Q3IUQ3 (RIFK_NATPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	NP0072A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Riboflavin kinase HAMAP MF_01285		PRO_0000322096
Regions
	Nucleotide binding	107 – 112	6	CDP By similarity
	Nucleotide binding	212 – 215	4	CDP By similarity
	Region	1 – 97	97	H-T-H motif-like HAMAP MF_01285
	Region	98 – 232	135	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	136	1	Magnesium By similarity
	Metal binding	138	1	Magnesium By similarity
	Binding site	199	1	FMN By similarity
	Binding site	207	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3IUQ3 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 7B9F4A021E05D190	FASTA	232	25,603
	10 20 30 40 50 60 MSQTTERVGA DELSTLKLVA LDGALDGRVT VTCADLADRL DASNQTASRR LQRLEAAALV 70 80 90 100 110 120 ERDMTGEGQR IAVTEAGERA LQREYADYRR LFEGDADVTL SGVVTSGMGE GRHYISLSGY 130 140 150 160 170 180 MRQFRERLGY EPFPGTLNVD LDDGSVRTRA RMDALDPVKI DGWEDDERTY GPAFCWPAVL 190 200 210 220 230 ETADGDRYGT AHVIAPERTH HGDDQLEIIA PEKLRETLSL DDGDTLSIHV SE

References

[1]

"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed: 16169924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2160 / ATCC 35678.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936257 Genomic DNA. Translation: CAI48127.1.
RefSeq	YP_325699.1. NC_007426.1.
3D structure databases
ProteinModelPortal	Q3IUQ3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IUQ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3700753.
GenomeReviews	Gene locus NP0072A in contig CR936257_GR.
KEGG	nph:NP0072A.
NMPDR	fig|348780.3.peg.693.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10832.
HOGENOM	HBG553349.
OMA	GRHYISL.
ProtClustDB	CLSK511620.
Enzyme and pathway databases
BioCyc	NPHA348780:NP0072A-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_NATPD

Accession

Primary (citable) accession number: Q3IUQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	November 8, 2005
Last modified:	November 16, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program