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Q3IMN2 (KAE1B_NATPD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including the following 2 domains:

  1. tRNA N6-adenosine threonylcarbamoyltransferase
    EC=2.6.99.4
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthase
    Short name=t(6)A synthase
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
  2. Serine/threonine-protein kinase Bud32
    EC=2.7.11.1
Gene names
Ordered Locus Names:NP_5070A
OrganismNatronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis) [Complete proteome] [HAMAP]
Taxonomic identifier348780 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeNatronomonas

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity. HAMAP-Rule MF_01447

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_01447

L-threonylcarbamoyladenylate + adenine37 in tRNA = AMP + N(6)-L-threonylcarbamoyladenine37 in tRNA. HAMAP-Rule MF_01447

Cofactor

Binds 1 Fe2+ ion per subunit By similarity. HAMAP-Rule MF_01447

Subunit structure

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01447.

Sequence similarities

In the N-terminal section; belongs to the KAE1 / TsaD family.

In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein HAMAP-Rule MF_01447
PRO_0000303659

Regions

Domain338 – 533196Protein kinase
Nucleotide binding345 – 3528ATP By similarity
Region1 – 329329Kae1 HAMAP-Rule MF_01447
Region134 – 1385Threonylcarbamoyl-AMP binding By similarity

Sites

Active site4521Proton acceptor; for kinase activity By similarity
Metal binding1131Iron By similarity
Metal binding1171Iron By similarity
Metal binding2901Iron By similarity
Binding site1661Threonylcarbamoyl-AMP By similarity
Binding site1791Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding site1831Threonylcarbamoyl-AMP By similarity
Binding site2621Threonylcarbamoyl-AMP By similarity
Binding site3631ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3IMN2 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 03E030815ECABE24

FASTA53357,263
        10         20         30         40         50         60 
MTRVLGIEGT AWCASAAVFD AETDAVFIDS DAYVPESGGI HPREAAEHMR EAVPSVVEAA 

        70         80         90        100        110        120 
LDHVESNWGD PADAIDAVAF SRGPGLGPCL RIAGTAARSL AGTLSCPLVG VNHMVAHLEI 

       130        140        150        160        170        180 
GRHRSGFESP VCLNASGANA HVLGYHNGRY RVLGETMDTG VGNAIDKFTR HVGWSHPGGP 

       190        200        210        220        230        240 
KVESHAEDGD YVELPYVVKG MDFSFSGIMS AAKQAYDDGT PVADVCCGLQ ETIFAMLAEV 

       250        260        270        280        290        300 
SERALSLTGA DELVVGGGVA QNSRLQEMLT QMCENRGAAI YVPEPRFLRD NAGMIAVLGA 

       310        320        330        340        350        360 
KMYEAGDIIS IPESGVRPDF RPDEVPVSWR DDEAVARPVP TDERRQGAEA VVDIDADGGR 

       370        380        390        400        410        420 
VTKRRLEKAY RHPVLDSRLR SQRTRSEARL TSEARRQGVP TPVVYDVDPD AGRLVFQYVG 

       430        440        450        460        470        480 
DADLKTALSE SAVRDVGRHL AACHAAGFVH GDPTPRNVRV GEDRAFLIDF GLGYYTDAVE 

       490        500        510        520        530 
DYAMDLHVFE GALGGTADDP TAQITAFEDA YRSAGDGAVV DHLREIETRG RYQ 

« Hide

References

[1]"Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis."
Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D.
Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35678 / DSM 2160.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR936257 Genomic DNA. Translation: CAI50626.1.
RefSeqYP_331258.1. NC_007426.1.

3D structure databases

ProteinModelPortalQ3IMN2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING348780.NP5070A.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI50626; CAI50626; NP_5070A.
GeneID3702246.
KEGGnph:NP5070A.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0533.
HOGENOMHOG000109569.
KOK15904.
OMARDNAGMI.

Enzyme and pathway databases

BioCycNPHA348780:GJX0-2570-MONOMER.

Family and domain databases

HAMAPMF_01447. Kae1_Bud32_arch.
InterProIPR002575. Aminoglycoside_PTrfase.
IPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
[Graphical view]
PfamPF01636. APH. 1 hit.
PF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSPR00789. OSIALOPTASE.
SUPFAMSSF56112. SSF56112. 1 hit.
TIGRFAMsTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
ProtoNetSearch...

Entry information

Entry nameKAE1B_NATPD
AccessionPrimary (citable) accession number: Q3IMN2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: November 8, 2005
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families