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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei622 – 6221ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-2512-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:RHOS4_24520
ORF Names:RSP_0840
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000002703: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 847847Leucine--tRNA ligasePRO_1000009414Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272943.RSP_0840.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi619 – 6235"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.
PhylomeDBiQ3IZL4.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3IZL4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRYDPAATE SRWQAAWDAA GVFTARHDPA RPKYYVLEMF PYPSGRIHMG
60 70 80 90 100
HVRNYTMGDV VARQKAAAGF SVLHPMGWDA FGMPAENAAM ERGGHPKDWT
110 120 130 140 150
YGNIADMRAQ MKPLGLSIDW SREFATCDPE YYGQQQAMFI DMMEAGLVYR
160 170 180 190 200
KNAVVNWDPV DMTVLANEQV IDGKGWRSGA PVVRRELTQW FFRISDYAGE
210 220 230 240 250
LLEALDTLKD WPEKVRLMQA NWIGQSRGLQ FAFSMAGAPE GFDRLEVYTT
260 270 280 290 300
RPDTLMGASF AAISPDHPLA RHLERHDPEV AEFVAECRRV GTSEEALEKA
310 320 330 340 350
EKKGFDTGLR VRHPFDAAWE LPVYIANFIL MDYGTGAIFG CPAHDQRDFE
360 370 380 390 400
FATKYGLPIR PVFLPEGCEE TALAEAFVPM KSERVHYIRG FAGAEVQTGE
410 420 430 440 450
EGVAAAIAFC ESQGVGRGVT NYRLRDWGIS RQRYWGCPIP VIHCETCGVV
460 470 480 490 500
PEAKENLPVR LPDDVSFDVP GNPLDRHPTW RDCTCPKCGA KARRETDTMD
510 520 530 540 550
TFVDSSWYYA RFTAPRAATP TDAEEADYWM NVDQYIGGIE HAILHLLYSR
560 570 580 590 600
FFARAMQKTG HLPAKAIEPF NALFTQGMVT HEIYLTRDAA GRPVYHLPED
610 620 630 640 650
VTDGKLADGT PVEIIPSAKM SKSKKNVVDP MNIIRQFGAD TARWFVMSDS
660 670 680 690 700
PPERDVEWTA SGAEAASKHL HRVWRLADEI SRADGEANAE DGALDKATAR
710 720 730 740 750
AIAEVTQGVE GFAFNKAIAK LYEFTNTLSR SGAGAEAKKR AMRTMAQLMS
760 770 780 790 800
PMVPHLAEEV WAMLGGEGLV AQAAWPKADP ALLIDDTVTL PIQVNGKRRG
810 820 830 840
EITVPKEMAA SEVEKLVLAD EAVQRALGGA APKKLIVVPG RIVNVVI
Length:847
Mass (Da):94,033
Last modified:November 8, 2005 - v1
Checksum:iE52C27363BB7CAC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA80020.1.
RefSeqiYP_353921.1. NC_007493.2.

Genome annotation databases

EnsemblBacteriaiABA80020; ABA80020; RSP_0840.
GeneIDi3718381.
KEGGirsp:RSP_0840.
PATRICi23154867. VBIRhoSph57909_2803.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000143 Genomic DNA. Translation: ABA80020.1.
RefSeqiYP_353921.1. NC_007493.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272943.RSP_0840.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA80020; ABA80020; RSP_0840.
GeneIDi3718381.
KEGGirsp:RSP_0840.
PATRICi23154867. VBIRhoSph57909_2803.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.
PhylomeDBiQ3IZL4.

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-2512-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Entry informationi

Entry nameiSYL_RHOS4
AccessioniPrimary (citable) accession number: Q3IZL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: February 4, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.