ID   FTHS_CERS4              Reviewed;         557 AA.
AC   Q3J047;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   27-MAR-2024, entry version 93.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=RHOS4_22690; ORFNames=RSP_0663;
OS   Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG
OS   31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (Rhodobacter
OS   sphaeroides).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC   Paracoccaceae; Cereibacter.
OX   NCBI_TaxID=272943;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC
RC   12203 / NCIMB 8253 / ATH 2.4.1.;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C.,
RA   Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.;
RT   "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000143; ABA79837.1; -; Genomic_DNA.
DR   RefSeq; WP_002720843.1; NZ_CP030271.1.
DR   RefSeq; YP_353738.1; NC_007493.2.
DR   AlphaFoldDB; Q3J047; -.
DR   SMR; Q3J047; -.
DR   STRING; 272943.RSP_0663; -.
DR   EnsemblBacteria; ABA79837; ABA79837; RSP_0663.
DR   KEGG; rsp:RSP_0663; -.
DR   PATRIC; fig|272943.9.peg.2613; -.
DR   eggNOG; COG2759; Bacteria.
DR   OrthoDB; 9761733at2; -.
DR   PhylomeDB; Q3J047; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000002703; Chromosome 1.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..557
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000300534"
FT   BINDING         67..74
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   557 AA;  59619 MW;  70FA3F19090AF783 CRC64;
     MAVQTDIEIA RAARKKPIQE IGAGLGIPAE ALIPYGHDKA KVGQGFIRGL EGRPDGKLIL
     VTAINPTPAG EGKTTTTVGL GDGLNRIGKK AVICIREASL GPNFGMKGGA AGGGRAQVVP
     MEDMNLHFTG DFHAITAAHN LLAAMIDNHI YWGNALELDA RRITWRRVMD MNDRALRDTV
     VNLGGVANGF PRQTGFDITV ASEVMAILCL ADDLEDLERR LGRIVVGYRR DKSPVYCRDL
     KAAGAMAVLL KDAMQPNLVQ TIENNPAFVH GGPFANIAHG CNSVIATRTA LKLADYVVTE
     AGFGADLGAE KFFDIKCRLA GLKPSAAVVV ATVRALKMNG GVAREDLGRE DVAALRRGCA
     NLGRHIANVK GFGVPVVVAI NHFTTDTEAE IEAVRAYAAG QGAEAFLCRH WAEGSAGIED
     LAQKVVELAE TPSMFAPLYP DDMPLFEKME TVARRIYHAH DVIADHVIRD QLRTWEEAGY
     GALPVCMAKT QYSFTTDAAI RGAPEGHSIP IREVRLAAGA GFVVAICGEI RTMPGLPSQP
     AAELIHLDEE GRIEGLF
//