Reviewed, UniProtKB/Swiss-Prot Q3IPX0 (DNLI_NATPD)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	NP3474A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein seals, during DNA replication, DNA recombination and DNA repair, nicks in double-stranded DNA By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	DNA ligase		PRO_1000049874
Sites
	Active site	254	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3IPX0-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 621F22FEEA73D814	FASTA	548	58,865
	10 20 30 40 50 60 MEFAEFAARA AEIESEPADL ETVDLVATLC ADAGDDLGTV ARFVQGRVFP AHDSTKLDIG 70 80 90 100 110 120 PSLCYEALAK AAGPNVDSDD IERRLAEVGE IGAVAESLDL GGQQGLAAFG GGTDREALTV 130 140 150 160 170 180 SGVEETLQAL AAAAGDGSTD AKRDRLFDLF NRAEPTEARF LARLVLGEMR VGVGEGTVRD 190 200 210 220 230 240 AIAAAFEVPE PDVERALQVA NDCGLVAETA RDDGAAGLDD IGLVVGRPVK AMLAQAGTAV 250 260 270 280 290 300 EALDEWGTAA VETKYDGARV QVHYDGDSAR LFSRNLEEVT EPLPEVVEFV EQSLSVPAIL 310 320 330 340 350 360 DGEVVAVDDD GTPRPFQEIL RRFRRKHDVA AAREDVSVEL YAFDCLHVDG EDLLDTPLSE 370 380 390 400 410 420 RHDRLESLLA DGVSPLSYAD DADEVADIEA DALDAGHEGI MLKNPDSTYT PGSRGKNWLK 430 440 450 460 470 480 RKPDVETLDL VVTGAEWGEG RRANLLGTFV VSARADDGYE PVGKVATGIT DEELERLHER 490 500 510 520 530 540 LQPHVRTEDG TDVDIEPAVV FEVGYEEIQQ SPTYGSGYAL RFPRFVGVRE DKSPSDADSL ERIDRLTS

References

[1]	"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed: 16169924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR936257 Genomic DNA. Translation: CAI49828.1.
RefSeq	YP_327383.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3702886.
GenomeReviews	Gene locus NP3474A in contig CR936257_GR.
KEGG	nph:NP3474A.
NMPDR	fig|348780.3.peg.1913.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3IPX0.
Enzyme and pathway databases
BioCyc	NPHA348780:NP3474A-MON.
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_NATPD

Accession

Primary (citable) accession number: Q3IPX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 8, 2005
Last modified:	November 25, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)