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Q3IMN2

- KAE1B_NATPD

UniProt

Q3IMN2 - KAE1B_NATPD

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Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

NP_5070A

Organism
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi113 – 1131IronUniRule annotation
Metal bindingi117 – 1171IronUniRule annotation
Binding sitei166 – 1661Threonylcarbamoyl-AMPUniRule annotation
Binding sitei179 – 1791Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation
Binding sitei183 – 1831Threonylcarbamoyl-AMPUniRule annotation
Binding sitei262 – 2621Threonylcarbamoyl-AMPUniRule annotation
Metal bindingi290 – 2901IronUniRule annotation
Binding sitei363 – 3631ATPUniRule annotation
Active sitei452 – 4521Proton acceptor; for kinase activityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi345 – 3528ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. metalloendopeptidase activity Source: InterPro
  4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
  5. protein serine/threonine kinase activity Source: UniProtKB-KW
  6. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNPHA348780:GJX0-2570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
Gene namesi
Ordered Locus Names:NP_5070A
OrganismiNatronomonas pharaonis (strain ATCC 35678 / DSM 2160) (Halobacterium pharaonis)
Taxonomic identifieri348780 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeNatronomonas
ProteomesiUP000002698: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinPRO_0000303659Add
BLAST

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi348780.NP5070A.

Structurei

3D structure databases

ProteinModelPortaliQ3IMN2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini338 – 533196Protein kinaseUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 329329Kae1Add
BLAST
Regioni134 – 1385Threonylcarbamoyl-AMP bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
Contains 1 protein kinase domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0533.
HOGENOMiHOG000109569.
KOiK15904.
OMAiRDNAGMI.

Family and domain databases

HAMAPiMF_01447. Kae1_Bud32_arch.
InterProiIPR002575. Aminoglycoside_PTrfase.
IPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
[Graphical view]
PfamiPF01636. APH. 1 hit.
PF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3IMN2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTRVLGIEGT AWCASAAVFD AETDAVFIDS DAYVPESGGI HPREAAEHMR
60 70 80 90 100
EAVPSVVEAA LDHVESNWGD PADAIDAVAF SRGPGLGPCL RIAGTAARSL
110 120 130 140 150
AGTLSCPLVG VNHMVAHLEI GRHRSGFESP VCLNASGANA HVLGYHNGRY
160 170 180 190 200
RVLGETMDTG VGNAIDKFTR HVGWSHPGGP KVESHAEDGD YVELPYVVKG
210 220 230 240 250
MDFSFSGIMS AAKQAYDDGT PVADVCCGLQ ETIFAMLAEV SERALSLTGA
260 270 280 290 300
DELVVGGGVA QNSRLQEMLT QMCENRGAAI YVPEPRFLRD NAGMIAVLGA
310 320 330 340 350
KMYEAGDIIS IPESGVRPDF RPDEVPVSWR DDEAVARPVP TDERRQGAEA
360 370 380 390 400
VVDIDADGGR VTKRRLEKAY RHPVLDSRLR SQRTRSEARL TSEARRQGVP
410 420 430 440 450
TPVVYDVDPD AGRLVFQYVG DADLKTALSE SAVRDVGRHL AACHAAGFVH
460 470 480 490 500
GDPTPRNVRV GEDRAFLIDF GLGYYTDAVE DYAMDLHVFE GALGGTADDP
510 520 530
TAQITAFEDA YRSAGDGAVV DHLREIETRG RYQ
Length:533
Mass (Da):57,263
Last modified:November 8, 2005 - v1
Checksum:i03E030815ECABE24
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR936257 Genomic DNA. Translation: CAI50626.1.
RefSeqiYP_331258.1. NC_007426.1.

Genome annotation databases

EnsemblBacteriaiCAI50626; CAI50626; NP_5070A.
GeneIDi3702246.
KEGGinph:NP5070A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR936257 Genomic DNA. Translation: CAI50626.1 .
RefSeqi YP_331258.1. NC_007426.1.

3D structure databases

ProteinModelPortali Q3IMN2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 348780.NP5070A.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI50626 ; CAI50626 ; NP_5070A .
GeneIDi 3702246.
KEGGi nph:NP5070A.

Phylogenomic databases

eggNOGi COG0533.
HOGENOMi HOG000109569.
KOi K15904.
OMAi RDNAGMI.

Enzyme and pathway databases

BioCyci NPHA348780:GJX0-2570-MONOMER.

Family and domain databases

HAMAPi MF_01447. Kae1_Bud32_arch.
InterProi IPR002575. Aminoglycoside_PTrfase.
IPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
[Graphical view ]
Pfami PF01636. APH. 1 hit.
PF00814. Peptidase_M22. 1 hit.
[Graphical view ]
PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
PRINTSi PR00789. OSIALOPTASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis."
    Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D.
    Genome Res. 15:1336-1343(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35678 / DSM 2160.

Entry informationi

Entry nameiKAE1B_NATPD
AccessioniPrimary (citable) accession number: Q3IMN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: November 8, 2005
Last modified: October 29, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3