Q3ITT7 (SYA_NATPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	NP0710A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas

Protein attributes

Sequence length	932 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 932	932	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000075269
Sites
	Metal binding	623	1	Zinc By similarity
	Metal binding	627	1	Zinc By similarity
	Metal binding	726	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ITT7 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 73D472E450675543	FASTA	932	103,353
	10 20 30 40 50 60 MSDLDEEYQL DYFHEEGFER KECPSCGAHF WTRDSERDIC GEPPCADYDF IGDPGFDTEY 70 80 90 100 110 120 SLEEMREAFL SFFEDHDHER IEPYPVAANR WRDDVLLTQA SIYDFQPLVT SGETPPPANP 130 140 150 160 170 180 LTISQPCIRM QDIDNVGKTG RHTMAFEMMA HHAFNAREDI DDPDQYAYEG EVYWKSETVA 190 200 210 220 230 240 YCDQLLDELG ADIEDVTYIE DPWVGGGNAG PAIEVIYRGL ELATLVFMCM EQDPDGDYEL 250 260 270 280 290 300 KDGNRYSYMD TYVVDTGYGL ERWTWMSQGT PTVYEAVYPE MISFLKDNAG LDYSDREESL 310 320 330 340 350 360 VNRAARLSGK LDIDDVDDVE AARDDIADEL GVETDELRAL VEPLEDIYAI ADHCRTLAYM 370 380 390 400 410 420 LGDGIVPSNV GTGYLARMVL RRTKRLADGV GVDAPLDELV DMQAERLDYE NRDTVRDIVR 430 440 450 460 470 480 TEVEKYRETL ERGRRHVERL AEEYAQKGDP IPLDEVIELY DSRGIQPETV EEIAADHGAD 490 500 510 520 530 540 VEIPDDFYSL VAERHGEADA DADDGTLAGD DDRIADLPET EKLYYEEPER TDFEAVVLDV 550 560 570 580 590 600 IERDADGETV YDVALDQTMF YPEGGGQPAD TGTLSTDDVA AEVTDVQETN GVVLHRTDEA 610 620 630 640 650 660 PGKGEFVRGQ IDGVRRRRLM QHHTATHIVG HAARQVLGDH VRQAGAQKGV ESARFDIRHY 670 680 690 700 710 720 ERISREEVKR IERVANNIVT DNLPVKQEWP KRNEAEADYG FDIYQGGIPP GETLRLIQVG 730 740 750 760 770 780 EDVQACAGTH VLQTGDIGTI KILSTERVQD GVERLVFAAG DAAIEATQRT EDALYDTAEV 790 800 810 820 830 840 LDVSPQEVPA TAERFFEEWK DRGKRIEELK EQLAEARAHG GDGGEEVDLG GTTAVVQRVD 850 860 870 880 890 900 GDMDDLRATA NALVEDGTVA VLGSGDDSAT FVVAVPDNVD INAGAVVGEL ADRVGGGGGG 910 920 930 PPDFAQGGGP DVDSLDEALD AAPEILRSML EA

References

[1]

"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed: 16169924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2160 / ATCC 35678.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936257 Genomic DNA. Translation: CAI48446.1.
RefSeq	YP_326015.1. NC_007426.1.
3D structure databases
ProteinModelPortal	Q3ITT7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ITT7.
Proteomic databases
PRIDE	Q3ITT7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3702499.
GenomeReviews	Gene locus NP0710A in contig CR936257_GR.
KEGG	nph:NP0710A.
NMPDR	fig|348780.3.peg.409.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04130.
HOGENOM	HBG392147.
OMA	MFTNSGM.
PhylomeDB	Q3ITT7.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	NPHA348780:NP0710A-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_NATPD

Accession

Primary (citable) accession number: Q3ITT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program