Q3IQL3 (PURL_NATPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	NP2982A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 713	713	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000236677
Regions
	Nucleotide binding	98 – 109	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3IQL3 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: BFF44EB3212E842A	FASTA	713	74,250
	10 20 30 40 50 60 MSLSPSDREL VTEELGREPT PAEEALFENL WSEHCAYRSS QPLLSAFESE GDRVVVGPGD 70 80 90 100 110 120 DAAVVALPDP ETGENSDTYI TMGIESHNHP SYVDPFDGAA TGVGGIVRDT MSMGAYPIAL 130 140 150 160 170 180 ADSLYFGDFD REHSKYLFEG VVEGISHYGN CIGVPTVTGS VAFHDDYEGN PLVNVACVGL 190 200 210 220 230 240 TDDERLVTAE AQTPGNKLVL FGNATGRDGL GGASFASEDL DEDAETEDRP AVQVGDPYAE 250 260 270 280 290 300 KRLIEANEEL VDDSLVRAAR DLGAAGLGGA SSELVAKGGL GAHIELDRVH QREPNMNALE 310 320 330 340 350 360 ILLAESQERM CYEVRPEDVD AVAAVADKYD LGCSVIGDVT DGNYVCTFDG ETVVDCDAEY 370 380 390 400 410 420 LADGAPMNDL DHVEPTQPDR DRPSPDLETA FEAVVAAPNT ASKEWVYRQY DHEVGTRTAL 430 440 450 460 470 480 KPGDDAALMA VREAGVGLAF SSGADPNWTD TAPYDGARAV ALENATNIAA KGALPLAAVD 490 500 510 520 530 540 CLNGGNPEKP DVYGGFRGIV NGLADMCSTL DVPVVGGNVS LYNDSPSGPI PPTPTLAMTG 550 560 570 580 590 600 TKPGYDAPPA ALSGDGDLLV VGDGGDLELG GSELLAQFGG SDQFPALPDS PAAFIEAVAD 610 620 630 640 650 660 IADLDSTHAT HDVSHGGLAV ALAELVGDAG ADVDLAGSPD ALSVLFSEAV GRVVVETTDP 670 680 690 700 710 EAVKERLDGV APVEHIGHAT DSGRLELSVG DETLSYSAAD IASLRSVIGE TLE

References

[1]

"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed: 16169924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2160 / ATCC 35678.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936257 Genomic DNA. Translation: CAI49582.1.
RefSeq	YP_327140.1. NC_007426.1.
3D structure databases
ProteinModelPortal	Q3IQL3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IQL3.
Proteomic databases
PRIDE	Q3IQL3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3703073.
GenomeReviews	Gene locus NP2982A in contig CR936257_GR.
KEGG	nph:NP2982A.
NMPDR	fig|348780.3.peg.1477.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04101.
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q3IQL3.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	NPHA348780:NP2982A-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_NATPD

Accession

Primary (citable) accession number: Q3IQL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program