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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43NADUniRule annotation1
Binding sitei70NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi168Iron; shared with adjacent protomerUniRule annotation1
Binding sitei168NAD; shared with adjacent protomerUniRule annotation1
Metal bindingi183IronUniRule annotation1
Binding sitei258NAD; via amide nitrogenUniRule annotation1
Binding sitei268GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 63NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:NP_5174A
OrganismiNatronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis)
Taxonomic identifieri348780 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHaloarculaceaeNatronomonas
Proteomesi
  • UP000002698 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002593821 – 309Thiamine thiazole synthaseAdd BLAST309

Proteomic databases

PRIDEiQ3IMI0.

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi348780.NP5174A.

Structurei

3D structure databases

ProteinModelPortaliQ3IMI0.
SMRiQ3IMI0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
HOGENOMiHOG000106048.
KOiK03146.
OMAiGIVMNWT.
OrthoDBiPOG093Z09PU.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3IMI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEFDQFSDV GEAEVTRAIG QEWTEEFMDF SDSDVIIVGG GPSGLMAAKE
60 70 80 90 100
LSERGVQTMV VEKNNYLGGG FWLGGFLMNK VTVRDPAQNV LEDLDVDFKR
110 120 130 140 150
SQDSEGLYVA NGPEACSGLI KAACDAGAKM QNMTEFTDIV IREDHRVGGI
160 170 180 190 200
VMNWTPVHAL PREITCVDPI AVEADLVIDA TGHDAVAVSK LQERGVLNAP
210 220 230 240 250
GIEHADEHNT GMDQTGDDSY GAPGHDSPGH DSMWVGQSED AVVEHTGLAH
260 270 280 290 300
EGLIVTGMAT ATTYGLPRMG PTFGAMLLSG KRAAQAALDE LGVDAPDVEM

TTRAEPADD
Length:309
Mass (Da):32,910
Last modified:November 8, 2005 - v1
Checksum:i1D96EC83C65DEB4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR936257 Genomic DNA. Translation: CAI50678.1.
RefSeqiWP_011324288.1. NC_007426.1.

Genome annotation databases

EnsemblBacteriaiCAI50678; CAI50678; NP_5174A.
GeneIDi3703243.
KEGGinph:NP_5174A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR936257 Genomic DNA. Translation: CAI50678.1.
RefSeqiWP_011324288.1. NC_007426.1.

3D structure databases

ProteinModelPortaliQ3IMI0.
SMRiQ3IMI0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi348780.NP5174A.

Proteomic databases

PRIDEiQ3IMI0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI50678; CAI50678; NP_5174A.
GeneIDi3703243.
KEGGinph:NP_5174A.

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
HOGENOMiHOG000106048.
KOiK03146.
OMAiGIVMNWT.
OrthoDBiPOG093Z09PU.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_NATPD
AccessioniPrimary (citable) accession number: Q3IMI0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 8, 2005
Last modified: April 12, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.