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Q3IMN2

- KAE1B_NATPD

UniProt

Q3IMN2 - KAE1B_NATPD

Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

NP_5070A

Organism
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.UniRule annotation
    L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi113 – 1131IronUniRule annotation
    Metal bindingi117 – 1171IronUniRule annotation
    Binding sitei166 – 1661Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei179 – 1791Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation
    Binding sitei183 – 1831Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei262 – 2621Threonylcarbamoyl-AMPUniRule annotation
    Metal bindingi290 – 2901IronUniRule annotation
    Binding sitei363 – 3631ATPUniRule annotation
    Active sitei452 – 4521Proton acceptor; for kinase activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi345 – 3528ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. iron ion binding Source: UniProtKB-HAMAP
    3. metalloendopeptidase activity Source: InterPro
    4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
    5. protein serine/threonine kinase activity Source: UniProtKB-KW
    6. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
    7. zinc ion binding Source: InterPro

    GO - Biological processi

    1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Iron, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciNPHA348780:GJX0-2570-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
    Including the following 2 domains:
    tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthase
    Short name:
    t(6)A synthase
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
    Gene namesi
    Ordered Locus Names:NP_5070A
    OrganismiNatronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
    Taxonomic identifieri348780 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeNatronomonas
    ProteomesiUP000002698: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 533533Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinPRO_0000303659Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

    Protein-protein interaction databases

    STRINGi348780.NP5070A.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3IMN2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini338 – 533196Protein kinaseUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 329329Kae1Add
    BLAST
    Regioni134 – 1385Threonylcarbamoyl-AMP bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
    In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
    Contains 1 protein kinase domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0533.
    HOGENOMiHOG000109569.
    KOiK15904.
    OMAiRDNAGMI.

    Family and domain databases

    HAMAPiMF_01447. Kae1_Bud32_arch.
    InterProiIPR002575. Aminoglycoside_PTrfase.
    IPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR009220. tRNA_threonyl_synthase/kinase.
    [Graphical view]
    PfamiPF01636. APH. 1 hit.
    PF00814. Peptidase_M22. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSiPR00789. OSIALOPTASE.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q3IMN2-1 [UniParc]FASTAAdd to Basket

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    MTRVLGIEGT AWCASAAVFD AETDAVFIDS DAYVPESGGI HPREAAEHMR    50
    EAVPSVVEAA LDHVESNWGD PADAIDAVAF SRGPGLGPCL RIAGTAARSL 100
    AGTLSCPLVG VNHMVAHLEI GRHRSGFESP VCLNASGANA HVLGYHNGRY 150
    RVLGETMDTG VGNAIDKFTR HVGWSHPGGP KVESHAEDGD YVELPYVVKG 200
    MDFSFSGIMS AAKQAYDDGT PVADVCCGLQ ETIFAMLAEV SERALSLTGA 250
    DELVVGGGVA QNSRLQEMLT QMCENRGAAI YVPEPRFLRD NAGMIAVLGA 300
    KMYEAGDIIS IPESGVRPDF RPDEVPVSWR DDEAVARPVP TDERRQGAEA 350
    VVDIDADGGR VTKRRLEKAY RHPVLDSRLR SQRTRSEARL TSEARRQGVP 400
    TPVVYDVDPD AGRLVFQYVG DADLKTALSE SAVRDVGRHL AACHAAGFVH 450
    GDPTPRNVRV GEDRAFLIDF GLGYYTDAVE DYAMDLHVFE GALGGTADDP 500
    TAQITAFEDA YRSAGDGAVV DHLREIETRG RYQ 533
    Length:533
    Mass (Da):57,263
    Last modified:November 8, 2005 - v1
    Checksum:i03E030815ECABE24
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR936257 Genomic DNA. Translation: CAI50626.1.
    RefSeqiYP_331258.1. NC_007426.1.

    Genome annotation databases

    EnsemblBacteriaiCAI50626; CAI50626; NP_5070A.
    GeneIDi3702246.
    KEGGinph:NP5070A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR936257 Genomic DNA. Translation: CAI50626.1 .
    RefSeqi YP_331258.1. NC_007426.1.

    3D structure databases

    ProteinModelPortali Q3IMN2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 348780.NP5070A.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI50626 ; CAI50626 ; NP_5070A .
    GeneIDi 3702246.
    KEGGi nph:NP5070A.

    Phylogenomic databases

    eggNOGi COG0533.
    HOGENOMi HOG000109569.
    KOi K15904.
    OMAi RDNAGMI.

    Enzyme and pathway databases

    BioCyci NPHA348780:GJX0-2570-MONOMER.

    Family and domain databases

    HAMAPi MF_01447. Kae1_Bud32_arch.
    InterProi IPR002575. Aminoglycoside_PTrfase.
    IPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR009220. tRNA_threonyl_synthase/kinase.
    [Graphical view ]
    Pfami PF01636. APH. 1 hit.
    PF00814. Peptidase_M22. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSi PR00789. OSIALOPTASE.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis."
      Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D.
      Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35678 / DSM 2160.

    Entry informationi

    Entry nameiKAE1B_NATPD
    AccessioniPrimary (citable) accession number: Q3IMN2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3