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Q3IU70 (ENDA_NATPD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease
Gene names
Name:endA
Ordered Locus Names:NP_0444A
OrganismNatronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis) [Complete proteome] [HAMAP]
Taxonomic identifier348780 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeNatronomonas

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01834

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. HAMAP-Rule MF_01834

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01834

Sequence similarities

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA splicing, via endonucleolytic cleavage and ligation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

tRNA-intron endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336tRNA-splicing endonuclease HAMAP-Rule MF_01834
PRO_0000309820

Sites

Active site2711 By similarity
Active site2821 By similarity
Active site3131 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3IU70 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: F5BA9A0C581055A6

FASTA33636,543
        10         20         30         40         50         60 
MEGHLSDGLV EVGPNGRERY YDSSGYGRPD GAGVRLAPVE AAYLLARGDL DAVDGMGLEA 

        70         80         90        100        110        120 
FLADTPDLVV PFLVYRDLRE RGFYVSPALE GWVADHAGID FVVHPRGDGP WDGTVAYRVR 

       130        140        150        160        170        180 
VVDERGSVRL DSLGDVVLAV VDEESELTYL ETDRPAVDGT SGLEADLTAD GVLLEDRVLC 

       190        200        210        220        230        240 
WDAPPALYEQ GFYGQRMDRE DGPLQLSLLE AAHLAATGVL SVAGGAAAVE ARGRDAEGER 

       250        260        270        280        290        300 
FDRRLTVYRT LRERGVVPKT GFKFGADFRT YADVDSVSEL GHSELLVRVL PADATLSPRD 

       310        320        330 
VALDVRLAHG VRKRMVFALV DDGDEIRWVS VSRLTP 

« Hide

References

[1]"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis."
Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D.
Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35678 / DSM 2160.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR936257 Genomic DNA. Translation: CAI48313.1.
RefSeqYP_325882.1. NC_007426.1.

3D structure databases

ProteinModelPortalQ3IU70.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING348780.NP0444A.

Proteomic databases

PRIDEQ3IU70.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI48313; CAI48313; NP_0444A.
GeneID3702672.
KEGGnph:NP0444A.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1676.
HOGENOMHOG000226561.
KOK01170.
OMARGFYLSP.
ProtClustDBPRK09300.

Enzyme and pathway databases

BioCycNPHA348780:GJX0-231-MONOMER.

Family and domain databases

Gene3D3.40.1350.10. 2 hits.
HAMAPMF_01834. EndA_long.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamPF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view]
SUPFAMSSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsTIGR00324. endA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameENDA_NATPD
AccessionPrimary (citable) accession number: Q3IU70
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 8, 2005
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families