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Q3IU70

- ENDA_NATPD

UniProt

Q3IU70 - ENDA_NATPD

Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei271 – 2711UniRule annotation
    Active sitei282 – 2821UniRule annotation
    Active sitei313 – 3131UniRule annotation

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciNPHA348780:GJX0-231-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
    Alternative name(s):
    tRNA-intron endonucleaseUniRule annotation
    Gene namesi
    Name:endAUniRule annotation
    Ordered Locus Names:NP_0444A
    OrganismiNatronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
    Taxonomic identifieri348780 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeNatronomonas
    ProteomesiUP000002698: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336tRNA-splicing endonucleasePRO_0000309820Add
    BLAST

    Proteomic databases

    PRIDEiQ3IU70.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi348780.NP0444A.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3IU70.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000226561.
    KOiK01170.
    OMAiRGFYLSP.

    Family and domain databases

    Gene3Di3.40.1350.10. 2 hits.
    HAMAPiMF_01834. EndA_long.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 2 hits.
    PF02778. tRNA_int_endo_N. 2 hits.
    [Graphical view]
    SUPFAMiSSF53032. SSF53032. 2 hits.
    SSF55267. SSF55267. 2 hits.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q3IU70-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGHLSDGLV EVGPNGRERY YDSSGYGRPD GAGVRLAPVE AAYLLARGDL    50
    DAVDGMGLEA FLADTPDLVV PFLVYRDLRE RGFYVSPALE GWVADHAGID 100
    FVVHPRGDGP WDGTVAYRVR VVDERGSVRL DSLGDVVLAV VDEESELTYL 150
    ETDRPAVDGT SGLEADLTAD GVLLEDRVLC WDAPPALYEQ GFYGQRMDRE 200
    DGPLQLSLLE AAHLAATGVL SVAGGAAAVE ARGRDAEGER FDRRLTVYRT 250
    LRERGVVPKT GFKFGADFRT YADVDSVSEL GHSELLVRVL PADATLSPRD 300
    VALDVRLAHG VRKRMVFALV DDGDEIRWVS VSRLTP 336
    Length:336
    Mass (Da):36,543
    Last modified:November 8, 2005 - v1
    Checksum:iF5BA9A0C581055A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR936257 Genomic DNA. Translation: CAI48313.1.
    RefSeqiYP_325882.1. NC_007426.1.

    Genome annotation databases

    EnsemblBacteriaiCAI48313; CAI48313; NP_0444A.
    GeneIDi3702672.
    KEGGinph:NP0444A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR936257 Genomic DNA. Translation: CAI48313.1 .
    RefSeqi YP_325882.1. NC_007426.1.

    3D structure databases

    ProteinModelPortali Q3IU70.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 348780.NP0444A.

    Proteomic databases

    PRIDEi Q3IU70.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI48313 ; CAI48313 ; NP_0444A .
    GeneIDi 3702672.
    KEGGi nph:NP0444A.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000226561.
    KOi K01170.
    OMAi RGFYLSP.

    Enzyme and pathway databases

    BioCyci NPHA348780:GJX0-231-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1350.10. 2 hits.
    HAMAPi MF_01834. EndA_long.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 2 hits.
    PF02778. tRNA_int_endo_N. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53032. SSF53032. 2 hits.
    SSF55267. SSF55267. 2 hits.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis."
      Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D.
      Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35678 / DSM 2160.

    Entry informationi

    Entry nameiENDA_NATPD
    AccessioniPrimary (citable) accession number: Q3IU70
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3