Q3IS99 (SYP_NATPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	NP1796A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Proline--tRNA ligase HAMAP MF_01571		PRO_0000249166

Sequences

	Sequence		Length	Mass (Da)
	Q3IS99 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 615F741B85F9EF52	FASTA	482	54,269
	10 20 30 40 50 60 MTDQELGITE SKEHNPGEWY AEVVQKAGLA DYAPMGGFIV TRPRGYALWE RLQDHLDGWF 70 80 90 100 110 120 KETGVQNTYF PMFIPESYLE REKDIVDGFD PEVAWVTHGG HDELEERLAV RPTSESIITP 130 140 150 160 170 180 FIAEWVRSYR DLPLRVNQWC SVVRWEATET KPFFRTKEFL WQEGHTAHAT EESAWDETMT 190 200 210 220 230 240 RLEQYERLYE EVMAIPGMTG RKPEHDKFPG ADTTTTIEAL MPDGKSVQAG TSHYLGTSFA 250 260 270 280 290 300 EAFDIEYTDE DETKQTAHTT SWGLSWRALG ALIMTHSDDQ GLVIPPALAP TQVVVVPIWQ 310 320 330 340 350 360 ADTEEAVKEY AADLAAELDE QFRVELDDRD ERNPGFKFNE HELQGVPLRI EIGPNEVEDE 370 380 390 400 410 420 AATLVHRPDG ESDVAERESI TDAVDEALET VYAKLYASAE ATLEENIRKA HGRGEILGTL 430 440 450 460 470 480 GQHGGYVKTG WCGDEACEAE IKDEIAAEIV MLPLDEDEPP VYDTCGVCGD EATETAYFAK SY

References

[1]

"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed: 16169924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2160 / ATCC 35678.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936257 Genomic DNA. Translation: CAI48989.1.
RefSeq	YP_326554.1. NC_007426.1.
3D structure databases
ProteinModelPortal	Q3IS99.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IS99.
Proteomic databases
PRIDE	Q3IS99.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3703057.
GenomeReviews	Gene locus NP1796A in contig CR936257_GR.
KEGG	nph:NP1796A.
NMPDR	fig|348780.3.peg.1093.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04466.
HOGENOM	HBG334108.
OMA	KFAEYEL.
PhylomeDB	Q3IS99.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	NPHA348780:NP1796A-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_NATPD

Accession

Primary (citable) accession number: Q3IS99

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program