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Q3IW88 (TRMB_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:RHOS4_36280
ORF Names:RSP_3593
OrganismRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [Reference proteome] [HAMAP]
Taxonomic identifier272943 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Protein attributes

Sequence length241 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 241241tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000229191

Regions

Region219 – 2224Substrate binding By similarity

Sites

Active site1501 By similarity
Binding site761S-adenosyl-L-methionine By similarity
Binding site1011S-adenosyl-L-methionine By similarity
Binding site1281S-adenosyl-L-methionine By similarity
Binding site1501S-adenosyl-L-methionine By similarity
Binding site1541Substrate By similarity
Binding site1861Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3IW88 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: EC1B6E25470EFBB3

FASTA24126,988
        10         20         30         40         50         60 
MSDDDAPESL RGAADGPAWR NFYGRRSGKT LRPSQKVYLS EDLGRLRPEG ITREENPERR 

        70         80         90        100        110        120 
PLDLGALFGG RPVWLEIGFG GGEHMVHMAA RNPGIGLIGC EPFVNGVAML LGKIRAAGVE 

       130        140        150        160        170        180 
NLLVHPGDVR DLFDVLPEAS VSKAFLNYPD PWPKKRHHRR RFVTQGYLGP LARVLAPGAT 

       190        200        210        220        230        240 
FRVATDIPDY VRQTLEEVPQ AGFDLVSESG EAWEDWLSTR YEQKALREGR VPHYMTFRRQ 


G 

« Hide

References

[1]"Complete sequence of chromosome 2 of Rhodobacter sphaeroides 2.4.1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000144 Genomic DNA. Translation: ABA81196.1.
RefSeqYP_355097.1. NC_007494.2.

3D structure databases

ProteinModelPortalQ3IW88.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272943.RSP_3593.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA81196; ABA81196; RSP_3593.
GeneID3722110.
KEGGrsp:RSP_3593.
PATRIC23157369. VBIRhoSph57909_4029.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMAIGCEPFV.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycRSPH272943:GJAS-3722-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_RHOS4
AccessionPrimary (citable) accession number: Q3IW88
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 8, 2005
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways