ WLSPAFPTGA FAYSHGLEWA ISEGEVRDAA SARRWIADVL AFGAGRTDAI 70 80 90 100 110 120 LLAHALRGHD PGALSDLARA LAPAAERLRE TEEQGAAFAA TVAALTGRDL PPRPLPVALG 130 140 150 160 170 180 QAAAPLGLPV AEVLALMLHA FAANLVSAAV RFVPLGQTEG QATLAALHPR IGEIAAESAE 190 200 210 APLDALGSAA LRGDLAAMRH ETQEVRIFKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1." Choudhary M., Kaplan S. Nucleic Acids Res. 28:862-867(2000) [PubMed: 10648776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF195122 Genomic DNA. Translation: AAF24257.1. CP000143 Genomic DNA. Translation: ABA79477.1.
RefSeq	YP_353378.1. NC_007493.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3J157.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3719158.
GenomeReviews	Gene locus RHOS4_19090 in contig CP000143_GR.
KEGG	rsp:RSP_0303.
NMPDR	fig|272943.3.peg.1848.
PATRIC	23153746. VBIRhoSph57909_2248.
Phylogenomic databases
eggNOG	COG0830.
HOGENOM	HBG709295.
OMA	WLSPAFP.
ProtClustDB	CLSK940835.
Enzyme and pathway databases
BioCyc	RSPH272943:RSP_0303-MONOMER.
Family and domain databases
HAMAP	MF_01385. UreF. [Tree]
InterPro	IPR002639. UreF. [Graphical view]
KO	K03188.
Pfam	PF01730. UreF. 1 hit. [Graphical view]
PIRSF	PIRSF009467. Ureas_acces_UreF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UREF_RHOS4

Accession

Primary (citable) accession number: Q3J157
Secondary accession number(s): Q9RFF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program