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Protein

Enolase

Gene

eno

Organism
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pykA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei146SubstrateUniRule annotation1
Binding sitei155SubstrateUniRule annotation1
Active sitei196Proton donorUniRule annotation1
Metal bindingi232MagnesiumUniRule annotation1
Metal bindingi273MagnesiumUniRule annotation1
Binding sitei273SubstrateUniRule annotation1
Metal bindingi300MagnesiumUniRule annotation1
Binding sitei300SubstrateUniRule annotation1
Active sitei325Proton acceptorUniRule annotation1
Binding sitei325Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei376SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciNPHA348780:G1GI2-1413-MONOMER
UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:NP_2846A
OrganismiNatronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis)
Taxonomic identifieri348780 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHaloarculaceaeNatronomonas
Proteomesi
  • UP000002698 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002671471 – 398EnolaseAdd BLAST398

Proteomic databases

PRIDEiQ3IQT0

Interactioni

Protein-protein interaction databases

STRINGi348780.NP2846A

Structurei

3D structure databases

ProteinModelPortaliQ3IQT0
SMRiQ3IQT0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni352 – 355Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01169 Archaea
COG0148 LUCA
HOGENOMiHOG000072173
KOiK01689
OMAiQEFLVVP
OrthoDBiPOG093Z03D9

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q3IQT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLITEVRLR KILDSRGNPT VEAEVRTENG GFGRGAAPSG ASTGEYEAVE
60 70 80 90 100
RPAEEAIAQA RELAVPRIEG QVYAGDQRGV DNALHGADGT DDFSEIGANS
110 120 130 140 150
AVAISMAAAK AAADVLGAPL YQHLGGAFRG RDFPVPLGNV IGGGEHAADA
160 170 180 190 200
THIQEFLAAP VGAPSVADAV FANAKVHGEV QRQLDERGVP AAKGDEGAWA
210 220 230 240 250
PSIDDSEAFE IMEEAIAAVE DEVGFEIRFG LDVAGAELYE DGVYHYGDET
260 270 280 290 300
RSPDEQVAYI ADLVEEYNLV YVEDPLDEDD FSGFAELTER VGDRTLICGD
310 320 330 340 350
DLFVTNTERL QRGIDDGAAN SILIKPNQIG TLSDAFDAIE LATRNGYDPV
360 370 380 390
VSHRSGETED TTIAHLAVAT DAPFIKTGAV GGERTAKLNE LIRIEANA
Length:398
Mass (Da):42,112
Last modified:November 8, 2005 - v1
Checksum:i9274F41A64FB9B25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR936257 Genomic DNA Translation: CAI49514.1
RefSeqiWP_011323139.1, NC_007426.1

Genome annotation databases

GeneIDi3702674
KEGGinph:NP_2846A

Similar proteinsi

Entry informationi

Entry nameiENO_NATPD
AccessioniPrimary (citable) accession number: Q3IQT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health