SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q3ITY7

- SYI_NATPD

UniProt

Q3ITY7 - SYI_NATPD

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, NP_0610A
Organism
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei607 – 6071ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNPHA348780:GJX0-315-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:NP_0610A
OrganismiNatronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
Taxonomic identifieri348780 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeNatronomonas
ProteomesiUP000002698: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10611061Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098585Add
BLAST

Proteomic databases

PRIDEiQ3ITY7.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi348780.NP0610A.

Structurei

3D structure databases

ProteinModelPortaliQ3ITY7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionUniRule annotation
Add
BLAST
Motifi604 – 6085"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3ITY7-1 [UniParc]FASTAAdd to Basket

« Hide

MSRFAEVDDQ YDPDAVEDRV FDYWDEVDAY ERTVEHRADG EDFFFVDGPP     50
YTSGSAHMGT TWNKTLKDAY IRYHRMRGYN VTDRPGYDMH GLPIETKVEE 100
RLGFDNKKDI EEFGEQNFID ECKSFAEEQL EGLQEDFKSF GVWMDWDDPY 150
KTVDPEYMEA AWWGFSKAHD RDLVEQGQRS ISQCPRCETA IANNEVEYDH 200
VEDPSIYVKF PLAEREGHLV IWTTTPWTVP ANTFVAVDEE MTYQAIEVDT 250
GDGTETLYVG EPCVEDVVDH GGYEDYEVVG EHEGSDLVGW RYEHPLREEV 300
PEAPAFEGAL EVYGADYVEA DRTGLVHSAP GHGEVDFERG QELGLSVFCP 350
VGPDGVYEDA AGDYAGQFVK DADAAIMDDL EAKGLLLSRG TVNHDYGHCW 400
RCDTPIIQMV TDQWFITVTD IKDELLANME ESEWFPQEAR DNRFRSFIEE 450
SPDWNVSRQR YWGIPIPIWT PDDWSGDVEE AIVVSTREEL AERVDQDIDP 500
ESVDLHKDTV DDLTITADGT TYTRVPDVFD VWLDSSVASW GTLGYPGNED 550
DFEELWPADL IMEAHDQTRG WFWSQLGMGS AALGEAPYET VLMHGWALAE 600
DGRKMSKSIG NIVAPQEAID RHGADPMRLF LLTQNPQGDD MRFSWDEMEN 650
RQRDLNILWN VFRFPLPYMR LDDFDPDAVA LDEAALETVD EWVLSRLSTV 700
TAEMTDHWEN FRQDKALDEL LAFIVEDVSR FYIQVVRERM WEEETSESKL 750
AAYATLHHVL VETTKLLAPY APFVAEEIYG TLTGDGGHET VHMADWPDPD 800
ERFRDPQLET DVDIVAAVEE AGSNARQQAE RKLRWPVTRV VVAADDDRAA 850
EAVDRHRDLL RDRLNAREIE LVEPGSDWEE LSYTARADMS VLGPTFGDEA 900
GEVMNAINAV SVTDTAVEAL EAAVETELGR DIELTDEMVS FNTETPEGVE 950
GTAFTVDGDD RGVVYVDTAL TEDIESEGYA REVIRRVQEM RKDLDLDIEA 1000
EIRVDLDIAD ERVATLVDEH EGLIADEVRA AEFADLNAGH ERVWDVESTD 1050
ITITIDPVSE R 1061
Length:1,061
Mass (Da):120,589
Last modified:November 8, 2005 - v1
Checksum:i2B24147A2FCB2EA2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR936257 Genomic DNA. Translation: CAI48396.1.
RefSeqiWP_011322032.1. NC_007426.1.
YP_325965.1. NC_007426.1.

Genome annotation databases

EnsemblBacteriaiCAI48396; CAI48396; NP_0610A.
GeneIDi3702863.
KEGGinph:NP0610A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR936257 Genomic DNA. Translation: CAI48396.1 .
RefSeqi WP_011322032.1. NC_007426.1.
YP_325965.1. NC_007426.1.

3D structure databases

ProteinModelPortali Q3ITY7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 348780.NP0610A.

Proteomic databases

PRIDEi Q3ITY7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI48396 ; CAI48396 ; NP_0610A .
GeneIDi 3702863.
KEGGi nph:NP0610A.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KPVHWCL.

Enzyme and pathway databases

BioCyci NPHA348780:GJX0-315-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis."
    Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D.
    Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35678 / DSM 2160.

Entry informationi

Entry nameiSYI_NATPD
AccessioniPrimary (citable) accession number: Q3ITY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 8, 2005
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi