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Q3ITY7

- SYI_NATPD

UniProt

Q3ITY7 - SYI_NATPD

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei607 – 6071ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciNPHA348780:GJX0-315-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:NP_0610A
    OrganismiNatronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
    Taxonomic identifieri348780 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeNatronomonas
    ProteomesiUP000002698: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10611061Isoleucine--tRNA ligasePRO_0000098585Add
    BLAST

    Proteomic databases

    PRIDEiQ3ITY7.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi348780.NP0610A.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3ITY7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi50 – 6011"HIGH" regionAdd
    BLAST
    Motifi604 – 6085"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiKPVHWCL.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q3ITY7-1 [UniParc]FASTAAdd to Basket

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    MSRFAEVDDQ YDPDAVEDRV FDYWDEVDAY ERTVEHRADG EDFFFVDGPP     50
    YTSGSAHMGT TWNKTLKDAY IRYHRMRGYN VTDRPGYDMH GLPIETKVEE 100
    RLGFDNKKDI EEFGEQNFID ECKSFAEEQL EGLQEDFKSF GVWMDWDDPY 150
    KTVDPEYMEA AWWGFSKAHD RDLVEQGQRS ISQCPRCETA IANNEVEYDH 200
    VEDPSIYVKF PLAEREGHLV IWTTTPWTVP ANTFVAVDEE MTYQAIEVDT 250
    GDGTETLYVG EPCVEDVVDH GGYEDYEVVG EHEGSDLVGW RYEHPLREEV 300
    PEAPAFEGAL EVYGADYVEA DRTGLVHSAP GHGEVDFERG QELGLSVFCP 350
    VGPDGVYEDA AGDYAGQFVK DADAAIMDDL EAKGLLLSRG TVNHDYGHCW 400
    RCDTPIIQMV TDQWFITVTD IKDELLANME ESEWFPQEAR DNRFRSFIEE 450
    SPDWNVSRQR YWGIPIPIWT PDDWSGDVEE AIVVSTREEL AERVDQDIDP 500
    ESVDLHKDTV DDLTITADGT TYTRVPDVFD VWLDSSVASW GTLGYPGNED 550
    DFEELWPADL IMEAHDQTRG WFWSQLGMGS AALGEAPYET VLMHGWALAE 600
    DGRKMSKSIG NIVAPQEAID RHGADPMRLF LLTQNPQGDD MRFSWDEMEN 650
    RQRDLNILWN VFRFPLPYMR LDDFDPDAVA LDEAALETVD EWVLSRLSTV 700
    TAEMTDHWEN FRQDKALDEL LAFIVEDVSR FYIQVVRERM WEEETSESKL 750
    AAYATLHHVL VETTKLLAPY APFVAEEIYG TLTGDGGHET VHMADWPDPD 800
    ERFRDPQLET DVDIVAAVEE AGSNARQQAE RKLRWPVTRV VVAADDDRAA 850
    EAVDRHRDLL RDRLNAREIE LVEPGSDWEE LSYTARADMS VLGPTFGDEA 900
    GEVMNAINAV SVTDTAVEAL EAAVETELGR DIELTDEMVS FNTETPEGVE 950
    GTAFTVDGDD RGVVYVDTAL TEDIESEGYA REVIRRVQEM RKDLDLDIEA 1000
    EIRVDLDIAD ERVATLVDEH EGLIADEVRA AEFADLNAGH ERVWDVESTD 1050
    ITITIDPVSE R 1061
    Length:1,061
    Mass (Da):120,589
    Last modified:November 8, 2005 - v1
    Checksum:i2B24147A2FCB2EA2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR936257 Genomic DNA. Translation: CAI48396.1.
    RefSeqiWP_011322032.1. NC_007426.1.
    YP_325965.1. NC_007426.1.

    Genome annotation databases

    EnsemblBacteriaiCAI48396; CAI48396; NP_0610A.
    GeneIDi3702863.
    KEGGinph:NP0610A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR936257 Genomic DNA. Translation: CAI48396.1 .
    RefSeqi WP_011322032.1. NC_007426.1.
    YP_325965.1. NC_007426.1.

    3D structure databases

    ProteinModelPortali Q3ITY7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 348780.NP0610A.

    Proteomic databases

    PRIDEi Q3ITY7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI48396 ; CAI48396 ; NP_0610A .
    GeneIDi 3702863.
    KEGGi nph:NP0610A.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi KPVHWCL.

    Enzyme and pathway databases

    BioCyci NPHA348780:GJX0-315-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis."
      Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D.
      Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35678 / DSM 2160.

    Entry informationi

    Entry nameiSYI_NATPD
    AccessioniPrimary (citable) accession number: Q3ITY7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3