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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).UniRule annotation

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi344ZincUniRule annotation1
Metal bindingi395Zinc; via tele nitrogenUniRule annotation1
Metal bindingi521Zinc; via pros nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)
Alternative name(s):
Threonyl-tRNA synthetaseUniRule annotation
Short name:
ThrRSUniRule annotation
Gene namesi
Name:thrSUniRule annotation
Ordered Locus Names:RHOS4_19920
ORF Names:RSP_0385
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
Proteomesi
  • UP000002703 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000204901 – 646Threonine--tRNA ligaseAdd BLAST646

Proteomic databases

PRIDEiQ3J0X4

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272943.RSP_0385

Structurei

3D structure databases

ProteinModelPortaliQ3J0X4
SMRiQ3J0X4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni247 – 544CatalyticUniRule annotationAdd BLAST298

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C22 Bacteria
COG0441 LUCA
HOGENOMiHOG000003880
KOiK01868
OMAiFYYDFAY
OrthoDBiPOG091H01SS
PhylomeDBiQ3J0X4

Family and domain databases

CDDicd00771 ThrRS_core, 1 hit
Gene3Di3.10.20.30, 1 hit
3.40.50.800, 1 hit
HAMAPiMF_00184 Thr_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR012675 Beta-grasp_dom_sf
IPR004095 TGS
IPR012676 TGS-like
IPR002320 Thr-tRNA-ligase_IIa
IPR018163 Thr/Ala-tRNA-synth_IIc_edit
IPR033728 ThrRS_core
IPR012947 tRNA_SAD
PANTHERiPTHR11451 PTHR11451, 1 hit
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF02824 TGS, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PF07973 tRNA_SAD, 1 hit
PRINTSiPR01047 TRNASYNTHTHR
SMARTiView protein in SMART
SM00863 tRNA_SAD, 1 hit
SUPFAMiSSF55186 SSF55186, 1 hit
SSF81271 SSF81271, 1 hit
TIGRFAMsiTIGR00418 thrS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Q3J0X4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQISLTFPD GKAREFPAGI TPAEVAASIS TSLGKKAISA SVDGRHYDLQ
60 70 80 90 100
WPIETDAKIA IHTMADEAQA LELIRHDLAH IMARAVQELW PDVKVTIGPV
110 120 130 140 150
VANGWYYDFD REETFTPEDL GAIEKRMKEI INAREAVKTE LWERARAIAY
160 170 180 190 200
YEERGEPFKV ELVQAIPEDQ SIRMYWHGGW QDLCRGPHLQ HTGQVPADAF
210 220 230 240 250
KLMSVAGAYW RGDSANKQLQ RIYGVAFKTR DELKAYLHML EEAAKRDHRK
260 270 280 290 300
LGREMELFHL QEEAPGMVFW HPNGWQIYRT LEDYMRGRLR QAGYKEIRTP
310 320 330 340 350
QVVDRKLWEA SGHWEAYKEN MFIVEVEEEH AKEKRINALK PMNCPCHVQV
360 370 380 390 400
YNQGLKSYRD LPLRLAEFGS CHRYESSGSM HGLMRVRGFV QDDAHIFCTE
410 420 430 440 450
DQIESECAAF IELLSSVYKD LGFDSFEIKL STRPEVRIGS DEAWDKVETA
460 470 480 490 500
LENAIRKVGA AYEIDPGEGA FYGPKLDFKL TDAIGRKWQC GTFQVDPNLP
510 520 530 540 550
TRLGAEYIGE DGAKHRPYML HRAILGSFER FIGILIENYA GKLPFWLAPR
560 570 580 590 600
QVVVASIVSD ADPYVAEVVA ALRARGVRAE ADTRNEKINY KVREHSVGKV
610 620 630 640
PVILAIGMQE VEARSVSVRR LGETRTESMG LDQVVDQLAA DARIPG
Length:646
Mass (Da):73,352
Last modified:November 8, 2005 - v1
Checksum:iBEC094BCB08C81DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA Translation: ABA79560.1
RefSeqiWP_011338194.1, NZ_AKVW01000001.1
YP_353461.1, NC_007493.2

Genome annotation databases

EnsemblBacteriaiABA79560; ABA79560; RSP_0385
GeneIDi3718893
KEGGirsp:RSP_0385
PATRICifig|272943.9.peg.2331

Similar proteinsi

Entry informationi

Entry nameiSYT_RHOS4
AccessioniPrimary (citable) accession number: Q3J0X4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: May 23, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health