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Q3J0X4 (SYT_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Threonine--tRNA ligase

EC=6.1.1.3
Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS
Gene names
Name:thrS
Ordered Locus Names:RHOS4_19920
ORF Names:RSP_0385
OrganismRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [Reference proteome] [HAMAP]
Taxonomic identifier272943 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Protein attributes

Sequence length646 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP-Rule MF_00184

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00184

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00184

Subcellular location

Cytoplasm HAMAP-Rule MF_00184.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processthreonyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

threonine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 646646Threonine--tRNA ligase HAMAP-Rule MF_00184
PRO_1000020490

Regions

Region247 – 544298Catalytic HAMAP-Rule MF_00184

Sites

Metal binding3441Zinc; catalytic By similarity
Metal binding3951Zinc; catalytic By similarity
Metal binding5211Zinc; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3J0X4 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: BEC094BCB08C81DD

FASTA64673,352
        10         20         30         40         50         60 
MAQISLTFPD GKAREFPAGI TPAEVAASIS TSLGKKAISA SVDGRHYDLQ WPIETDAKIA 

        70         80         90        100        110        120 
IHTMADEAQA LELIRHDLAH IMARAVQELW PDVKVTIGPV VANGWYYDFD REETFTPEDL 

       130        140        150        160        170        180 
GAIEKRMKEI INAREAVKTE LWERARAIAY YEERGEPFKV ELVQAIPEDQ SIRMYWHGGW 

       190        200        210        220        230        240 
QDLCRGPHLQ HTGQVPADAF KLMSVAGAYW RGDSANKQLQ RIYGVAFKTR DELKAYLHML 

       250        260        270        280        290        300 
EEAAKRDHRK LGREMELFHL QEEAPGMVFW HPNGWQIYRT LEDYMRGRLR QAGYKEIRTP 

       310        320        330        340        350        360 
QVVDRKLWEA SGHWEAYKEN MFIVEVEEEH AKEKRINALK PMNCPCHVQV YNQGLKSYRD 

       370        380        390        400        410        420 
LPLRLAEFGS CHRYESSGSM HGLMRVRGFV QDDAHIFCTE DQIESECAAF IELLSSVYKD 

       430        440        450        460        470        480 
LGFDSFEIKL STRPEVRIGS DEAWDKVETA LENAIRKVGA AYEIDPGEGA FYGPKLDFKL 

       490        500        510        520        530        540 
TDAIGRKWQC GTFQVDPNLP TRLGAEYIGE DGAKHRPYML HRAILGSFER FIGILIENYA 

       550        560        570        580        590        600 
GKLPFWLAPR QVVVASIVSD ADPYVAEVVA ALRARGVRAE ADTRNEKINY KVREHSVGKV 

       610        620        630        640 
PVILAIGMQE VEARSVSVRR LGETRTESMG LDQVVDQLAA DARIPG 

« Hide

References

[1]"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000143 Genomic DNA. Translation: ABA79560.1.
RefSeqYP_353461.1. NC_007493.2.

3D structure databases

ProteinModelPortalQ3J0X4.
SMRQ3J0X4. Positions 246-641.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272943.RSP_0385.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA79560; ABA79560; RSP_0385.
GeneID3718893.
KEGGrsp:RSP_0385.
PATRIC23153912. VBIRhoSph57909_2331.

Phylogenomic databases

eggNOGCOG0441.
HOGENOMHOG000003880.
KOK01868.
OMAMNCPSHH.
OrthoDBEOG61KBFJ.
PhylomeDBQ3J0X4.

Enzyme and pathway databases

BioCycRSPH272943:GJAS-2045-MONOMER.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPMF_00184. Thr_tRNA_synth.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR01047. TRNASYNTHTHR.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00418. thrS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYT_RHOS4
AccessionPrimary (citable) accession number: Q3J0X4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: July 9, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries