Q3J0X4 (SYT_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	RHOS4_19920
ORF Names:	RSP_0385

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [Reference proteome] [HAMAP]

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter › Rhodobacter sphaeroides

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP-Rule MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00184
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00184
Subcellular location	Cytoplasm HAMAP-Rule MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 646	646	Threonine--tRNA ligase HAMAP-Rule MF_00184		PRO_1000020490
Regions
	Region	247 – 544	298	Catalytic HAMAP-Rule MF_00184
Sites
	Metal binding	344	1	Zinc; catalytic By similarity
	Metal binding	395	1	Zinc; catalytic By similarity
	Metal binding	521	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3J0X4 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: BEC094BCB08C81DD	FASTA	646	73,352
	10 20 30 40 50 60 MAQISLTFPD GKAREFPAGI TPAEVAASIS TSLGKKAISA SVDGRHYDLQ WPIETDAKIA 70 80 90 100 110 120 IHTMADEAQA LELIRHDLAH IMARAVQELW PDVKVTIGPV VANGWYYDFD REETFTPEDL 130 140 150 160 170 180 GAIEKRMKEI INAREAVKTE LWERARAIAY YEERGEPFKV ELVQAIPEDQ SIRMYWHGGW 190 200 210 220 230 240 QDLCRGPHLQ HTGQVPADAF KLMSVAGAYW RGDSANKQLQ RIYGVAFKTR DELKAYLHML 250 260 270 280 290 300 EEAAKRDHRK LGREMELFHL QEEAPGMVFW HPNGWQIYRT LEDYMRGRLR QAGYKEIRTP 310 320 330 340 350 360 QVVDRKLWEA SGHWEAYKEN MFIVEVEEEH AKEKRINALK PMNCPCHVQV YNQGLKSYRD 370 380 390 400 410 420 LPLRLAEFGS CHRYESSGSM HGLMRVRGFV QDDAHIFCTE DQIESECAAF IELLSSVYKD 430 440 450 460 470 480 LGFDSFEIKL STRPEVRIGS DEAWDKVETA LENAIRKVGA AYEIDPGEGA FYGPKLDFKL 490 500 510 520 530 540 TDAIGRKWQC GTFQVDPNLP TRLGAEYIGE DGAKHRPYML HRAILGSFER FIGILIENYA 550 560 570 580 590 600 GKLPFWLAPR QVVVASIVSD ADPYVAEVVA ALRARGVRAE ADTRNEKINY KVREHSVGKV 610 620 630 640 PVILAIGMQE VEARSVSVRR LGETRTESMG LDQVVDQLAA DARIPG

References

[1]

"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000143 Genomic DNA. Translation: ABA79560.1.
RefSeq	YP_353461.1. NC_007493.1.
3D structure databases
ProteinModelPortal	Q3J0X4.
SMR	Q3J0X4. Positions 246-641.
ModBase	Search...
Protein-protein interaction databases
STRING	272943.RSP_0385.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3718893.
KEGG	rsp:RSP_0385.
PATRIC	23153912. VBIRhoSph57909_2331.
Phylogenomic databases
eggNOG	COG0441.
HOGENOM	HOG000003880.
KO	K01868.
OMA	KITYKIR.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	RSPH272943:GJAS-2045-MONOMER.
Family and domain databases
Gene3D	3.10.20.30. 1 hit. 3.40.50.800. 1 hit.
HAMAP	MF_00184. Thr_tRNA_synth.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_dom. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-ligase_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_RHOS4

Accession

Primary (citable) accession number: Q3J0X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program