Q3IPL2 (SYE_NATPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	NP_3694A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas › Natronomonas pharaonis

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_0000237425
Regions
	Motif	114 – 124	11	"HIGH" region HAMAP-Rule MF_00022

Sequences

	Sequence		Length	Mass (Da)
	Q3IPL2 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 80D88F8AB47E9D12	FASTA	571	64,418
	10 20 30 40 50 60 MDEDLRERIE REAETAALFN ALKHESDAQV GAILGPMMGE NPDFRPHGDE IPGIISPVIE 70 80 90 100 110 120 RVNGLSDEEK RERLEELAPE KVEALAAEDE GDDRPLPELP NADAYDDVRM RLAPNPNGPW 130 140 150 160 170 180 HVGHARMPAV IGTYKDRYDG WFCVRFDDTD PETKRPDLDA YGEILDAIDY LGFEPDEVVK 190 200 210 220 230 240 ASDRVETYYE YGRKLIKAGG AYTCSCDAES FSELKNNAEA CPHRDKDIET TLSEFEAMID 250 260 270 280 290 300 GEYSAGEMVL RVRTDIEHKN PALRDWVAFR MVDTPHPREE AADYRCWPML DFQSGIDDHE 310 320 330 340 350 360 FGISHIIRGI DLQDSAKRQQ FVYEYFDWEY PEVIHWGHVQ IDAYDIEMST STIKQLIDDG 370 380 390 400 410 420 ELDDWDDPRA PTVASLKRRG IRGQAIVNAM VELGTSTSNV DLAMSSVYAE NREFVDDEAD 430 440 450 460 470 480 RRFFVRDGVE KPLAGGPESA SPPLHPNDED RGRREIPVGD AVYVEPDDVP ADGERVWLKG 490 500 510 520 530 540 LGPVRHEDGA FVATDDDIEV VRDGAVDVVH WVPAQESVPL RLRTVDGDEA GHAEPGIETY 550 560 570 DADDIVQFER IGFARIDRHD DEESVAYFAH P

References

[1]

"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2160 / ATCC 35678.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936257 Genomic DNA. Translation: CAI49938.1.
RefSeq	YP_327491.1. NC_007426.1.
3D structure databases
ProteinModelPortal	Q3IPL2.
ModBase	Search...
Protein-protein interaction databases
STRING	348780.NP3694A.
Proteomic databases
PRIDE	Q3IPL2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAI49938; CAI49938; NP_3694A.
GeneID	3702761.
KEGG	nph:NP3694A.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000073585.
KO	K01885.
OMA	MRFAPNP.
ProtClustDB	PRK04156.
Enzyme and pathway databases
BioCyc	NPHA348780:GJX0-1874-MONOMER.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_A. Glu_tRNA_synth_A.
InterPro	IPR004526. Glu-tRNA-synth_Ib_arc/euk. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR022888. Glu_tRNA_synth_arc. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00463. gltX_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_NATPD

Accession

Primary (citable) accession number: Q3IPL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program