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Protein

Glutamate 5-kinase

Gene

proB

Organism
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPUniRule annotation1
Binding sitei73SubstrateUniRule annotation1
Binding sitei158SubstrateUniRule annotation1
Binding sitei170Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi190 – 191ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNPHA348780:G1GI2-1979-MONOMER
UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:NP_3976A
OrganismiNatronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis)
Taxonomic identifieri348780 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHaloarculaceaeNatronomonas
Proteomesi
  • UP000002698 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002300771 – 284Glutamate 5-kinaseAdd BLAST284

Proteomic databases

PRIDEiQ3IP73

Interactioni

Protein-protein interaction databases

STRINGi348780.NP3976A

Structurei

3D structure databases

ProteinModelPortaliQ3IP73
SMRiQ3IP73
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00864 Archaea
COG0263 LUCA
HOGENOMiHOG000246368
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG093Z03ES

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit

Sequencei

Sequence statusi: Complete.

Q3IP73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETIADELG SVPQSVVEAA REDAANAKRV VVKAGTNSLT DEESNLDDDK
60 70 80 90 100
LDKLVDDIAD LLERDKDVLL VSSGAVGAGI GRVGYGSRTV EESQALSTVG
110 120 130 140 150
QSHLMRRYTE SFERYDRKVA QILLTQQDLE NPERFTNFCN TVETLLEWDI
160 170 180 190 200
VPIINENDAV ATQEIRIGDN DMLSSSVAVG VDADLLVTLT DVDAVYTGNP
210 220 230 240 250
KEDPDAERIE AVGENYDQIQ GLVEESSSSD FGGIRTKVEG ARNVSEYGIP
260 270 280
AIIAGSAEPD VLQQIATGKS VGTLFVPING ETDD
Length:284
Mass (Da):30,584
Last modified:November 8, 2005 - v1
Checksum:i8E755E552D667BB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR936257 Genomic DNA Translation: CAI50079.2
RefSeqiWP_011323695.1, NC_007426.1

Genome annotation databases

GeneIDi3703055
KEGGinph:NP_3976A

Similar proteinsi

Entry informationi

Entry nameiPROB_NATPD
AccessioniPrimary (citable) accession number: Q3IP73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 8, 2005
Last modified: May 23, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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