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Q3IU43 (MDH_NATPD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:NP_0498A
OrganismNatronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis) [Complete proteome] [HAMAP]
Taxonomic identifier348780 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeNatronomonas

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 304304Malate dehydrogenase HAMAP-Rule MF_00487
PRO_0000241972

Regions

Nucleotide binding8 – 147NAD By similarity
Nucleotide binding119 – 1213NAD By similarity

Sites

Active site1761Proton acceptor By similarity
Binding site341NAD By similarity
Binding site831Substrate By similarity
Binding site891Substrate By similarity
Binding site961NAD By similarity
Binding site1211Substrate By similarity
Binding site1521Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3IU43 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 7F744512FA379A42

FASTA30432,719
        10         20         30         40         50         60 
MTKVSIIGAA GTVGAAAGYN IALRDIADEL VFVDIPDQRE TTIGQAADAN HGIAYDSNTT 

        70         80         90        100        110        120 
VVQGEYEDTA GSDVVVITAG IPRKEGQTRI DLAGDNAPIM EDIGASLDEY NDDYVSITTS 

       130        140        150        160        170        180 
NPVDLLNRHL YEAGDRDRHK VIGFGGRLDS ARFRYVLSER FDVPVQNVEA TILGEHGDAQ 

       190        200        210        220        230        240 
VPVFSKVRVD GADPEFDGDE KEEILGDLQE SAMDVISRKG ATQWGPATGV AHMVEAVLND 

       250        260        270        280        290        300 
TGEVLPGSLV LDGEYGYEDT AFGVPVKLGA NGIEEVVEWD LDDYESELMD DAAEKLSDQY 


DKIS 

« Hide

References

[1]"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis."
Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D.
Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35678 / DSM 2160.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR936257 Genomic DNA. Translation: CAI48340.1.
RefSeqYP_325909.1. NC_007426.1.

3D structure databases

ProteinModelPortalQ3IU43.
SMRQ3IU43. Positions 2-304.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING348780.NP0498A.

Proteomic databases

PRIDEQ3IU43.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI48340; CAI48340; NP_0498A.
GeneID3702903.
KEGGnph:NP0498A.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000213793.
KOK00024.
OMASTMVKDM.
ProtClustDBPRK06223.

Enzyme and pathway databases

BioCycNPHA348780:GJX0-258-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. SSF56327. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_NATPD
AccessionPrimary (citable) accession number: Q3IU43
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 8, 2005
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families