Q3IP73 (PROB_NATPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	NP_3976A

Organism

Natronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas › Natronomonas pharaonis

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Glutamate 5-kinase HAMAP-Rule MF_00456		PRO_0000230077
Regions
	Nucleotide binding	190 – 191	2	ATP By similarity
Sites
	Binding site	33	1	ATP By similarity
	Binding site	73	1	Substrate By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	170	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3IP73 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 8E755E552D667BB1	FASTA	284	30,584
	10 20 30 40 50 60 MSETIADELG SVPQSVVEAA REDAANAKRV VVKAGTNSLT DEESNLDDDK LDKLVDDIAD 70 80 90 100 110 120 LLERDKDVLL VSSGAVGAGI GRVGYGSRTV EESQALSTVG QSHLMRRYTE SFERYDRKVA 130 140 150 160 170 180 QILLTQQDLE NPERFTNFCN TVETLLEWDI VPIINENDAV ATQEIRIGDN DMLSSSVAVG 190 200 210 220 230 240 VDADLLVTLT DVDAVYTGNP KEDPDAERIE AVGENYDQIQ GLVEESSSSD FGGIRTKVEG 250 260 270 280 ARNVSEYGIP AIIAGSAEPD VLQQIATGKS VGTLFVPING ETDD

References

[1]

"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35678 / DSM 2160.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936257 Genomic DNA. Translation: CAI50079.2.
RefSeq	YP_327630.1. NC_007426.1.
3D structure databases
ProteinModelPortal	Q3IP73.
ModBase	Search...
Protein-protein interaction databases
STRING	348780.NP3976A.
Proteomic databases
PRIDE	Q3IP73.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAI50079; CAI50079; NP_3976A.
GeneID	3703055.
KEGG	nph:NP3976A.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HOG000246368.
KO	K00931.
OMA	VYTGNPK.
ProtClustDB	CLSK760159.
Enzyme and pathway databases
BioCyc	NPHA348780:GJX0-2018-MONOMER.
UniPathway	UPA00098; UER00359.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit.
HAMAP	MF_00456. ProB.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_NATPD

Accession

Primary (citable) accession number: Q3IP73

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 8, 2005
Last modified:	May 29, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program