Q3IU43 (MDH_NATPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	NP_0498A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas › Natronomonas pharaonis

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_0000241972
Regions
	Nucleotide binding	8 – 14	7	NAD By similarity
	Nucleotide binding	119 – 121	3	NAD By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	96	1	NAD By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3IU43 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 7F744512FA379A42	FASTA	304	32,719
	10 20 30 40 50 60 MTKVSIIGAA GTVGAAAGYN IALRDIADEL VFVDIPDQRE TTIGQAADAN HGIAYDSNTT 70 80 90 100 110 120 VVQGEYEDTA GSDVVVITAG IPRKEGQTRI DLAGDNAPIM EDIGASLDEY NDDYVSITTS 130 140 150 160 170 180 NPVDLLNRHL YEAGDRDRHK VIGFGGRLDS ARFRYVLSER FDVPVQNVEA TILGEHGDAQ 190 200 210 220 230 240 VPVFSKVRVD GADPEFDGDE KEEILGDLQE SAMDVISRKG ATQWGPATGV AHMVEAVLND 250 260 270 280 290 300 TGEVLPGSLV LDGEYGYEDT AFGVPVKLGA NGIEEVVEWD LDDYESELMD DAAEKLSDQY DKIS

References

[1]

"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2160 / ATCC 35678.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936257 Genomic DNA. Translation: CAI48340.1.
RefSeq	YP_325909.1. NC_007426.1.
3D structure databases
ProteinModelPortal	Q3IU43.
SMR	Q3IU43. Positions 2-304.
ModBase	Search...
Protein-protein interaction databases
STRING	348780.NP0498A.
Proteomic databases
PRIDE	Q3IU43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAI48340; CAI48340; NP_0498A.
GeneID	3702903.
KEGG	nph:NP0498A.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213793.
KO	K00024.
OMA	AIACDQK.
ProtClustDB	PRK06223.
Enzyme and pathway databases
BioCyc	NPHA348780:GJX0-258-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_NATPD

Accession

Primary (citable) accession number: Q3IU43

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program