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Q3IPL2 (SYE_NATPD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:NP_3694A
OrganismNatronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis) [Complete proteome] [HAMAP]
Taxonomic identifier348780 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeNatronomonas

Protein attributes

Sequence length571 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 571571Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000237425

Regions

Motif114 – 12411"HIGH" region HAMAP-Rule MF_00022

Sequences

Sequence LengthMass (Da)Tools
Q3IPL2 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 80D88F8AB47E9D12

FASTA57164,418
        10         20         30         40         50         60 
MDEDLRERIE REAETAALFN ALKHESDAQV GAILGPMMGE NPDFRPHGDE IPGIISPVIE 

        70         80         90        100        110        120 
RVNGLSDEEK RERLEELAPE KVEALAAEDE GDDRPLPELP NADAYDDVRM RLAPNPNGPW 

       130        140        150        160        170        180 
HVGHARMPAV IGTYKDRYDG WFCVRFDDTD PETKRPDLDA YGEILDAIDY LGFEPDEVVK 

       190        200        210        220        230        240 
ASDRVETYYE YGRKLIKAGG AYTCSCDAES FSELKNNAEA CPHRDKDIET TLSEFEAMID 

       250        260        270        280        290        300 
GEYSAGEMVL RVRTDIEHKN PALRDWVAFR MVDTPHPREE AADYRCWPML DFQSGIDDHE 

       310        320        330        340        350        360 
FGISHIIRGI DLQDSAKRQQ FVYEYFDWEY PEVIHWGHVQ IDAYDIEMST STIKQLIDDG 

       370        380        390        400        410        420 
ELDDWDDPRA PTVASLKRRG IRGQAIVNAM VELGTSTSNV DLAMSSVYAE NREFVDDEAD 

       430        440        450        460        470        480 
RRFFVRDGVE KPLAGGPESA SPPLHPNDED RGRREIPVGD AVYVEPDDVP ADGERVWLKG 

       490        500        510        520        530        540 
LGPVRHEDGA FVATDDDIEV VRDGAVDVVH WVPAQESVPL RLRTVDGDEA GHAEPGIETY 

       550        560        570 
DADDIVQFER IGFARIDRHD DEESVAYFAH P 

« Hide

References

[1]"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis."
Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D.
Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35678 / DSM 2160.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR936257 Genomic DNA. Translation: CAI49938.1.
RefSeqYP_327491.1. NC_007426.1.

3D structure databases

ProteinModelPortalQ3IPL2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING348780.NP3694A.

Proteomic databases

PRIDEQ3IPL2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI49938; CAI49938; NP_3694A.
GeneID3702761.
KEGGnph:NP3694A.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000073585.
KOK01885.
OMAYLGWDDP.

Enzyme and pathway databases

BioCycNPHA348780:GJX0-1874-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_A. Glu_tRNA_synth_A.
InterProIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00463. gltX_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_NATPD
AccessionPrimary (citable) accession number: Q3IPL2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 8, 2005
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries