Reviewed, UniProtKB/Swiss-Prot Q3IU54 (DP2L_NATPD)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Synonyms:	polA2
Ordered Locus Names:	NP0476A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas

Protein attributes

Sequence length	1198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1198	1198	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000294698

Sequences

	Sequence		Length	Mass (Da)
	Q3IU54-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 0F886B4A2B484FFA	FASTA	1,198	132,156
	10 20 30 40 50 60 MREDDEQYFR RLESDLDTAL SVAREARKRG GDPTEDIEIP IAKDMADRVE NILGIDGVAE 70 80 90 100 110 120 RVREMEADPD LSREEAALEL AADFANDEVG DYETRAGKVE GAVRTAVALL TEGVVAAPIE 130 140 150 160 170 180 GIDRVELLEN DDGTEFINIY YAGPIRSAGG TAQALSVLVA DYTRTLIGID EYKARNDETE 190 200 210 220 230 240 RYAEELSLYD DETGLQYAPK DEETKFIAEH MPIMLDGEAT GDEEVSGFRD LERVDTNSAR 250 260 270 280 290 300 GGMCLVLGEG IAQKAPKIQR YTSQLDEVDW PWLQDLIDGT YKTGEDTDEA DADSDDGTDE 310 320 330 340 350 360 DAADDSDIDD SSAGDEEADA PSGPPRPDPQ KKFLRDLIAG RPVFGHPSEP GGFRLRYGRA 370 380 390 400 410 420 RNHGFATAGV HPAAMHLIDD FLATGTQIKT ERPGKAAGVV PVDTIEGPTV KLANGDVRRV 430 440 450 460 470 480 DDPEEALEIR NGVEAILDLG EYLVNYGEFV ENNHPLSPAA YAPEWWTQDL AAAGADVQAL 490 500 510 520 530 540 ADSPRVDLEH PSAEQALEWA ERYDAPLHPE YTYLWHDLDV ERFEALAAAA SEGHWAETDG 550 560 570 580 590 600 AQQTRPPDGA AESTLVVPRS EPVREALEVL LVEHTQGEET LTVPDAEPLV RSLGLTEGLE 610 620 630 640 650 660 RTWDELSTAA RSYDDGDNAV RAVNEVAPFE VRERAPTRIG CRMGRPEKSE KRDLSPAVHT 670 680 690 700 710 720 LFPIGEAGGS QRDIAEAATH AESMQDTPGE VEVQVGRRQC PACDTETFRA RCPDCESVTD 730 740 750 760 770 780 PRYVCYDCNI DVDPDESGRA ICPNCEREAD AVQNRTVNVH EEYRSALSNV GERENAFDVL 790 800 810 820 830 840 KGVKGLSSEH KVPEPIEKGV LRAKHGVSAF KDGTVRYDMT DLPVTSVRAA ELDVTADQLR 850 860 870 880 890 900 SLGYETDIHG EPLTHEDQLV ELRVQDVVLS NGAAEHMLRT ADFVDDLLEQ YYGLEPFYDA 910 920 930 940 950 960 EDRDDIVGEL VFGMAPHTSA AVVGRVIGFT SAAVGYAHPY FHAAKRRNCD GDEDCVMLLL 970 980 990 1000 1010 1020 DGLLNFSKTF LPDQRGGRMD APLVMSSRID PAEIDDEAHN MDIVSSYPRA FFEATREMAD 1030 1040 1050 1060 1070 1080 PGEVESLIQL GEDTLGTADE YRGFEHTHDT VDLAAGPDLS AYKTLGDMME KMDAQLELSR 1090 1100 1110 1120 1130 1140 KLRSVDETDV AERVIEYHFL PDIIGNLRAF SRQETRCLDC GEKYRRVPLT GDCRECGGRV 1150 1160 1170 1180 1190 NLTVHQGSVN KYIDTAIQVA EEYGCREYTK QRLRILDRAV ESIFEDDTNK QSGIADFM

References

[1]	"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed: 16169924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR936257 Genomic DNA. Translation: CAI48329.1.
RefSeq	YP_325898.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3703041.
GenomeReviews	Gene locus NP0476A in contig CR936257_GR.
KEGG	nph:NP0476A.
NMPDR	fig|348780.3.peg.292.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3IU54.
OMA	Q3IU54. NCDGDED.
Enzyme and pathway databases
BioCyc	NPHA348780:NP0476A-MON.
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_NATPD

Accession

Primary (citable) accession number: Q3IU54

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	November 8, 2005
Last modified:	May 5, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)