Q3IP72 (PROA_NATPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	NP3978A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000230035

Sequences

	Sequence		Length	Mass (Da)
	Q3IP72 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: BB01CE7C2483D92F	FASTA	438	47,559
	10 20 30 40 50 60 MTETTEAKVD AAQTAALELA NESDEARQEN LQAIADAIDE RRAEVLEANE KDVEAAEEML 70 80 90 100 110 120 EAGEYSQALV DRLKLSDAKL DSIIEMVRSV AGQEDPLGKT LTARELDDGL DLYKVSVPIG 130 140 150 160 170 180 VIGTVFESRP DALVQIAALS LRSGNAVILK GGSEASHSNR VLYEIIREAT ADLPDGWAQL 190 200 210 220 230 240 IEAREDVDRL LGMDDSVDLL MPRGSSAFVS YIQDNTSIPV LGHTEGVCHV YVDDAADLDM 250 260 270 280 290 300 ATDIAYDAKV QYPAVCNAVE TLLVHEDVAE EYLPDIAARY AEADVEMRGD EATRSVLDRD 310 320 330 340 350 360 IEAATDDDWT SEYGDLIVAI KVVDSLESAI DHINTNGSKH TESIVTEDDG RASTFMRRLD 370 380 390 400 410 420 SASVFHNAST RFSDGYRFGL GAEVGISTGK IHARGPVGLK GLTTYKYHLE GDGHLVATYA 430 GEDAKPFSHE EFDGEWSP

References

[1]

"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed: 16169924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2160 / ATCC 35678.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR936257 Genomic DNA. Translation: CAI50080.1.
RefSeq	YP_327631.1. NC_007426.1.
3D structure databases
ProteinModelPortal	Q3IP72.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IP72.
Proteomic databases
PRIDE	Q3IP72.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3703054.
GenomeReviews	Gene locus NP3978A in contig CR936257_GR.
KEGG	nph:NP3978A.
NMPDR	fig|348780.3.peg.2561.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05521.
HOGENOM	HBG318080.
OMA	QYPAACN.
PhylomeDB	Q3IP72.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	NPHA348780:NP3978A-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_NATPD

Accession

Primary (citable) accession number: Q3IP72

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 8, 2005
Last modified:	November 16, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program