Q3IU70 (ENDA_NATPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease

Gene names

Name:	endA
Ordered Locus Names:	NP_0444A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas › Natronomonas pharaonis

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01834
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron. HAMAP-Rule MF_01834
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: HAMAP
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	tRNA-splicing endonuclease HAMAP-Rule MF_01834		PRO_0000309820
Sites
	Active site	271	1	By similarity
	Active site	282	1	By similarity
	Active site	313	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3IU70 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: F5BA9A0C581055A6	FASTA	336	36,543
	10 20 30 40 50 60 MEGHLSDGLV EVGPNGRERY YDSSGYGRPD GAGVRLAPVE AAYLLARGDL DAVDGMGLEA 70 80 90 100 110 120 FLADTPDLVV PFLVYRDLRE RGFYVSPALE GWVADHAGID FVVHPRGDGP WDGTVAYRVR 130 140 150 160 170 180 VVDERGSVRL DSLGDVVLAV VDEESELTYL ETDRPAVDGT SGLEADLTAD GVLLEDRVLC 190 200 210 220 230 240 WDAPPALYEQ GFYGQRMDRE DGPLQLSLLE AAHLAATGVL SVAGGAAAVE ARGRDAEGER 250 260 270 280 290 300 FDRRLTVYRT LRERGVVPKT GFKFGADFRT YADVDSVSEL GHSELLVRVL PADATLSPRD 310 320 330 VALDVRLAHG VRKRMVFALV DDGDEIRWVS VSRLTP

References

[1]

"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2160 / ATCC 35678.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936257 Genomic DNA. Translation: CAI48313.1.
RefSeq	YP_325882.1. NC_007426.1.
3D structure databases
HSSP	HSSP built from PDB template 2CV8 based on UniProtKB Q975R3.
ProteinModelPortal	Q3IU70.
ModBase	Search...
Protein-protein interaction databases
STRING	348780.NP0444A.
Proteomic databases
PRIDE	Q3IU70.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAI48313; CAI48313; NP_0444A.
GeneID	3702672.
KEGG	nph:NP0444A.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1676.
HOGENOM	HOG000226561.
KO	K01170.
OMA	FELKYIV.
ProtClustDB	PRK09300.
Enzyme and pathway databases
BioCyc	NPHA348780:GJX0-231-MONOMER.
Family and domain databases
Gene3D	3.40.1350.10. 2 hits.
HAMAP	MF_01834. EndA_long.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006678. tRNA_intron_Endonuc_N. IPR006676. tRNA_splic. IPR023516. tRNA_splic_arch_long. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 2 hits. PF02778. tRNA_int_endo_N. 2 hits. [Graphical view]
SUPFAM	SSF53032. tRNA_int_endo_C. 2 hits. SSF55267. tRNA_int_endo_N. 2 hits.
TIGRFAMs	TIGR00324. endA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ENDA_NATPD

Accession

Primary (citable) accession number: Q3IU70

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program