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Q3IU11

- LIPA_NATPD

UniProt

Q3IU11 - LIPA_NATPD

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Protein
Probable lipoyl synthase
Gene
lipA, NP_0562A
Organism
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi37 – 371Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi42 – 421Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi48 – 481Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi67 – 671Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi71 – 711Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi74 – 741Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciNPHA348780:GJX0-290-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:NP_0562A
OrganismiNatronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
Taxonomic identifieri348780 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeNatronomonas
ProteomesiUP000002698: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Probable lipoyl synthaseUniRule annotation
PRO_0000325322Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi348780.NP0562A.

Structurei

3D structure databases

ProteinModelPortaliQ3IU11.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHPHIPTK.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3IU11-1 [UniParc]FASTAAdd to Basket

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MSSRRRKPEW LRTRPPSGRR FTEIKETLRD RDLNTVCEEA NCPNLGDCWS    50
GRDGPGTATF MLLGDRCSRG CNFCDVATGG MDPLDPDEPA NVAEAVAEIG 100
LDYVVLTSVD RDDLDDGGAG HFAETIREIK RRDTSVLVEA LIPDFQGDTD 150
AVDRIIDADP DVVAHNVETV ERLQWPVRDR RAGYEQSLDV LRQIAAADGC 200
YAKTSLMLGV GEYAHEVYRT LGDLSEVGVD IVTFGQYLQP SRSHLEVFEY 250
VTPDTFDVWK RVAETEFGFL YCASGPMVRS SFKAGELFVE ALERDGLDIE 300
AARAAAERQ 309
Length:309
Mass (Da):34,323
Last modified:November 8, 2005 - v1
Checksum:i1B7FC3BE5C9574A6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR936257 Genomic DNA. Translation: CAI48372.1.
RefSeqiWP_011322008.1. NC_007426.1.
YP_325941.1. NC_007426.1.

Genome annotation databases

EnsemblBacteriaiCAI48372; CAI48372; NP_0562A.
GeneIDi3702887.
KEGGinph:NP0562A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR936257 Genomic DNA. Translation: CAI48372.1 .
RefSeqi WP_011322008.1. NC_007426.1.
YP_325941.1. NC_007426.1.

3D structure databases

ProteinModelPortali Q3IU11.
ModBasei Search...

Protein-protein interaction databases

STRINGi 348780.NP0562A.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI48372 ; CAI48372 ; NP_0562A .
GeneIDi 3702887.
KEGGi nph:NP0562A.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HPHIPTK.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci NPHA348780:GJX0-290-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis."
    Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D.
    Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35678 / DSM 2160.

Entry informationi

Entry nameiLIPA_NATPD
AccessioniPrimary (citable) accession number: Q3IU11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 8, 2005
Last modified: September 3, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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