Q3J047 (FTHS_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	RHOS4_22690
ORF Names:	RSP_0663

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000300534
Regions
	Nucleotide binding	67 – 74	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3J047 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 70FA3F19090AF783	FASTA	557	59,619
	10 20 30 40 50 60 MAVQTDIEIA RAARKKPIQE IGAGLGIPAE ALIPYGHDKA KVGQGFIRGL EGRPDGKLIL 70 80 90 100 110 120 VTAINPTPAG EGKTTTTVGL GDGLNRIGKK AVICIREASL GPNFGMKGGA AGGGRAQVVP 130 140 150 160 170 180 MEDMNLHFTG DFHAITAAHN LLAAMIDNHI YWGNALELDA RRITWRRVMD MNDRALRDTV 190 200 210 220 230 240 VNLGGVANGF PRQTGFDITV ASEVMAILCL ADDLEDLERR LGRIVVGYRR DKSPVYCRDL 250 260 270 280 290 300 KAAGAMAVLL KDAMQPNLVQ TIENNPAFVH GGPFANIAHG CNSVIATRTA LKLADYVVTE 310 320 330 340 350 360 AGFGADLGAE KFFDIKCRLA GLKPSAAVVV ATVRALKMNG GVAREDLGRE DVAALRRGCA 370 380 390 400 410 420 NLGRHIANVK GFGVPVVVAI NHFTTDTEAE IEAVRAYAAG QGAEAFLCRH WAEGSAGIED 430 440 450 460 470 480 LAQKVVELAE TPSMFAPLYP DDMPLFEKME TVARRIYHAH DVIADHVIRD QLRTWEEAGY 490 500 510 520 530 540 GALPVCMAKT QYSFTTDAAI RGAPEGHSIP IREVRLAAGA GFVVAICGEI RTMPGLPSQP 550 AAELIHLDEE GRIEGLF

References

[1]

"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000143 Genomic DNA. Translation: ABA79837.1.
RefSeq	YP_353738.1. NC_007493.1.
3D structure databases
ProteinModelPortal	Q3J047.
SMR	Q3J047. Positions 6-555.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3J047.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3718273.
GenomeReviews	Gene locus RHOS4_22690 in contig CP000143_GR.
KEGG	rsp:RSP_0663.
NMPDR	fig|272943.3.peg.2964.
PATRIC	23154485. VBIRhoSph57909_2613.
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HBG677721.
OMA	TDFTVTI.
PhylomeDB	Q3J047.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	RSPH272943:RSP_0663-MONOMER.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_RHOS4

Accession

Primary (citable) accession number: Q3J047

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program