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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNPHA348780:GJX0-1874-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:NP_3694A
OrganismiNatronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
Taxonomic identifieri348780 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeNatronomonas
ProteomesiUP000002698 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 571571Glutamate--tRNA ligasePRO_0000237425Add
BLAST

Proteomic databases

PRIDEiQ3IPL2.

Interactioni

Protein-protein interaction databases

STRINGi348780.NP3694A.

Structurei

3D structure databases

ProteinModelPortaliQ3IPL2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi114 – 12411"HIGH" regionAdd
BLAST

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000073585.
KOiK01885.
OMAiGEYSGWD.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A.
InterProiIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3IPL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEDLRERIE REAETAALFN ALKHESDAQV GAILGPMMGE NPDFRPHGDE
60 70 80 90 100
IPGIISPVIE RVNGLSDEEK RERLEELAPE KVEALAAEDE GDDRPLPELP
110 120 130 140 150
NADAYDDVRM RLAPNPNGPW HVGHARMPAV IGTYKDRYDG WFCVRFDDTD
160 170 180 190 200
PETKRPDLDA YGEILDAIDY LGFEPDEVVK ASDRVETYYE YGRKLIKAGG
210 220 230 240 250
AYTCSCDAES FSELKNNAEA CPHRDKDIET TLSEFEAMID GEYSAGEMVL
260 270 280 290 300
RVRTDIEHKN PALRDWVAFR MVDTPHPREE AADYRCWPML DFQSGIDDHE
310 320 330 340 350
FGISHIIRGI DLQDSAKRQQ FVYEYFDWEY PEVIHWGHVQ IDAYDIEMST
360 370 380 390 400
STIKQLIDDG ELDDWDDPRA PTVASLKRRG IRGQAIVNAM VELGTSTSNV
410 420 430 440 450
DLAMSSVYAE NREFVDDEAD RRFFVRDGVE KPLAGGPESA SPPLHPNDED
460 470 480 490 500
RGRREIPVGD AVYVEPDDVP ADGERVWLKG LGPVRHEDGA FVATDDDIEV
510 520 530 540 550
VRDGAVDVVH WVPAQESVPL RLRTVDGDEA GHAEPGIETY DADDIVQFER
560 570
IGFARIDRHD DEESVAYFAH P
Length:571
Mass (Da):64,418
Last modified:November 8, 2005 - v1
Checksum:i80D88F8AB47E9D12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR936257 Genomic DNA. Translation: CAI49938.1.
RefSeqiWP_011323556.1. NC_007426.1.
YP_327491.1. NC_007426.1.

Genome annotation databases

EnsemblBacteriaiCAI49938; CAI49938; NP_3694A.
GeneIDi3702761.
KEGGinph:NP3694A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR936257 Genomic DNA. Translation: CAI49938.1.
RefSeqiWP_011323556.1. NC_007426.1.
YP_327491.1. NC_007426.1.

3D structure databases

ProteinModelPortaliQ3IPL2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi348780.NP3694A.

Proteomic databases

PRIDEiQ3IPL2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI49938; CAI49938; NP_3694A.
GeneIDi3702761.
KEGGinph:NP3694A.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000073585.
KOiK01885.
OMAiGEYSGWD.

Enzyme and pathway databases

BioCyciNPHA348780:GJX0-1874-MONOMER.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A.
InterProiIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis."
    Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D.
    Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35678 / DSM 2160.

Entry informationi

Entry nameiSYE_NATPD
AccessioniPrimary (citable) accession number: Q3IPL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 8, 2005
Last modified: April 29, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.