Q3IXX7 (PROA_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	RHOS4_30390
ORF Names:	RSP_3824

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000230020

Sequences

	Sequence		Length	Mass (Da)
	Q3IXX7 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 14ECB68C72712616	FASTA	420	44,730
	10 20 30 40 50 60 MDGTDVTMLM REIGVRARAA AAELAFAEPS RKEEALNAAA EAMLARSDEI LEANGRDLAF 70 80 90 100 110 120 GAEKGLTPAM MDRLKLDAAR IDGIVEGLRA VAGQPDPVGQ VIAEWDRPSG LHIRRVRTPL 130 140 150 160 170 180 GVVGVIYESR PNVTADAGAL CLKSGNAVIL RGGSESFHSS GAIHAALQDG LRQAGLPVDA 190 200 210 220 230 240 IQRVPTRDRA AVAEMLRMVE HIDVIVPRGG KGLVGLVQAE ARVPVFAHLE GICHVYADGE 250 260 270 280 290 300 ADLEKARRVV LNAKTRRTGI CGSAECLLID RAFLAKHGPV LIEDLLKAGV EVRAEGELAQ 310 320 330 340 350 360 VPGTVPAQPE DFGREFLDMI IAAKVVDGVD EAIAHIRRYG SSHTESILTE NDATAERFFR 370 380 390 400 410 420 RLDSAILMRN ASTQFADGGE FGMGAEIGIA TGKMHARGPV GAEQLTSFKY LVTGDGTIRT

References

[1]

"Complete sequence of chromosome 2 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000144 Genomic DNA. Translation: ABA80607.1.
RefSeq	YP_354508.1. NC_007494.1.
3D structure databases
ProteinModelPortal	Q3IXX7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IXX7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3721405.
GenomeReviews	Gene locus RHOS4_30390 in contig CP000144_GR.
KEGG	rsp:RSP_3824.
NMPDR	fig|272943.3.peg.3489.
PATRIC	23156110. VBIRhoSph57909_3410.
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
OMA	YGICGAM.
PhylomeDB	Q3IXX7.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	RSPH272943:RSP_3824-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_RHOS4

Accession

Primary (citable) accession number: Q3IXX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program