Reviewed, UniProtKB/Swiss-Prot Q3IUQ3 (RIFK_NATPD)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	NP0072A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Riboflavin kinase HAMAP MF_01285		PRO_0000322096
Regions
	Nucleotide binding	107 – 112	6	CDP By similarity
	Nucleotide binding	212 – 215	4	CDP By similarity
	Region	1 – 97	97	H-T-H motif-like HAMAP MF_01285
	Region	98 – 232	135	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	136	1	Magnesium By similarity
	Metal binding	138	1	Magnesium By similarity
	Binding site	199	1	FMN By similarity
	Binding site	207	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3IUQ3-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 7B9F4A021E05D190	FASTA	232	25,603
	10 20 30 40 50 60 MSQTTERVGA DELSTLKLVA LDGALDGRVT VTCADLADRL DASNQTASRR LQRLEAAALV 70 80 90 100 110 120 ERDMTGEGQR IAVTEAGERA LQREYADYRR LFEGDADVTL SGVVTSGMGE GRHYISLSGY 130 140 150 160 170 180 MRQFRERLGY EPFPGTLNVD LDDGSVRTRA RMDALDPVKI DGWEDDERTY GPAFCWPAVL 190 200 210 220 230 ETADGDRYGT AHVIAPERTH HGDDQLEIIA PEKLRETLSL DDGDTLSIHV SE

References

[1]	"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed: 16169924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936257 Genomic DNA. Translation: CAI48127.1.
RefSeq	YP_325699.1.
3D structure databases
SMR	Q3IUQ3. Positions 13-218, 98-231.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IUQ3.
Genome annotation databases
GeneID	3700753.
GenomeReviews	Gene locus NP0072A in contig CR936257_GR.
KEGG	nph:NP0072A.
NMPDR	fig|348780.3.peg.693.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10832.
HOGENOM	HBG553349.
OMA	GAYYVRQ.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR002834. Riboflavin_kinase_arc. [Graphical view]
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_NATPD

Accession

Primary (citable) accession number: Q3IUQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	November 8, 2005
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)