Q3IKY9 (ACEK1_PSEHT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase 1
Short name=IDH kinase/phosphatase 1
Short name=IDHK/P 1
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK1
Ordered Locus Names:	PSHAa1307

Organism

Pseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]

Taxonomic identifier

326442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Isocitrate dehydrogenase kinase/phosphatase 1 HAMAP MF_00747		PRO_0000288293
Regions
	Nucleotide binding	337 – 343	7	ATP By similarity
Sites
	Active site	393	1	By similarity
	Binding site	358	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3IKY9 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: E076B5CAA8D9BFDA	FASTA	592	68,071
	10 20 30 40 50 60 MPDTITNKLQ NSTVQDVIAT KLARAVFAGF EAMFATFLNI TLGAQSRFEQ RQYHEVQSAM 70 80 90 100 110 120 RERLQVYERQ VKSVSEAVKV IAYAELSCPQ TWQLAKNIYG NMVKNHENEP IAHTFFNSTF 130 140 150 160 170 180 GAIWDDKKIR TVHLFVLKAK YRTQPRPYDS LVKRISLQHG FNSAIKTLIT NQVFRVPFSN 190 200 210 220 230 240 LNQDVATLQA TLTQGAKQQC RQVYELINLN DGYIEYAYSH FYRNKACYLI GRCIAKNGDN 250 260 270 280 290 300 MPFAIAILNT PKGLKIDAVM MGADQLSLLF GFARTYFMVD TDQPARYVDY LSVLMPHKQR 310 320 330 340 350 360 FELFNAIGFI KHAKTEFYRY KVDTTKNSPA SFKYVAAPGT PGMVMLVFTI AGSDHVYKVI 370 380 390 400 410 420 KDKFSAPKTA TKAQVKEKYN FVKQADRVGR LVDTHEFRYL AFDLSRFSEQ LLQQMKEHIG 430 440 450 460 470 480 SSLIISGKAL ILKHVYVERK MTPLNLYIND CDSKALAQVM LDYGRAIKDL AGANIFPGDM 490 500 510 520 530 540 LMKNFGVTRW GRVVFYDYDE ICPLTDCNFR EVPQTQNALE ELSSDSYFDI EPNDIFPSQF 550 560 570 580 590 KVFFSANELA FNAFNSHHSD LFNAQFWQTC QQQVQQGYLP DVYPYKQSWR FK

References

[1]

"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125." Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A. Genome Res. 15:1325-1335(2005) [PubMed: 16169927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TAC 125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR954246 Genomic DNA. Translation: CAI86382.1.
RefSeq	YP_339825.1. NC_007481.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IKY9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3708337.
GenomeReviews	Gene locus PSHAa1307 in contig CR954246_GR.
KEGG	pha:PSHAa1307.
NMPDR	fig|326442.4.peg.993.
PATRIC	32296195. VBIPseHal105694_1263.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
PhylomeDB	Q3IKY9.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	PHAL326442:PSHAA1307-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK1_PSEHT

Accession

Primary (citable) accession number: Q3IKY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program