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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi344 – 3441Zinc; catalyticUniRule annotation
Metal bindingi395 – 3951Zinc; catalyticUniRule annotation
Metal bindingi521 – 5211Zinc; catalyticUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. threonine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. threonyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-2045-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)
Alternative name(s):
Threonyl-tRNA synthetaseUniRule annotation
Short name:
ThrRSUniRule annotation
Gene namesi
Name:thrSUniRule annotation
Ordered Locus Names:RHOS4_19920
ORF Names:RSP_0385
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000002703 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 646646Threonine--tRNA ligasePRO_1000020490Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272943.RSP_0385.

Structurei

3D structure databases

ProteinModelPortaliQ3J0X4.
SMRiQ3J0X4. Positions 246-641.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni247 – 544298CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0441.
HOGENOMiHOG000003880.
KOiK01868.
OMAiKSGHWDK.
OrthoDBiEOG61KBFJ.
PhylomeDBiQ3J0X4.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3J0X4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQISLTFPD GKAREFPAGI TPAEVAASIS TSLGKKAISA SVDGRHYDLQ
60 70 80 90 100
WPIETDAKIA IHTMADEAQA LELIRHDLAH IMARAVQELW PDVKVTIGPV
110 120 130 140 150
VANGWYYDFD REETFTPEDL GAIEKRMKEI INAREAVKTE LWERARAIAY
160 170 180 190 200
YEERGEPFKV ELVQAIPEDQ SIRMYWHGGW QDLCRGPHLQ HTGQVPADAF
210 220 230 240 250
KLMSVAGAYW RGDSANKQLQ RIYGVAFKTR DELKAYLHML EEAAKRDHRK
260 270 280 290 300
LGREMELFHL QEEAPGMVFW HPNGWQIYRT LEDYMRGRLR QAGYKEIRTP
310 320 330 340 350
QVVDRKLWEA SGHWEAYKEN MFIVEVEEEH AKEKRINALK PMNCPCHVQV
360 370 380 390 400
YNQGLKSYRD LPLRLAEFGS CHRYESSGSM HGLMRVRGFV QDDAHIFCTE
410 420 430 440 450
DQIESECAAF IELLSSVYKD LGFDSFEIKL STRPEVRIGS DEAWDKVETA
460 470 480 490 500
LENAIRKVGA AYEIDPGEGA FYGPKLDFKL TDAIGRKWQC GTFQVDPNLP
510 520 530 540 550
TRLGAEYIGE DGAKHRPYML HRAILGSFER FIGILIENYA GKLPFWLAPR
560 570 580 590 600
QVVVASIVSD ADPYVAEVVA ALRARGVRAE ADTRNEKINY KVREHSVGKV
610 620 630 640
PVILAIGMQE VEARSVSVRR LGETRTESMG LDQVVDQLAA DARIPG
Length:646
Mass (Da):73,352
Last modified:November 8, 2005 - v1
Checksum:iBEC094BCB08C81DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA79560.1.
RefSeqiYP_353461.1. NC_007493.2.

Genome annotation databases

EnsemblBacteriaiABA79560; ABA79560; RSP_0385.
GeneIDi3718893.
KEGGirsp:RSP_0385.
PATRICi23153912. VBIRhoSph57909_2331.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA79560.1.
RefSeqiYP_353461.1. NC_007493.2.

3D structure databases

ProteinModelPortaliQ3J0X4.
SMRiQ3J0X4. Positions 246-641.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272943.RSP_0385.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA79560; ABA79560; RSP_0385.
GeneIDi3718893.
KEGGirsp:RSP_0385.
PATRICi23153912. VBIRhoSph57909_2331.

Phylogenomic databases

eggNOGiCOG0441.
HOGENOMiHOG000003880.
KOiK01868.
OMAiKSGHWDK.
OrthoDBiEOG61KBFJ.
PhylomeDBiQ3J0X4.

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-2045-MONOMER.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Entry informationi

Entry nameiSYT_RHOS4
AccessioniPrimary (citable) accession number: Q3J0X4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: March 4, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.