Reviewed, UniProtKB/Swiss-Prot Q3ING6 (G1PDH_NATPD)

Names and origin

Protein names

Recommended name:
    Glycerol-1-phosphate dehydrogenase [NAD(P)+]
      Short name=G1P dehydrogenase
      Short name=G1PDH
    EC=1.1.1.261
Alternative name(s):
    sn-glycerol-1-phosphate dehydrogenase
    Enantiomeric glycerophosphate synthase

Gene names

Name:	egsA
Ordered Locus Names:	NP4492A

Organism

Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) [Complete proteome] [HAMAP]

Taxonomic identifier

348780 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity.
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-dehydroquinate synthase activity Inferred from electronic annotation. Source: InterPro glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497		PRO_1000050605
Regions
	Nucleotide binding	98 – 102	5	NAD By similarity
	Nucleotide binding	120 – 123	4	NAD By similarity
Sites
	Metal binding	172	1	Zinc; catalytic By similarity
	Metal binding	252	1	Zinc; catalytic By similarity
	Metal binding	268	1	Zinc; catalytic By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	129	1	NAD By similarity
	Binding site	172	1	Substrate By similarity
	Binding site	256	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ING6-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: D6962B4807DAB534	FASTA	352	37,445
	10 20 30 40 50 60 MFEKSTWIRL PRSVVVGHGV LDRTAEAVEE LYLDGRPLIV TSPTPEELAV DRLREQFVSA 70 80 90 100 110 120 GFDPAVAVVE EASFDAVERV IETAERETAG FLVGFGGGKP IDIAKMAADE RNCGFISVPT 130 140 150 160 170 180 AASHDGIVSG RGSVPEGDTR HSVAAHPPLA VIADTELIAN SPWRLTTAGC ADIISNYTAV 190 200 210 220 230 240 KDWQLAHRLK NVEYSEYAGA LSQMTAEMLV DNSASIKRGL EESAWIVVKA LVSSGVAMSI 250 260 270 280 290 300 AGSSRPASGA EHLISHQLDR IAPETALHGH QVGVASIVTE YLHSGEGGDW RRVRDALASI 310 320 330 340 350 DAPTTAAELG IDGERFIEAT TSAHEIRDRY TILGDGIERE AAIEAAATTG VL

References

[1]	"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis." Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. Genome Res. 15:1336-1343(2005) [PubMed: 16169924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR936257 Genomic DNA. Translation: CAI50337.1.
RefSeq	YP_330974.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ING6.
Genome annotation databases
GeneID	3702751.
GenomeReviews	Gene locus NP4492A in contig CR936257_GR.
KEGG	nph:NP4492A.
NMPDR	fig|348780.3.peg.2396.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3ING6.
OMA	ADWELAH.
Enzyme and pathway databases
BioCyc	NPHA348780:NP4492A-MON.
Family and domain databases
HAMAP	MF_00497. [Tree]
InterPro	IPR002658. DHQ_synth_AroB. IPR016205. Glycerol_DH. [Graphical view]
Pfam	PF01761. DHQ_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000112. Glycerol_dehydrogenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G1PDH_NATPD

Accession

Primary (citable) accession number: Q3ING6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)