Q3IQZ9 (KATG_NATPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	NP2708A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 713	713	Catalase-peroxidase HAMAP MF_01961		PRO_0000354970
Sites
	Active site	78	1	Proton acceptor By similarity
	Metal binding	241	1	Iron (heme axial ligand) By similarity
	Site	74	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	77 ↔ 200		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-226) By similarity
	Cross-link	200 ↔ 226		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-77) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3IQZ9 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 90FA3F659414A865	FASTA	713	79,455
	10 20 30 40 50 60 MSIDYKKLWP EALDFESLDQ NAQHVDPKDE DFDYAEAFQE LDLEEVKQDI ESVMTDSQEW 70 80 90 100 110 120 WPADYGHYGP LFIRMAWHSA GTYRTTDGRG GASGGYQRLP PVDSWPDNAN LDKARRVLWP 130 140 150 160 170 180 VKQKYGQNLS WADLIVLAGN VALESMGFET FGFAGGREDD FAPDESVDWG PEEEMEASDR 190 200 210 220 230 240 YDEAGELPEP LGATVMGLIY VNPEGPDGEP DLEGSAANIR ESFGRMAMND EETVALIAGG 250 260 270 280 290 300 HTFGKVHGAD DPDEHVGGPP ADAPIDLQGL GWENDFGEGK GPDTITSGIE GPWNTTPTQW 310 320 330 340 350 360 DMSYIDNLLD YEWWPEKGPG GAWQWTTESG ELDAAAPSVD GSSEKEDVMM LTTDVALKRD 370 380 390 400 410 420 PDYREVLERF QENPDEFQEA FAKAWYKLIH RDMGPPERFL GPEVPEETLI WQDPLPDADY 430 440 450 460 470 480 DSIGDEEVAE LKEALLDSEL SVAQLVKTAW ASASTYRDSD KRGGANGARI RLEPQRSWEV 490 500 510 520 530 540 NEPAALADAL ETYEAIQEEF NSARSDAVRV SLADLIVLGG NAAVEQAAAD AGYDVTVPFE 550 560 570 580 590 600 PGRTDATPEQ TDVESFEALK PKADGFRNYL SDEAERKPEE LLVDKADLLN LTPPEMTVLV 610 620 630 640 650 660 GGMRALGATY QDTDRGVFTD EPGTLTNDFF VNILDMDYEW EPVSEDREVF ELRDRETGEV 670 680 690 700 710 EWEGTRFDLI FGSDSRLRAI SEVYGADDGE AEFVEDFVDT WSKVMKLDRF DLE

References

[1]

"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed: 16169924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2160 / ATCC 35678.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936257 Genomic DNA. Translation: CAI49445.1.
RefSeq	YP_327004.1. NC_007426.1.
3D structure databases
HSSP	HSSP built from PDB template 2CCA based on UniProtKB Q50555.
ProteinModelPortal	Q3IQZ9.
SMR	Q3IQZ9. Positions 9-713.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IQZ9.
Protein family/group databases
PeroxiBase	2515. NpCP01.
Proteomic databases
PRIDE	Q3IQZ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3703016.
GenomeReviews	Gene locus NP2708A in contig CR936257_GR.
KEGG	nph:NP2708A.
NMPDR	fig|348780.3.peg.1341.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05214.
HOGENOM	HBG285610.
OMA	KRHAPSM.
PhylomeDB	Q3IQZ9.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	NPHA348780:NP2708A-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_NATPD

Accession

Primary (citable) accession number: Q3IQZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 8, 2005
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program