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Protein

NAD kinase

Gene

nadK

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei44 – 441Proton acceptorUniRule annotation
Binding sitei144 – 1441NADUniRule annotation
Binding sitei152 – 1521NAD; via carbonyl oxygenUniRule annotation
Binding sitei179 – 1791NAD; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi44 – 452NADUniRule annotation
Nucleotide bindingi114 – 1152NADUniRule annotation
Nucleotide bindingi155 – 1606NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-2495-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:RHOS4_24350, RSP_0825
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000002703 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254NAD kinasePRO_1000005435Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272943.RSP_0825.

Structurei

3D structure databases

ProteinModelPortaliQ3IZN1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000139847.
KOiK00858.
OMAiMNSGKRV.
OrthoDBiEOG6PZXDR.
PhylomeDBiQ3IZN1.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3IZN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPQRIGFVA SNAPVAQEAL NVMAARYGQC PLPEADAIVA LGGDGFMLQT
60 70 80 90 100
LHETQSLDIP VYGMNRGTVG FLMNGYAEDG LRERLAEAEE EILNPLAMTA
110 120 130 140 150
VTGAGEVFHR IAINEVSLLR AGPQAAWLKI SVDGKVRMEE LVCDGALVCT
160 170 180 190 200
PAGSTAYNYS AHGPILPIGA DVLALTAIAP FRPRRWRGAL LPKTALVRFD
210 220 230 240 250
VIDAQKRPVM ADADGRSVRD VVSVEIRTEP AVRHRLLFDP GHGLEERLIR

EQFV
Length:254
Mass (Da):27,522
Last modified:November 8, 2005 - v1
Checksum:i4619AA5A6AE18F1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA80003.1.
RefSeqiWP_011338520.1. NZ_AKVW01000001.1.
YP_353904.1. NC_007493.2.

Genome annotation databases

EnsemblBacteriaiABA80003; ABA80003; RSP_0825.
GeneIDi3718403.
KEGGirsp:RSP_0825.
PATRICi23154833. VBIRhoSph57909_2786.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA80003.1.
RefSeqiWP_011338520.1. NZ_AKVW01000001.1.
YP_353904.1. NC_007493.2.

3D structure databases

ProteinModelPortaliQ3IZN1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272943.RSP_0825.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA80003; ABA80003; RSP_0825.
GeneIDi3718403.
KEGGirsp:RSP_0825.
PATRICi23154833. VBIRhoSph57909_2786.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000139847.
KOiK00858.
OMAiMNSGKRV.
OrthoDBiEOG6PZXDR.
PhylomeDBiQ3IZN1.

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-2495-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Entry informationi

Entry nameiNADK_RHOS4
AccessioniPrimary (citable) accession number: Q3IZN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: May 27, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.