Q3IS14 (GUAAB_NATPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B
EC=6.3.5.2

Alternative name(s):
GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	NP1970A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_1000048381
Regions
	Domain	2 – 185	184	GMPS ATP-PPase
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3IS14 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 1A44728E2E63514E	FASTA	305	33,885
	10 20 30 40 50 60 MVEPTAFIDE KIAEIADDVG DANAVIALSG GVDSSTAAAL AYEAIGDQLT PVYVDTGLMR 70 80 90 100 110 120 KGETDQIRDT FDYMDSLRII DARDRFLDAL SGITDPEEKR HAIGEQFIRE FETVARDVDA 130 140 150 160 170 180 DYLVQGTIYP DRIESEGTIK SHHNVGGLPE VVDFEGIVEP MRDLYKDEVR EVARELDLES 190 200 210 220 230 240 IIAERMPFPG PGLAIRVLGE VTDEKLAVAR EANHVVEEEL EEYEPWQALA AVLGKATGVK 250 260 270 280 290 300 GDNRVHGWVV SVRSVESRDG MTARAQELDW ETLQRIQSRI TGENENVARV VYDVTHKPPA TIEYE

References

[1]

"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed: 16169924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2160 / ATCC 35678.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936257 Genomic DNA. Translation: CAI49076.1.
RefSeq	YP_326639.1. NC_007426.1.
3D structure databases
ProteinModelPortal	Q3IS14.
SMR	Q3IS14. Positions 3-305.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IS14.
Proteomic databases
PRIDE	Q3IS14.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3702783.
GenomeReviews	Gene locus NP1970A in contig CR936257_GR.
KEGG	nph:NP1970A.
NMPDR	fig|348780.3.peg.1178.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04539.
HOGENOM	HBG391775.
OMA	QGTIAPD.
ProtClustDB	PRK00919.
Enzyme and pathway databases
BioCyc	NPHA348780:NP1970A-MONOMER.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit.
PROSITE	PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAB_NATPD

Accession

Primary (citable) accession number: Q3IS14

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 8, 2005
Last modified:	December 14, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program