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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei271UniRule annotation1
Active sitei282UniRule annotation1
Active sitei313UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Enzyme and pathway databases

BioCyciNPHA348780:G1GI2-231-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:NP_0444A
OrganismiNatronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis)
Taxonomic identifieri348780 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHaloarculaceaeNatronomonas
Proteomesi
  • UP000002698 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003098201 – 336tRNA-splicing endonucleaseAdd BLAST336

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi348780.NP0444A

Structurei

3D structure databases

ProteinModelPortaliQ3IU70
SMRiQ3IU70
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01701 Archaea
COG1676 LUCA
HOGENOMiHOG000226561
KOiK01170
OMAiGADFRTY
OrthoDBiPOG093Z0DMC

Family and domain databases

Gene3Di3.40.1350.10, 2 hits
HAMAPiMF_01834 EndA_long, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR036740 tRNA_intron_Endonuc_N_sf
IPR006676 tRNA_splic
IPR023516 tRNA_splic_arch_long
PANTHERiPTHR21227 PTHR21227, 3 hits
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PF02778 tRNA_int_endo_N, 2 hits
SUPFAMiSSF53032 SSF53032, 2 hits
SSF55267 SSF55267, 2 hits
TIGRFAMsiTIGR00324 endA, 1 hit

Sequencei

Sequence statusi: Complete.

Q3IU70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGHLSDGLV EVGPNGRERY YDSSGYGRPD GAGVRLAPVE AAYLLARGDL
60 70 80 90 100
DAVDGMGLEA FLADTPDLVV PFLVYRDLRE RGFYVSPALE GWVADHAGID
110 120 130 140 150
FVVHPRGDGP WDGTVAYRVR VVDERGSVRL DSLGDVVLAV VDEESELTYL
160 170 180 190 200
ETDRPAVDGT SGLEADLTAD GVLLEDRVLC WDAPPALYEQ GFYGQRMDRE
210 220 230 240 250
DGPLQLSLLE AAHLAATGVL SVAGGAAAVE ARGRDAEGER FDRRLTVYRT
260 270 280 290 300
LRERGVVPKT GFKFGADFRT YADVDSVSEL GHSELLVRVL PADATLSPRD
310 320 330
VALDVRLAHG VRKRMVFALV DDGDEIRWVS VSRLTP
Length:336
Mass (Da):36,543
Last modified:November 8, 2005 - v1
Checksum:iF5BA9A0C581055A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR936257 Genomic DNA Translation: CAI48313.1
RefSeqiWP_011321949.1, NC_007426.1

Genome annotation databases

EnsemblBacteriaiCAI48313; CAI48313; NP_0444A
GeneIDi3702672
KEGGinph:NP_0444A

Similar proteinsi

Entry informationi

Entry nameiENDA_NATPD
AccessioniPrimary (citable) accession number: Q3IU70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health